The Hsp90/Cdc37p chaperone system is a determinant of molybdate resistance in <i>Saccharomyces cerevisiae</i>

Millson, Stefan H.; Nuttall, James; Mollapour, Mehdi; Piper, Peter W.

doi:10.1002/yea.1670

Cited by 9 publications

(6 citation statements)

References 46 publications

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“…In fact, high molybdate levels are inhibitory for this organism. Recently, an efficient Hsp90/Cdc37p chaperone system was identified as a determinant of molybdate resistance in S. cerevisiae (82 (56). In our work, rice (O. sativa) appeared to contain even more Cu-dependent proteins than A. thaliana.…”

Section: Resultsmentioning

confidence: 59%

General Trends in Trace Element Utilization Revealed by Comparative Genomic Analyses of Co, Cu, Mo, Ni, and Se

Zhang

Gladyshev

2010

Journal of Biological Chemistry

111

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Trace elements are used by all organisms and provide proteins with unique coordination and catalytic and electron transfer properties. Although many trace element-containing proteins are well characterized, little is known about the general trends in trace element utilization. We carried out comparative genomic analyses of copper, molybdenum, nickel, cobalt (in the form of vitamin B 12 ), and selenium (in the form of selenocysteine) in 747 sequenced organisms at the following levels: (i) transporters and transport-related proteins, (ii) cofactor biosynthesis traits, and (iii) trace element-dependent proteins. Few organisms were found to utilize all five trace elements, whereas many symbionts, parasites, and yeasts used only one or none of these elements. Investigation of metalloproteomes and selenoproteomes revealed examples of increased utilization of proteins that use copper in land plants, cobalt in Dehalococcoides and Dictyostelium, and selenium in fish and algae, whereas nematodes were found to have great diversity of copper transporters. These analyses also characterized trace element metabolism in common model organisms and suggested new model organisms for experimental studies of individual trace elements. Mismatches in the occurrence of user proteins and corresponding transport systems revealed deficiencies in our understanding of trace element biology. Biological interactions among some trace elements were observed; however, such links were limited, and trace elements generally had unique utilization patterns. Finally, environmental factors, such as oxygen requirement and habitat, correlated with the utilization of certain trace elements. These data provide insights into the general features of utilization and evolution of trace elements in the three domains of life.Biological trace elements refer to chemical elements required in minute quantities by an organism (1-3) and include chromium, cobalt, copper, iodine, iron, manganese, molybdenum, nickel, selenium, tungsten, vanadium, zinc, and probably several other elements. These trace elements function in different ways. Some are essential components of enzymes where they directly interact with substrates and often facilitate their conversion to products; some donate or accept electrons in reactions of reduction and oxidation; some structurally stabilize biological molecules; and some control biological processes by facilitating the binding of molecules to receptor sites on cell membranes (4).Most biological trace elements are metals. Among them, Fe and Zn are thought to be the most abundant transition metal ions that are used by all organisms (5, 6). Other metals, such as Mn, Cu, Mo, Ni, and Co, are utilized by various metalloproteins in a wide range of organisms in all three domains of life. Additionally, Se, the major metalloid micronutrient, plays roles in various redox and metabolic processes (7-9).The ability of the cell to maintain a specific trace element within a certain homeostatic range is mainly dependent on the processes of uptake, storage, ...

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Section: Resultsmentioning

confidence: 59%

General Trends in Trace Element Utilization Revealed by Comparative Genomic Analyses of Co, Cu, Mo, Ni, and Se

Zhang

Gladyshev

2010

Journal of Biological Chemistry

111

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“…Most likely the loss of the two Mo-containing enzymes and the Mo-cofactor biosynthesis is the outcome of biotrophy and not the reason for biotrophy, though conceivably there may have been selection against this pathway if other nitrogen or sulphate sources are less energy-consuming and therefore enhance fitness during parasitism. Molybdenum has been reported to interfere with function of chaperones like Hsp90 [44],[45]. Avoiding the uptake of molybdenum might prevent this Hsp90 inhibition and increase fitness on Ar.…”

Section: Resultsmentioning

confidence: 99%

Gene Gain and Loss during Evolution of Obligate Parasitism in the White Rust Pathogen of Arabidopsis thaliana

et al. 2011

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Biotrophic eukaryotic plant pathogens require a living host for their growth and form an intimate haustorial interface with parasitized cells. Evolution to biotrophy occurred independently in fungal rusts and powdery mildews, and in oomycete white rusts and downy mildews. Biotroph evolution and molecular mechanisms of biotrophy are poorly understood. It has been proposed, but not shown, that obligate biotrophy results from (i) reduced selection for maintenance of biosynthetic pathways and (ii) gain of mechanisms to evade host recognition or suppress host defence. Here we use Illumina sequencing to define the genome, transcriptome, and gene models for the obligate biotroph oomycete and Arabidopsis parasite, Albugo laibachii. A. laibachii is a member of the Chromalveolata, which incorporates Heterokonts (containing the oomycetes), Apicomplexa (which includes human parasites like Plasmodium falciparum and Toxoplasma gondii), and four other taxa. From comparisons with other oomycete plant pathogens and other chromalveolates, we reveal independent loss of molybdenum-cofactor-requiring enzymes in downy mildews, white rusts, and the malaria parasite P. falciparum. Biotrophy also requires “effectors” to suppress host defence; we reveal RXLR and Crinkler effectors shared with other oomycetes, and also discover and verify a novel class of effectors, the “CHXCs”, by showing effector delivery and effector functionality. Our findings suggest that evolution to progressively more intimate association between host and parasite results in reduced selection for retention of certain biosynthetic pathways, and particularly reduced selection for retention of molybdopterin-requiring biosynthetic pathways. These mechanisms are not only relevant to plant pathogenic oomycetes but also to human pathogens within the Chromalveolata.

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“…Many Hsp90 co-chaperones are conserved in all eukaryotes. For this reason, the single cell eukaryote baker's yeast, Saccharomyces cerevisiae , has proven to be an excellent model organism to study the function of both Hsp90 and its co-chaperones in maintaining cellular homeostatis [46,47,48,49,50,51]. …”

Section: Post-translational Modification Of Hsp90 Co-chaperonesmentioning

confidence: 99%

Post-translational modifications of Hsp90 and their contributions to chaperone regulation

Mollapour

Neckers

2012

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

Self Cite

266

212

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Molecular chaperones, as the name suggests, are involved in folding, maintenance, intracellular transport, and degradation of proteins as well as in facilitating cell signaling. Heat shock protein 90 (Hsp90) is an essential eukaryotic molecular chaperone that carries out these processes in normal and cancer cells. Hsp90 function in vivo is coupled to its ability to hydrolyze ATP and this can be regulated by co-chaperones and post-translational modifications. In this review, we explore the varied roles of known post-translational modifications of cytosolic and nuclear Hsp90 (phosphorylation, acetylation, S-nitrosylation, oxidation and ubiquitination) in fine-tuning chaperone function in eukaryotes.

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The Hsp90/Cdc37p chaperone system is a determinant of molybdate resistance in Saccharomyces cerevisiae

Cited by 9 publications

References 46 publications

General Trends in Trace Element Utilization Revealed by Comparative Genomic Analyses of Co, Cu, Mo, Ni, and Se

General Trends in Trace Element Utilization Revealed by Comparative Genomic Analyses of Co, Cu, Mo, Ni, and Se

Gene Gain and Loss during Evolution of Obligate Parasitism in the White Rust Pathogen of Arabidopsis thaliana

Post-translational modifications of Hsp90 and their contributions to chaperone regulation

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