The climactic Los Chocoyos (LCY) eruption from Atitlán caldera (Guatemala) is a key chronostratigraphic marker for the Quaternary period given the extensive distribution of its deposits that reached both the Pacific and Atlantic Oceans. Despite LCY tephra being an important marker horizon, a radioisotopic age for this eruption has remained elusive. Using zircon (U-Th)/He geochronology, we present the first radioisotopically determined eruption age for the LCY of 75 ± 2 ka. Additionally, the youngest zircon crystallization 238 U-230 Th rim ages in their respective samples constrain eruption age maxima for two other tephra units that erupted from Atitlán caldera, W-Fall (130 +16 / −14 ka) and I-Fall eruptions (56 +8.2 / −7.7 ka), which under-and overlie LCY tephra, respectively. Moreover, rim and interior zircon dating and glass chemistry suggest that before eruption silicic magma was stored for >80 kyr, with magma accumulation peaking within ca. 35 kyr before the LCY eruption during which the system may have developed into a vertically zoned magma chamber. Based on an updated distribution of LCY pyroclastic deposits, a new conservatively estimated volume of~1220 ± 150 km 3 is obtained (volcanic explosivity index VEI > 8), which confirms the LCY eruption as the first-ever recognized supereruption in Central America.