The <i>Terminalia laxiflora</i> modulates the neurotoxicity induced by fipronil in male albino rats

Khalaf, A.A.; Galal, Mona K.; Ibrahim, Marwa A.; Allah, A.A. Abd; Afify, Mostafa M.; Refaat, Rasha

doi:10.1042/bsr20181363

Cited by 14 publications

(14 citation statements)

References 64 publications

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“…It also altered the 5-HT tissue content. Previous studies showed that FPN dose-dependently changed the activity of various enzymes involved in oxidative stress and inflammation in the brain when chronically administered at the 5–30 mg/kg range of doses [ 8 , 9 , 11 , 12 , 38 ]. Thus, the dose of 10 mg/kg, considered as a moderate dose, profoundly alters the neurochemistry of monoamines, particularly DA and 5-HT.…”

Section: Discussionmentioning

confidence: 99%

“…The earlier reports suggest that FPN blocks chloride ion cellular uptake in invertebrates, leading to uncontrolled central nervous system (CNS) hyper-excitation by the blockage of GABA A receptors, convulsion, and cell death [ 6 , 7 ]. Although considered less toxic toward mammals, its absorption is rapid, and it easily crosses the blood brain barrier [ 8 ]. Recent alarming reports suggest that chronic FPN in rodents at 30 but not 10 mg/kg by daily oral gavage causes memory impairment in parts associated with the modulation of the GABAergic system [ 9 ] and the deposition of amyloid plaques [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recent alarming reports suggest that chronic FPN in rodents at 30 but not 10 mg/kg by daily oral gavage causes memory impairment in parts associated with the modulation of the GABAergic system [ 9 ] and the deposition of amyloid plaques [ 10 ]. FPN oral administration raises the oxidative stress in various tissues, including the adipose tissue, the liver, and the brain, in a range of doses of 5–30 mg/kg [ 8 , 11 , 12 , 13 ]. Upon intra-nigral administration in rats, FPN reduced the dopamine (DA) content in the striatum as well as the tyrosine hydroxylase (TH) levels in the striatum and the substantia nigra (SN) pars compacta, and impaired motor coordination [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Chronic Administration of Fipronil Heterogeneously Alters the Neurochemistry of Monoaminergic Systems in the Rat Brain

Bharatiya

Chagraoui

Deurwaerdere

et al. 2020

IJMS

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Fipronil (FPN), a widely used pesticide for agricultural and non-agricultural pest control, is possibly neurotoxic for mammals. Brain monoaminergic systems, involved in virtually all brain functions, have been shown to be sensitive to numerous pesticides. Here, we addressed the hypothesis that chronic exposure to FPN could modify brain monoamine neurochemistry. FPN (10 mg/kg) was chronically administered for 21 days through oral gavage in rats. Thereafter, the tissue concentrations of dopamine (DA) and its metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid; serotonin (5-HT) and its metabolite, 5-hydroxyindoleacetic acid (5-HIAA); and noradrenaline (NA) were measured in 30 distinct brain regions. FPN significantly decreased DA and its metabolite levels in most striatal territories, including the nucleus accumbens and the substantia nigra (SN). FPN also diminished 5-HT levels in some striatal regions and the SN. The indirect index of the turnovers, DOPAC/DA and 5-HIAA/5-HT ratios, was increased in numerous brain regions. FPN reduced the NA content only in the nucleus accumbens core. Using the Bravais–Pearson test to study the neurochemical organization of monoamines through multiple correlative analyses across the brain, we found fewer correlations for NA, DOPAC/DA, and 5-HIAA/5-HT ratios, and an altered pattern of correlations within and between monoamine systems. We therefore conclude that the chronic administration of FPN in rats induces massive and inhomogeneous changes in the DA and 5-HT systems in the brain.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chronic Administration of Fipronil Heterogeneously Alters the Neurochemistry of Monoaminergic Systems in the Rat Brain

Bharatiya

Chagraoui

Deurwaerdere

et al. 2020

IJMS

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“…Each sample was homogenised using liquid nitrogen, and the total RNA was extracted using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions. Both the concentration and purity of the isolated RNA were tested by NanoDrop . The RT‐PCR was performed using the RevertAid First Strand cDNA Synthesis Kit according to the guidelines.…”

Section: Methodsmentioning

confidence: 99%

Histopathological, immunohistochemical, and molecular studies for determination of wound age and vitality in rats

Khalaf

Hassanen

Zaki

et al. 2019

International Wound Journal

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In forensic medicine, it is vital to verify with the best attainable accuracy once injuries occurred during vital or post‐mortem conditions. An immunohistochemical study was carried out to examine the time‐dependent expression of macrophage‐specific gene CD68 (cluster of differentiation 68), alpha‐smooth muscle actin (α‐SMA), and vascular endothelial growth factor (VEGF) in different skin wound timings (0, 1, 3, 5, 7, and 14 days) in rats. Histopathological studies were performed to assess the wound age and vitality. Eighteen male albino Wister rats (weighing 170‐200 g) were used for wound induction. Rats (n = 3) were euthanised at 0, 1, 3, 5, 7, and 14 days from the starting point of wound induction. Histopathological examination showed that the epidermal re‐epithelialisation was completed 14 days after skin incision. The inflammatory phase was recorded during the first 3 days of healing and reached the maximum levels at 5 days, then declined after 7 days, and completely removed at 14 days. The beginning of the proliferative phase was dated to day 3 and the peak at days 5 and 7. The initiation of the granulation tissue formation and remodelling phase of the healing process was observed 5 days after wounding. By immunohistochemical staining, negative VEGF gene expressions at early stages (0‐3 days) were observed, as well as neither CD68+ macrophages nor α‐SMA+ myofibroblast cells were detected. By increasing the wound ages (5‐7 days), granulation tissue and angiogenesis were observed, with the migration of macrophages and fibroblast, which expressed VEGF, CD68, and α‐SMA positive reaction. Time‐dependent expression of the above markers suggested that they would be useful indicators for the determination of wound age. Both VEGF and transforming growth factor‐beta 1 (TGFb1) mRNA levels were determined in different skin wound ages. The transcription of TGFb1 and VEGF increased shortly after wounding, until post‐wounding day 7. It then declined constantly, reaching minimal values on day 14.

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“…After the concentration and purity of RNA were confirmed, 1000 ng purified RNA was used to synthesize 20 μl cDNA, using a Revert aid™ first-strand cDNA synthesis kit (Fermentas, Germany) according to the manufacturer's instructions (Khalaf et al 2019a). Relative quantitative assessment of mRNA transcript amplification for each gene was performed relative to betaactin (ACTB) by a fluorescence-based real-time detection method with a fluorescent SYBR Green dye (Thermo Scientific) (Khalaf et al 2019b). The sequences of all the primer sets used are listed in Table 1.…”

Section: Assessment Of Liver Function and Antioxidant Activitymentioning

confidence: 99%

Innovative application of helium-neon laser: enhancing the germination of Adansonia digitata and evaluating the hepatoprotective activities in mice

Khamis

Hassan

Morsy

et al. 2020

Environ Sci Pollut Res

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The laser pretreatment of seed is drawing pronounced attention from the scientific community for its positive impact in boosting germination, seedling, and growth of plants. In this study, the laser pretreatment of Adansonia digitata (A. digitata) seeds was evaluated. Eight laser treatments were conducted at different powers, 10, 20, 40, and 80 mW, with the two-time interval for each power at 2 and 4 min. The outcomes indicated that the most efficient irradiation was 10 mW/2 min which induces the highest germination rate and polyphenolic contents for seeds. Based on these results, the animal experimental design was processed to assess the hepatoprotective activity of A. digitata extracts obtained through the optimum laser preillumination to enhance the resistance of liver damage in mice. The total phenol and flavonoid contents and the antioxidant properties of the methanolic extracts were estimated in vitro. The CCl4 was used to induce hepatotoxicity in mice. The animals were divided into five groups. The sera of the treated animals were used for the determination of transaminases, and the liver homogenates were used for the determination of antioxidant status, and further liver tissues were subjected to verify the anti-apoptotic effect of A. digitata methanolic extract. The in vivo results showed that the methanolic extract exposed to laser treatment at 10 mW/2 min provided better hepatoprotective capacity than the other treatments. Administration of A. digitata extract not only offered a significant decrease in liver enzyme activity but also markedly improved the antioxidant status and reduced the apoptotic progression induced by CCl4 toxicity in liver tissue.

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The Terminalia laxiflora modulates the neurotoxicity induced by fipronil in male albino rats

Cited by 14 publications

References 64 publications

Chronic Administration of Fipronil Heterogeneously Alters the Neurochemistry of Monoaminergic Systems in the Rat Brain

Chronic Administration of Fipronil Heterogeneously Alters the Neurochemistry of Monoaminergic Systems in the Rat Brain

Histopathological, immunohistochemical, and molecular studies for determination of wound age and vitality in rats

Innovative application of helium-neon laser: enhancing the germination of Adansonia digitata and evaluating the hepatoprotective activities in mice

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