The<i>REDUCED LEAFLET</i>Genes Encode Key Components of the trans-Acting Small Interfering RNA Pathway and Regulate Compound Leaf and Flower Development in<i>Lotus japonicus</i>

Yan, Jun; Cai, Xue-Fei; Luo, Jun; Sato, Shusei; Jiang, Qunyi; Yang, Jun; Xiao, Cao; Xiu, Hu; Tabata, Satoshi; Gresshoff, Peter M.; Luo, Da

doi:10.1104/pp.109.140947

Cited by 46 publications

(59 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Arabidopsis, the juvenile-toadult phase transition is normally suppressed by TAS3a tasiRNAs through the negative regulation of ARF3 mRNA (Fahlgren et al, 2006). Late stages of flower development in Arabidopsis, rice, and Lotus japonicus rely on regulated ARF3 function (Hunter et al, 2006;Liu et al, 2007;Yan et al, 2010). Furthermore, recent data indicate that tasiRNAs are also involved in environmental stress responses (Moldovan et al, 2010).…”

Section: Mirnas/tasirna-arf Responses To Cold Stress In Spikes Of Thementioning

confidence: 99%

Uncovering Small RNA-Mediated Responses to Cold Stress in a Wheat Thermosensitive Genic Male-Sterile Line by Deep Sequencing

Tang

Zhang²,

Xu³

et al. 2012

Plant Physiology

160

135

View full text Add to dashboard Cite

The male sterility of thermosensitive genic male sterile (TGMS) lines of wheat (Triticum aestivum) is strictly controlled by temperature. The early phase of anther development is especially susceptible to cold stress. MicroRNAs (miRNAs) play an important role in plant development and in responses to environmental stress. In this study, deep sequencing of small RNA (smRNA) libraries obtained from spike tissues of the TGMS line under cold and control conditions identified a total of 78 unique miRNA sequences from 30 families and trans-acting small interfering RNAs (tasiRNAs) derived from two TAS3 genes. To identify smRNA targets in the wheat TGMS line, we applied the degradome sequencing method, which globally and directly identifies the remnants of smRNA-directed target cleavage. We identified 26 targets of 16 miRNA families and three targets of tasiRNAs. Comparing smRNA sequencing data sets and TaqMan quantitative polymerase chain reaction results, we identified six miRNAs and one tasiRNA (tasiRNA-ARF [for Auxin-Responsive Factor]) as cold stress-responsive smRNAs in spike tissues of the TGMS line. We also determined the expression profiles of target genes that encode transcription factors in response to cold stress. Interestingly, the expression of cold stress-responsive smRNAs integrated in the auxin-signaling pathway and their target genes was largely noncorrelated. We investigated the tissue-specific expression of smRNAs using a tissue microarray approach. Our data indicated that miR167 and tasiRNA-ARF play roles in regulating the auxin-signaling pathway and possibly in the developmental response to cold stress. These data provide evidence that smRNA regulatory pathways are linked with male sterility in the TGMS line during cold stress.

show abstract

Section: Mirnas/tasirna-arf Responses To Cold Stress In Spikes Of Thementioning

confidence: 99%

Uncovering Small RNA-Mediated Responses to Cold Stress in a Wheat Thermosensitive Genic Male-Sterile Line by Deep Sequencing

Tang

Zhang²,

Xu³

et al. 2012

Plant Physiology

160

135

View full text Add to dashboard Cite

show abstract

“…Tomato (Solanum lycopersicum) WIRY genes are involved in ta-siRNA biogenesis, and the wiry mutants show shoestring leaves with defects in leaf blade expansion (Yifhar et al, 2012). In the legume species Lotus japonicus, mutations in REDUCED LEAFLET1 (REL1) and RE-DUCED LEAFLET3 (REL3), which respectively encode the orthologs of Arabidopsis SGS3 and AGO7, result in abaxialized leaflets with decreased leaflet numbers (Yan et al, 2010). In monocots, more obvious developmental defects have been observed in ta-siRNA pathway-related mutants.…”

Section: Introductionmentioning

confidence: 99%

“…Independent studies have shown that the ta-siRNA pathway is broadly involved in the determination of dorsiventral polarity of leaves among species (Timmermans et al, 1998;Juarez et al, 2004;Liu et al, 2007;Yan et al, 2010). Therefore, the ta-siRNA pathway probably plays a key role in leaf morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

TheTrans-Acting Short Interfering RNA3 Pathway and NO APICAL MERISTEM Antagonistically Regulate Leaf Margin Development and Lateral Organ Separation, as Revealed by Analysis of anargonaute7/lobed leaflet1Mutant inMedicago truncatula

Zhou

Han

et al. 2013

The Plant Cell

View full text Add to dashboard Cite

Leaf shape elaboration and organ separation are critical for plant morphogenesis. We characterized the developmental roles of LOBED LEAFLET1 by analyzing a recessive mutant in the model legume Medicago truncatula. An ortholog of Arabidopsis thaliana ARGONAUTE7 (AGO7), Mt-AGO7/LOBED LEAFLET1, is required for the biogenesis of a trans-acting short interfering RNA (ta-siRNA) to negatively regulate the expression of AUXIN RESPONSE FACTORs in M. truncatula. Loss of function in AGO7 results in pleiotropic phenotypes in different organs. The prominent phenotype of the ago7 mutant is lobed leaf margins and more widely spaced lateral organs, suggesting that the trans-acting siRNA3 (TAS3) pathway negatively regulates the formation of boundaries and the separation of lateral organs in M. truncatula. Genetic interaction analysis with the smooth leaf margin1 (slm1) mutant revealed that leaf margin formation is cooperatively regulated by the auxin/SLM1 (ortholog of Arabidopsis PIN-FORMED1) module, which influences the initiation of leaf margin teeth, and the TAS3 ta-siRNA pathway, which determines the degree of margin indentation. Further investigations showed that the TAS3 ta-siRNA pathway and NO APICAL MERISTEM (ortholog of Arabidopsis CUP-SHAPED COTYLEDON) antagonistically regulate both leaf margin development and lateral organ separation, and the regulation is partially dependent on the auxin/SLM1 module.

show abstract

“…In contrast to the essential role of this pathway in maize and rice development (Nogueira et al, 2007;Nagasaki et al, 2007;Itoh et al, 2008;Dotto et al, 2014), ta-siRNA biogenesis mutants in the eudicots exhibit relatively subtle defects. In Arabidopsis, Medicago, and Lotus, such mutants give rise to downward-curled, lobed, or narrow leaves that are weakly abaxialized (Peragine et al, 2004;Xie et al, 2005;Yan et al, 2010;Zhou et al, 2013). Even in tomato, where ta-siRNA biogenesis mutants show a more severe effect on leaf polarity, these mutants are viable and male fertile (Yifhar et al, 2012;Brooks et al, 2014).…”

Section: Discussion Tasir-arf-directed Regulation Of Arf3 Is Essentiamentioning

confidence: 99%

“…Disruption of this pathway in other eudicot species similarly results in viable plants with partially abaxialized or highly lobed leaves (Yan et al, 2010;Zhou et al, 2013). Even in tomato (Solanum lycopersicum), where the effects of ta-siRNA biogenesis mutants on leaf polarity are more exacerbated, such mutants develop to maturity and remain partially fertile (Yifhar et al, 2012;Brooks et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Novel DICER-LIKE1 siRNAs Bypass the Requirement for DICER-LIKE4 in Maize Development

et al. 2015

View full text Add to dashboard Cite

Dicer enzymes function at the core of RNA silencing to defend against exogenous RNA or to regulate endogenous genes. Plant DICER-LIKE4 (DCL4) performs dual functions, acting in antiviral defense and in development via the biogenesis of transacting short-interfering RNAs (siRNAs) termed tasiR-ARFs. These small RNAs play an essential role in the grasses, spatially defining the expression domain of AUXIN RESPONSE FACTOR3 (ARF3) transcription factors. However, contrary to tasiRARFs' essential function in development, DCL4 proteins exhibit strong evidence of recurrent adaptation typical of host factors involved in antiviral immunity. Here, we address how DCL4 balances its role in development with pressures to diversify in response to viral attack. We show that, in contrast to other tasiR-ARF biogenesis mutants, dcl4 null alleles have an uncharacteristically mild phenotype, correlated with normal expression of select arf3 targets. Loss of DCL4 activity yields a class of 22-nucleotide tasiR-ARF variants associated with the processing of arf3 transcripts into 22-nucleotide secondary siRNAs by DCL1. Our findings reveal a DCL1-dependent siRNA pathway that bypasses the otherwise adverse developmental effects of mutations in DCL4. This pathway is predicted to have important implications for DCL4's role in antiviral defense by reducing the selective constraints on DCL4 and allowing it to diversify in response to viral suppressors.

show abstract

TheREDUCED LEAFLETGenes Encode Key Components of the trans-Acting Small Interfering RNA Pathway and Regulate Compound Leaf and Flower Development inLotus japonicus

Cited by 46 publications

References 56 publications

Uncovering Small RNA-Mediated Responses to Cold Stress in a Wheat Thermosensitive Genic Male-Sterile Line by Deep Sequencing

Uncovering Small RNA-Mediated Responses to Cold Stress in a Wheat Thermosensitive Genic Male-Sterile Line by Deep Sequencing

TheTrans-Acting Short Interfering RNA3 Pathway and NO APICAL MERISTEM Antagonistically Regulate Leaf Margin Development and Lateral Organ Separation, as Revealed by Analysis of anargonaute7/lobed leaflet1Mutant inMedicago truncatula

Novel DICER-LIKE1 siRNAs Bypass the Requirement for DICER-LIKE4 in Maize Development

Contact Info

Product

Resources

About