The Heterotrimeric G Protein Genes of Caenorhabditis elegans

Jansen, Gert; Thijssen, Karen L.; Werner, P.; Horst, M. van der; Hazendonk, Esther; Plasterk, Ronald H.A.

doi:10.1007/978-3-662-04264-9_2

Cited by 51 publications

(95 citation statements)

References 33 publications

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“…Thus far, no function has been described for GPA-1 (Jansen et al, 1999). We found that gpa-1 animals have a defect in gustatory plasticity, whereas they showed wild-type avoidance of 1 M NaCl (results not shown).…”

Section: Resultsmentioning

confidence: 56%

Antagonistic sensory cues generate gustatory plasticity in Caenorhabditis elegans

Hukema

Rademakers

Dekkers

et al. 2006

EMBO J

154

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Caenorhabditis elegans shows chemoattraction to 0.1-200 mM NaCl, avoidance of higher NaCl concentrations, and avoidance of otherwise attractive NaCl concentrations after prolonged exposure to NaCl (gustatory plasticity). Previous studies have shown that the ASE and ASH sensory neurons primarily mediate attraction and avoidance of NaCl, respectively. Here we show that balances between at least four sensory cell types, ASE, ASI, ASH, ADF and perhaps ADL, modulate the response to NaCl. Our results suggest that two NaCl-attraction signalling pathways exist, one of which uses Ca 2 þ /cGMP signalling. In addition, we provide evidence that attraction to NaCl is antagonised by G-protein signalling in the ASH neurons, which is desensitised by the G-protein-coupled receptor kinase GRK-2. Finally, the response to NaCl is modulated by G-protein signalling in the ASI and ADF neurons, a second G-protein pathway in ASH and cGMP signalling in neurons exposed to the body fluid.

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Section: Resultsmentioning

confidence: 56%

Antagonistic sensory cues generate gustatory plasticity in Caenorhabditis elegans

Hukema

Rademakers

Dekkers

et al. 2006

EMBO J

154

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“…Like that of its C. elegans ortholog, gpa-3 (Jansen, et al, 1999), the Ss gpa-3 promoter drives gfp expression predominantly in a cluster of amphidial neurons and in two pairs of phasmidial neurons in L1 (Fig. 1A-D).…”

Section: Discussionmentioning

confidence: 99%

“…1A-D). C. elegans gpa-3 is expressed in eight pairs of chemosensory neurons in the amphidial complex (ADF, ADL, ASE, ASG, ASH, ASI, ASJ and ASK), two pairs of sensory neurons in the phasmids (PHA and PHB) and two other non-sensory cells (Jansen, et al, 1999). The amphidial neurons of S. stercoralis have been described and their homologies with amphidial neurons of C. elegans have been proposed based on morphology, position of cell bodies within the lateral ganglion and, in several cases, function inferred from microlaser ablation studies (Ashton, et al, 1995;Ashton, et al, 1998;Lopez, et al, 2000;Nolan, et al, 2004;Ashton, et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Strongyloides stercoralis: Cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3′ UTR

Junio

Massey

et al. 2008

Experimental Parasitology

116

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Transgenesis is a valuable methodology for studying gene expression patterns and gene function. It has recently become available for research on some parasitic nematodes, including Strongyloides stercoralis. Previously, we described a vector construct, comprising the promoter and 3′ UTR of the S. stercoralis gene Ss era-1 that gives expression of GFP in intestinal cells of developing F1 progeny. In the present study, we identified three new S. stercoralis promoters, which, in combination with the Ss era-1 3′ UTR, can drive expression of GFP or the red fluorescent protein, mRFPmars, in tissuespecific fashion. These include Ss act-2, which drives expression in body wall muscle cells, Ss gpa-3, which drives expression in amphidial and phasmidial neurons and Ss rps-21, which drives ubiquitous expression in F1 transformants and in the gonads of microinjected P0 female worms. Concomitant microinjection of vectors containing GFP and mRFPmars gave dually transformed F1 progeny, suggesting that these constructs could be used as co-injection markers for other transgenes of interest. We have developed a vector "toolkit" for S. stercoralis including constructs with the Ss era-1 3′ UTR and each of the promoters described above.

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“…One of the conclusions from these results is that multiple CRs expressed in a given cell type converge onto a common set of downstream signal transduction molecules. For example, a number of sensory neuron-specific Gα proteins appear to act in a complex manner to activate or inhibit signaling upon interaction of any ligand with its cognate CR in a given cell type [19,57,[138][139][140]. These G proteins act via different signaling pathways in different chemosensory neuron types.…”

Section: The Molecules For Taste and Smellmentioning

confidence: 99%

“…However, for cues sensed by both left and right neurons, one mechanism by which the signal transduction pathways could be insulated is via segregation of individual signaling pathways into signaling microdomains [142,[148][149][150][151]. It is also possible that while the core components of the signal transduction pathways are shared, additional molecules act in an odorant pathwayspecific manner [140], allowing the neuron to discriminate between multiple chemical cues. Finally, discrimination may also be effectively achieved by the activation or inhibition of different CRs expressed in a given neuron by different chemicals, or via differential temporal dynamics of CR function [132,133,152].…”

Section: The Molecules For Taste and Smellmentioning

confidence: 99%

Generation and modulation of chemosensory behaviors in C. elegans

Sengupta

2007

Pflugers Arch - Eur J Physiol

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C. elegans recognizes and discriminates among hundreds of chemical cues using a relatively compact chemosensory nervous system. Chemosensory behaviors are also modulated by prior experience and contextual cues. Because of the facile genetics and genomics possible in this organism, C. elegans provides an excellent system in which to explore the generation of chemosensory behaviors from the level of a single gene to the motor output. This review summarizes the current knowledge on the molecular and neuronal substrates of chemosensory behaviors and chemosensory behavioral plasticity in C. elegans.

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The Heterotrimeric G Protein Genes of Caenorhabditis elegans

Cited by 51 publications

References 33 publications

Antagonistic sensory cues generate gustatory plasticity in Caenorhabditis elegans

Antagonistic sensory cues generate gustatory plasticity in Caenorhabditis elegans

Strongyloides stercoralis: Cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3′ UTR

Generation and modulation of chemosensory behaviors in C. elegans

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