The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis

Liu, Dou; Wang, Huiqin; Li, Huaizhu; Xiao, Gao; Zhang, Xianliang

doi:10.3389/fpls.2022.882587

Cited by 9 publications

(11 citation statements)

References 78 publications

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“…The prediction of theoretical core sites for glucose and phosphate binding demonstrated that all homologous HXKs possessed the conserved AA residues (e.g., Asp-101, Gly-104, and Ser-177) except for AtHKL1 and PtHXK4, suggesting that PtHXK4 may be a catalytically inactive glucose sensor ( Figure 4 ). These results were supported by several recent works performed on Cassava, bamboo, and cotton [ 16 , 17 , 18 ]. These conserved features of HXKs may be more variable in gymnosperms, bryophytes, and lycophytes, suggesting that the plant-derived HXKs probably evolved from prokaryotic ancestors, common primordial actin fold protein [ 54 , 55 ].…”

Section: Discussionsupporting

confidence: 85%

“…edulis , Z . mays , A. thaliana , G. raimondii , M. esculenta , N. tobacum , S. lycopersicum , P. patens , and S. moellendorffii were obtained from the literatures [ 1 , 10 , 15 , 16 , 17 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…In rice ( Oryza sativa ), ten HXK isogenes ( OsHXK1 - 10 ) have been isolated, whereas the physiological functions appeared to be diversified owing to playing actions as the positive or negative growth regulator [ 12 , 13 , 14 ]. Moreover, extensive surveys of several HXK families have been reported in Zea Mays , Manihot esculenta , Gossypium hirsutum, Phyllostachys dullish , Physcomitrella patens , and Jatropha curcas , suggesting the HXK conserved patterns in diverse plant species [ 15 , 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

Han

Xiong

et al. 2022

Plants

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Hexokinase (HXK) family proteins exert critical roles in catalyzing hexose phosphorylation, sugar sensing, and modulation of plant growth and stress adaptation. Nevertheless, a large amount remains unknown about the molecular profile of HXK enzymes in Populus trichocarpa, a woody model tree species. A genome-wide survey of HXK-encoding genes, including phylogenies, genomic structures, exon/intron organization, chromosomal distribution, and conserved features, was conducted, identifying six putative HXK isogenes (PtHXK1-6) in the Populus genome. The evolutionary tree demonstrated that 135 homologous HXKs between 17 plant species were categorized into four major subfamilies (type A, B, C, and D), clustering one plastidic (PtHXK3) and five mitochondrial PtHXKs grouped into type A and B, respectively. The in silico deduction prompted the presence of the conserved sugar-binding core (motif 4), phosphorylation sites (motif 2 and 3), and adenosine-binding domains (motif 7). The transcriptomic sequencing (RNA-seq) and the quantitative real-time PCR (qRT-PCR) assays revealed that three isogenes (PtHXK2, 3, and 6) were abundantly expressed in leaves, stems, and roots, while others appeared to be dominantly expressed in the reproductive tissues. Under the stress exposure, PtHXK2 and 6 displayed a significant induction upon the pathogenic fungi (Fusarium solani) infection and marked promotions by glucose feeding in roots. In contrast, the PtHXK3 and 6 are ABA-responsive genes, following a dose-dependent manner. The comprehensive analyses of the genomic patterns and expression profiling provide theoretical clues and lay a foundation for unraveling the physiological and signaling roles underlying the fine-tuned PtHXKs responding to diverse stressors.

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Section: Discussionsupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

Han

Xiong

et al. 2022

Plants

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“…HXKs family proteins play a key regulatory role in sugar sensing, sucrose metabolism, catalyzing hexose phosphorylation, providing energy and modulation of plant growth and stress adaptation [ 27 ]. HXK belongs to a multigene family and HXK is usually encoded by a medium family of approximately 4–10 genes in higher plant, i.e., six members of the HXK family were identified in A. thaliana [ 2 ], while it is represented by 10 members in O. sativa [ 28 ], nine members in Z. mays [ 29 ], nine members in Nicotiana tabacum [ 30 ], 17 members in Gossypium hirsutum L. [ 31 ] and 19 putative members in B. napus [ 32 ] ( Table S5 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression Analysis of Hexokinases Family in Saccharum spontaneum L. under Drought and Cold Stresses

Liu

Jiang

Liu

et al. 2023

Plants

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In plants, the multi-gene family of dual-function hexokinases (HXKs) plays an important role in sugar metabolism and sensing, that affects growth and stress adaptation. Sugarcane is an important sucrose crop and biofuel crop. However, little is known about the HXK gene family in sugarcane. A comprehensive survey of sugarcane HXKs, including physicochemical properties, chromosomal distribution, conserved motifs, and gene structure was conducted, identifying 20 members of the SsHXK gene family that were located on seven of the 32 Saccharum spontaneum L. chromosomes. Phylogenetic analysis showed that the SsHXK family could be divided into three subfamilies (group I, II and III). Motifs and gene structure were related to the classification of SsHXKs. Most SsHXKs contained 8–11 introns which was consistent with other monocots. Duplication event analysis indicated that HXKs in S. spontaneum L. primarily originated from segmental duplication. We also identified putative cis-elements in the SsHXK promoter regions which were involved in phytohormone, light and abiotic stress responses (drought, cold et al.). During normal growth and development, 17 SsHXKs were constitutively expressed in all ten tissues. Among them, SsHXK2, SsHXK12 and SsHXK14 had similar expression patterns and were more highly expressed than other genes at all times. The RNA-seq analysis showed that 14/20 SsHXKs had the highest expression level after cold stress for 6 h, especially SsHXK15, SsHXK16 and SsHXK18. As for drought treatment, 7/20 SsHXKs had the highest expression level after drought stress for 10 days, 3/20 (SsHKX1, SsHKX10 and SsHKX11) had the highest expression level after 10 days of recovery. Overall, our results revealed the potential biological function of SsHXKs, which may provide information for in-depth functional verification.

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“…HXK gene family members have been systematically identified in various plant species. 10, 9, 4, 7, 11, 17 HXK genes members were identified respectively in Oryza sativa (Cho et al, 2006), Zea mays (Zhang et al, 2014), Lycopersicon esculentum (Kandel-Kfir et al, 2006), Manihot esculenta (Geng et al, 2017), Physcomitrella patens (Olsson et al, 2003) and Gossypium hirsutum L (Dou et al, 2022) in their genome.…”

Section: Introductionmentioning

confidence: 99%

Characterization of hexokinase gene family members in Glycine max and functional analysis of GmHXK2 under salt stress

Chen

Tian²,

Guo³

2023

Front. Genet.

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Hexokinase (HXK) is a bifunctional enzyme involved in carbohydrate metabolism and sugar signal sensing. HXK gene family has been extensively discussed in many species, while the detailed investigations of the family in Glycine max have yet to be reported. In this study, 17 GmHXK genes (GmHXKs) were identified in the G. max genome and the features of their encoded proteins, conserved domains, gene structures, and cis-acting elements were systematically characterized. The GmHXK2 gene isolated from G. max was firstly constructed into plant expression vector pMDC83 and then transformed with Agrobacterium tumefaciens into Arabidopsis thaliana. The expression of integrated protein was analyzed by Western Blotting. Subcellular localization analysis showed that the GmHXK2 was located on both vacuolar and cell membrane. Under salt stress, seedlings growth was significantly improved in Arabidopsis overexpressing GmHXK2 gene. Furthermore, physiological indicators and expression of salt stress responsive genes involved in K+ and Na+ homeostasis were significantly lower in GmHXK2-silenced soybean seedlings obtained by virus-induced gene silencing (VIGS) technique under salt stress compared with the control plants. Our study showed that GmHXK2 gene played an important role in resisting salt stress, which suggested potential value for the genetic improvement of abiotic resistant crops.

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The hexokinase Gene Family in Cotton: Genome-Wide Characterization and Bioinformatics Analysis

Cited by 9 publications

References 78 publications

Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

Identification and Expression Analysis of Hexokinases Family in Saccharum spontaneum L. under Drought and Cold Stresses

Characterization of hexokinase gene family members in Glycine max and functional analysis of GmHXK2 under salt stress

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