The Histomorphometry of Liver and Kidney of Hyperglycemic Albino Rats after Treatment with Tithonia diversifolia Leaf Extract

Yuneldi, Rizki Fitrawan; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah

doi:10.15294/biosaintifika.v13i2.29459

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…For histological analysis, the pectoralis muscle and testes were fixed in 10% neutral buffer formalin (NBF) solution for 18–24 h and stained with H and E (Merck, Darmstadt, Germany) [ 24 , 32 , 43 ]. Furthermore, the slides were observed under a microscope (Leica DM 750, Germany) with magnifications of 10 × 10 and 40 × 10.…”

Section: Methodsmentioning

confidence: 99%

Growth, pectoralis muscle performance, and testis of pelung cockerels (Gallus gallus gallus [Linnaeus, 1758]) supplemented with blood clam shell powder (Anadara granosa [Linnaeus, 1758])

et al. 2023

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Background and Aim: Pelung cockerels (Gallus gallus gallusGallus gallus gallus [Linnaeus, 1758]) are different from other native cockerels in that they have a long and unique voice, in addition to their tall, large, and sturdy body with a relatively heavy body weight (BW). The sound quality of pelung cockerels is affected by the structure of the syrinx and their large and strong chest muscles. The performance of the chest muscles, and subsequently its voice, is influenced by the hormone testosterone. The shell of blood clams (Anadara granosa Linnaeus, 1758), a saltwater bivalve is known to contain a natural aromatase blocker (NAB) capable of blocking the aromatase enzyme from converting testosterone to estradiol. This generates consistently high levels of testosterone. This study aimed to determine the effect of blood clam shell powder (BCSP) as an NAB on the growth, pectoralis muscle performance, and testes of pelung cockerels. Materials and Methods: The study design was a completely randomized design, with 16 pelung cockerels aged 40–56 weeks divided into four treatment groups: T0 (control); T1 (BCSP [A. granosa] 0.9 mg/kg BW); T2 (zinc sulfate [ZnSO4] 0.9 mg/kg BW); and T3 (testosterone 3 mg/day). The animals were acclimatized for 7 days and then given dietary treatments for 56 days. The measurement of the comb, wattle, and chest circumference (CC) of pelung cockerels was performed on days 0, 14, 28, 42, and 56. At the end of the treatment, the pelung cockerels were sacrificed and the data of the pectoralis muscle weight (PMW), testis weight (TW), and area of the pectoralis muscle (APM) were measured. Samples of pectoralis muscle and testes were taken and fixed in 10% neutral buffer formalin for histology. The proliferating cell nuclear antigen (PCNA) was identified by immunohistochemical staining. To measure fascicle area (FA), myofiber area (MA), and enumerate, the fascicle myofibers (NM) histology preparations were stained with hematoxylin and eosin (H and E). Testicular preparations were stained with H and E to measure the diameter of the seminiferous tubules (DST) using ImageJ software. Results: The growth performance on day 56 showed significantly (p < 0.05) higher differences of CC in T1 compared to T2 and T0, in T1 and T3 compared to T0, and in T3 and T2 compared to T0. Pectoralis muscle results, that is, FA, NM, MA, and PCNA-positive cells, showed that cockerels on treatment T3 had significantly higher results than other treatments, T1 was significantly different from T2 and T0, and T2 was significantly different from T0. In addition, the TW and DST measurement of cockerels on treatment T3 were significantly reduced (p < 0.05) than the other treatment groups. Conclusion: The oral administration of BCSP in the role of a NAB at a dose of 0.9 mg/kg BW for 56 days improved the growth performance and pectoralis muscle, especially the CC, FA, NM, MA, and PCNA-positive cells parameters, but did not affect the PMW, APM, and testis of pelung cockerels. The administration of testosterone at 3 mg/day for 56 days contributed to the decrease in TW and DST, as well as atrophy of the seminiferous tubules of pelung cockerels. Keywords: growth performance, muscle, natural aromatase blocker, pelung, testis.

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Section: Methodsmentioning

confidence: 99%

Growth, pectoralis muscle performance, and testis of pelung cockerels (Gallus gallus gallus [Linnaeus, 1758]) supplemented with blood clam shell powder (Anadara granosa [Linnaeus, 1758])

et al. 2023

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The Effect of Natural Aromatase Blocker on the Growth Comb and Body Weight of Layer Chicken

et al. 2022

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The aim of this research is to evaluate the effect of Anadara granosa shell powder as a natural aromatase blocker (NAB) and zinc sulfate (ZnSO4) on the growth of the length comb, width comb, and body weight (BW) of layer chicken. This study used 45 DOC of layer’s chickens with 3 treatments and 15 replications, those are; control (T0), clamshell powder Anadara granosa as NAB 0.036 mg/40 g BW (T1), and ZnSO4 0.018 mg / 40 g BW (T2). This treatment was given for 35 days. The data on the growth of length comb, width comb, and BW are measured weekly. The data collected was analyzed using one-way ANOVA at the 95% confidence level with the assistance of SPSS. The results of statistical analysis of the length and width of the comb in the same column showed that T0 was not significantly different (p>0.05) from other treatments. The statistical analysis shows that the length and width of the layer’s chicken comb in the same row at T0, T1 and T2 were significantly different (p<0.05). The results of statistical analysis of the BW in the same column showed that T1 was significantly different (p<0.05) from other treatments. It can be concluded that additional NAB 0.036 mg/40 g BW can increase the body weight of male layer chicken after treatment of 35 days. The administration of NAB and ZnSO4 could not increase the growth of the length and width of layer chicken combs. The growth in length and width of layer chicken combs is very fast in the starter phase.

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The Histomorphometry of Liver and Kidney of Hyperglycemic Albino Rats after Treatment with Tithonia diversifolia Leaf Extract

Cited by 2 publications

References 26 publications

Growth, pectoralis muscle performance, and testis of pelung cockerels (Gallus gallus gallus [Linnaeus, 1758]) supplemented with blood clam shell powder (Anadara granosa [Linnaeus, 1758])

Growth, pectoralis muscle performance, and testis of pelung cockerels (Gallus gallus gallus [Linnaeus, 1758]) supplemented with blood clam shell powder (Anadara granosa [Linnaeus, 1758])

The Effect of Natural Aromatase Blocker on the Growth Comb and Body Weight of Layer Chicken

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