The human micro‐vascular endothelial cells in vitro interaction with atomic‐nitrogen‐doped diamond‐like carbon thin films

Abstract

This paper reports the initial response of atomic nitrogen doped diamond like carbon (DLC) to endothelial cells in vitro. The introduction of nitrogen atoms/molecules to the diamond like carbon structures leads to an atomic structural change favorable to the attachment of human micro-vascular endothelial cells. Whilst the semi-conductivity induced by nitrogen in DLC is thought to play a part, the increase in the non-bonded N atoms and N(2) molecules in the atomic doped species (with the exclusion of the charged species) seems to contribute to the improved attachment of human microvascular endothelial cells. The increased endothelial attachment is associated with a lower work function and slightly higher water contact angle in the atomic doped films, where the heavy charged particles are excluded. The films used in the study were synthesized by the RF PECVD technique followed by post deposition doping with nitrogen, and afterwards the films were characterized by XPS, Raman spectroscopy, SIMS and Kelvin probe. The water contact angles were measured, and the counts of the adherent endothelial cells on the samples were carried out. This study is relevant and contributory to improving biocompatibility of surgical implants and prostheses.

Diamond-Like Carbon Coatings for Joint Arthroplasty

“…Several attempts have been made to further improve the properties of DLC. The amorphous nature of DLC allows for incorporation of a third element, such as Si (Jones et al, 2010;Lee et al, 1997Lee et al, , 2002Maguire et al, 2005;Ogwu et al, 2008;Okpalugo et al, 2004;Papakonstantinou et al, 2002), F (Hasebe et al, 2006(Hasebe et al, , 2007Saito et al, 2005), P (Kwok et al, 2005), Ag (Ahmed et al, 2009;Choi et al, 2007Choi et al, , 2008Kwok et al, 2007), N (Okpalugo et al, 2008;Yokota et al, 2007), or Ti (Bui et al, 2008;Gilmore and Hauert, 2001;Ouyang and Sasaki, 2005;Pandiyaraj et al, 2012), the concentration of which significantly affects the resulting DLC properties. For example , Choi et al (2007) prepared Ag-incorporated DLC films using ion beam…”

The effect of amino plasma‐enhanced chemical vapor deposition‐treated titanium surface on Schwann cells

“…Plasma‐enhanced chemical vapor deposition (PECVD) is one of these surface modification techniques. After modification with PEVCD, the surface of Ti implants may show better biocompatibility or bioactivity, depending on the characteristics of the polymers deposed . A previous study performed surface modification of Ti disks using PEVCD with ammonia plasma and reported that amino group PECVD functionalization was nontoxic for the osteoblast cell line and fibroblast cell line and can promote cell adhesion and osseointegration in vitro .…”

Human Umbilical Vein Endothelial Cell Interaction with Fluorine-Incorporated Amorphous Carbon Films

“…22) Nitrogen-incorporated a-C:H (a-C:H:N) is also reported as well biocompatible thin films with ECs. 23,24) In our present study, we have focused on the effects of fluorine incorporation upon HUVEC adhesion and proliferation. We assessed the viability of HUVECs by measuring the enzymatic activity using a Cell Counting Kit-8 (WST-8 assay kit).…”