The Impact of Proteomics on Products and Processes

Müllner, Stefan

doi:10.1007/3-540-36459-5_1

Cited by 4 publications

(1 citation statement)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As research interest in biotechnology is beginning to shift from genomics to proteomics, the number of recombinant proteins to be expressed is rapidly increasing (Pandey and Mann, 2000;Patterson, 2000;Service, 2001;Kaiser, 2002;Gerstein et al, 2002;Tyers and Mann, 2003;Oliver, 2003;Mullner, 2003). The current total worldwide capacity for recombinant proteins production is estimated to be about 400,000 L, whereas about 5-6 times this capacity will be needed to satisfy the market demands before the end of the present decade (Miller and Nagarajan, 2000;Andersson and Mynahan, 2001;Gura, 2002).…”

Section: Introductionmentioning

confidence: 99%

Perfusion Culture of Hybridoma Cells for Hyperproduction of IgG_2a Monoclonal Antibody in a Wave Bioreactor‐Perfusion Culture System

Tang

Ohashi

Hamel

2007

Biotechnology Progress

View full text Add to dashboard Cite

A novel wave bioreactor-perfusion culture system was developed for highly efficient production of monoclonal antibody IgG2a (mAb) by hybridoma cells. The system consists of a wave bioreactor, a floating membrane cell-retention filter, and a weight-based perfusion controller. A polyethylene membrane filter with a pore size of 7 microm was floating on the surface of the culture broth for cell retention, eliminating the need for traditional pump around flow loops and external cell separators. A weight-based perfusion controller was designed to balance the medium renewal rate and the harvest rate during perfusion culture. BD Cell mAb Medium (BD Biosciences, CA) was identified to be the optimal basal medium for mAb production during batch culture. A control strategy for perfusion rate (volume of fresh medium/working volume of reactor/day, vvd) was identified as a key factor affecting cell growth and mAb accumulation during perfusion culture, and the optimal control strategy was increasing perfusion rate by 0.15 vvd per day. Average specific mAb production rate was linearly corrected with increasing perfusion rate within the range of investigation. The maximum viable cell density reached 22.3 x 105 and 200.5 x 105 cells/mL in the batch and perfusion culture, respectively, while the corresponding maximum mAb concentration reached 182.4 and 463.6 mg/L and the corresponding maximum total mAb amount was 182.4 and 1406.5 mg, respectively. Not only the yield of viable cell per liter of medium (32.9 x 105 cells/mL per liter medium) and the mAb yield per liter of medium (230.6 mg/L medium) but also the mAb volumetric productivity (33.1 mg/L.day) in perfusion culture were much higher than those (i.e., 22.3 x 105 cells/mL per liter medium, 182.4 mg/L medium, and 20.3 mg/L.day) in batch culture. Relatively fast cell growth and the perfusion culture approach warrant that high biomass and mAb productivity may be obtained in such a novel perfusion culture system (1 L working volume), which offers an alternative approach for producing gram quantity of proteins from industrial cell lines in a liter-size cell culture. The fundamental information obtained in this study may be useful for perfusion culture of hybridoma cells on a large scale.

show abstract

Section: Introductionmentioning

confidence: 99%

Perfusion Culture of Hybridoma Cells for Hyperproduction of IgG_2a Monoclonal Antibody in a Wave Bioreactor‐Perfusion Culture System

Tang

Ohashi

Hamel

2007

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

Protein Immobilization Using Atmospheric‐Pressure Dielectric‐Barrier Discharges: A Route to a Straightforward Manufacture of Bioactive Films

Heyse

Roeffaers

Paulussen

et al. 2008

Plasma Processes & Polymers

View full text Add to dashboard Cite

An alternative single‐step method for biomolecule entrapment in atmospheric plasma polymers is described. Relying on our experience to fabricate organic coatings at mild plasma conditions, fluorescent proteins and an organic coating precursor were simultaneously introduced to the DBD. Fluorescence microscopy and spectrum analysis unambiguously illustrate the single‐step protein immobilization procedure using DBD. Moreover, in contrast to covalent immobilization procedures, the developed technique showed a homogeneous protein distribution in the coating.

show abstract

Application of Genomic and Proteomic Data for Bioprocess Analysis and Optimization

Zeng¹,

Sun²,

Wangb³

et al. 2007

Bioprocessing for Value-Added Products From Renewable Resources

View full text Add to dashboard Cite

The Impact of Proteomics on Products and Processes

Cited by 4 publications

References 66 publications

Perfusion Culture of Hybridoma Cells for Hyperproduction of IgG_2a Monoclonal Antibody in a Wave Bioreactor‐Perfusion Culture System

Perfusion Culture of Hybridoma Cells for Hyperproduction of IgG_2a Monoclonal Antibody in a Wave Bioreactor‐Perfusion Culture System

Protein Immobilization Using Atmospheric‐Pressure Dielectric‐Barrier Discharges: A Route to a Straightforward Manufacture of Bioactive Films

Application of Genomic and Proteomic Data for Bioprocess Analysis and Optimization

Contact Info

Product

Resources

About

The Impact of Proteomics on Products and Processes

Cited by 4 publications

References 66 publications

Perfusion Culture of Hybridoma Cells for Hyperproduction of IgG2a Monoclonal Antibody in a Wave Bioreactor‐Perfusion Culture System

Perfusion Culture of Hybridoma Cells for Hyperproduction of IgG2a Monoclonal Antibody in a Wave Bioreactor‐Perfusion Culture System

Protein Immobilization Using Atmospheric‐Pressure Dielectric‐Barrier Discharges: A Route to a Straightforward Manufacture of Bioactive Films

Application of Genomic and Proteomic Data for Bioprocess Analysis and Optimization

Contact Info

Product

Resources

About

Perfusion Culture of Hybridoma Cells for Hyperproduction of IgG_2a Monoclonal Antibody in a Wave Bioreactor‐Perfusion Culture System

Perfusion Culture of Hybridoma Cells for Hyperproduction of IgG_2a Monoclonal Antibody in a Wave Bioreactor‐Perfusion Culture System