The impact of the receptor binding profiles of the vascular endothelial growth factors on their angiogenic features

Nieminen, Tiina; Toivanen, Pekka; Rintanen, Nina; Heikura, Tommi; Jauhiainen, Suvi; Airenne, Kari J.; Alitalo, Kari; Marjomäki, Varpu; Ylä‐Herttuala, Seppo

doi:10.1016/j.bbagen.2013.10.005

Cited by 25 publications

(27 citation statements)

References 75 publications

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“…VEGF is an angiogenic factor, which is involved in the regulation of the proliferation, migration and viability of endothelial cells (2,13,21,22). VEGF is found in almost all tissues in adults and shows its effects by means of the flt-1 and flk-1 receptors found on the target cells (10,11,15,22,26).…”

Section: Discussionmentioning

confidence: 99%

“…The activation of the VEGF receptors phosphorylates several intracellular signalling proteins, including among others the activator proteins phosphoinositol-3 kinase, phospholipase C and ras GTPase, and thereby, leads to the proliferation, migration and differentiation of endothelial cells (9,15,16,21,22). On the contrary to adult females, active angiogenesis does not take place in the testes of adult males (13,21). Despite this fact, reports indicate that high levels of VEGF are produced in the testes.…”

Section: Introductionmentioning

confidence: 99%

“…VEGF was first named as the vascular permeability factor (VPF), and was later described as an angiogenic factor involved in the regulation of the proliferation, migration and vitality of endothelial cells (2,4,9). VEGF, which is known to be highly distributed in embryonic tissues, has also been shown to exist in adult tissues, but its functions in adult tissues have not been fully elucidated yet (10,11,13). VEGF shows activity by means of its receptors flt-1 and flk-1.…”

Section: Introductionmentioning

confidence: 99%

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The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

BEYAZ¹

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:This study was aimed at demonstrating the immunohistochemical expression of VEGF and its receptors flt-1 and flk-1 in the testes and epididymis of the rabbit and at investigating the potential role of these molecules in testicular and epididymal physiology. The testes and epididymis of 15 male adult New Zealand rabbits constituted the material of the study. The streptavidinbiotin complex (ABC) immunohistochemical technique was used to determine the localization of VEGF and its receptors flt-1 and flk-1 in the testes and epididymis. In the testes, immunoreactivity for VEGF and its receptors flt-1 and flk-1 was observed in the Sertoli cells and spermatogonia in the seminiferous tubules throughout all of the spermatogenic stages from I to VIII and in the Leydig cells of the interstitium. Furthermore, immunoreactivity was also observed in the acrosome of the spermatids in seminiferous tubules for VEGF at stages V, VI, VII and VIII and in the acrosome of the spermatids at stages VII and VIII and the blood vessels of the interstitium for flk-1. Positive immunoreactivity, ranging from strong to weak, was detected for VEGF, flt-1 and flk-1 in the cytoplasm of the principal, apical, basal and clear cells of the epithelium lining the epididymal initial segment and the caput, corpus and cauda epididymidis. The specific localization of the VEGF protein and its receptors flt-1 and flk-1 in the testes of the rabbit suggests that these molecules could have a functional role in several processes, including spermatogonial differentiation, spermiogenesis, acrosome development in spermatids, steroidogenesis in Leydig cells, epididymal sperm maturation, and the maintenance of the bloodepididymis barrier.Keywords: Epididymis, rabbit, testis, VEGF, VEGF receptors. Tavşan testis ve epididimislerinde vasküler endoteliyal büyüme faktörü ve reseptörlerinin immunohistokimyasal lokalizasyonuÖzet: Bu çalışmanın amacı, VEGF ve reseptörleri flt-1 ile flk-1'in tavşan testis ve epididimisindeki immunohistokimyasal ekspresyonlarını belirlemek ve bu moleküllerin testis ve epididimis fizyolojisindeki potansiyel rollerini araştırmaktır. Çalışmada materyal olarak, 15 adet sağlıklı, erkek Yeni Zellanda tavşanından elde edilen testis ve epididimisler kullanıldı. VEGF ve reseptörleri flt-1 ile flk-1'in testis ve epididimisdeki lokalizasyonlarının belirlenmesi amacıyla streptavidin biotin kompleks (ABC) immunohistokimyasal yöntemi kullanıldı. Testiste, I'den VIII'e kadarki tüm spermatojenik evrelerde seminifer tubüllerdeki Sertoli hücreleri ve spermatogonialarda ve intersitisyumda Leydig hücrelerinde VEGF ve reseptörleri flt-1 ile flk-1 için immunreaktivite gözlendi. Buna ilaveten, seminifer tubüllerde V., VI., VII. ve VIII. evrelerde VEGF için, VII. ve VIII. spermatojenik evrelerde ise flk-1 için spermatidlerin akrozomlarında ve intersitisyumda kan damarlarında immunreaktivite belirlendi. Epididimiste, başlangıç segmenti, kaput, korpus ve kauda epididimidis epitelindeki prensipal, apikal ve bazal hücreleri ile clear hücrelerinin sitoplazmaların...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

BEYAZ¹

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…Unlike VEGF-A, VEGF-B 186 and VEGF-D ΔNΔC are new members of the VEGF family with different receptor-binding profiles: VEGF-B is only a VEGF receptor 1 (VEGFR-1) agonist, while VEGF-D is a VEGFR-2 and VEGFR-3 agonist 12. It has been previously shown that adenoviral gene transfer with either of these VEGFs induces angiogenesis,5 6 with VEGF-D even increasing perfusion, as measured by contrast echocardiography and microspheres 5.…”

Section: Introductionmentioning

confidence: 99%

“…It has been previously shown that adenoviral gene transfer with either of these VEGFs induces angiogenesis,5 6 with VEGF-D even increasing perfusion, as measured by contrast echocardiography and microspheres 5. Both VEGF-B 186 and VEGF-D ΔNΔC are very soluble and diffusible factors in tissues since they do not bind effectively to heparan sulfates 12. Therefore, either of these potent new VEGFs could be useful for the induction of therapeutic vascular growth in the human heart 7…”

Section: Introductionmentioning

confidence: 99%

AdVEGF-B₁₈₆and AdVEGF-D^ΔNΔCinduce angiogenesis and increase perfusion in porcine myocardium

Nurro

Halonen

Kuivanen

et al. 2016

Heart

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Effects of carbon‐ion beam irradiation on the angiogenic response in lung adenocarcinoma A549 cells

Liu

Zhang

et al. 2014

Cell Biology International

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Radiotherapy has been focused mainly on killing cancer cells, and little attention has been paid to the process supporting tumor growth and metastasis, including the process of angiogenesis. To investigate the effects of carbon-ion irradiation on angiogenesis in lung cancer cells, we examined the expression of vascular endothelial growth factor and basic fibroblast growth factor in the tumor conditioned medium (TCM) of A549 cells exposed to carbon-ion or X-ray irradiation, as well as endothelial cell growth, invasion, and tube formation induced by TCM. No changes in vascular endothelial growth factor secretion were detected in the TCM of A549 cells exposed to carbon-ion irradiation at 2 or 4 Gy, whereas 1 Gy of irradiation significantly decreased vascular endothelial growth factor and basic fibroblast growth factor levels. Carbon-ion irradiation at 1 Gy inhibited endothelial cell invasion and tube formation. The TCM from A549 cells irradiated with X-ray promoted angiogenesis, whereas the TCM of A549 cells exposed to carbon-ion irradiation at 2 or 4 Gy had no effect. These findings suggest that carbon-ion irradiation at 1 Gy significantly suppressed the process of angiogenesis in vitro by inhibiting endothelial cell invasion and tube formation, which are related to vascular endothelial growth factor and basic fibroblast growth factor production.

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The impact of the receptor binding profiles of the vascular endothelial growth factors on their angiogenic features

Cited by 25 publications

References 75 publications

The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

AdVEGF-B₁₈₆and AdVEGF-D^ΔNΔCinduce angiogenesis and increase perfusion in porcine myocardium

Effects of carbon‐ion beam irradiation on the angiogenic response in lung adenocarcinoma A549 cells

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The impact of the receptor binding profiles of the vascular endothelial growth factors on their angiogenic features

Cited by 25 publications

References 75 publications

The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

AdVEGF-B186and AdVEGF-DΔNΔCinduce angiogenesis and increase perfusion in porcine myocardium

Effects of carbon‐ion beam irradiation on the angiogenic response in lung adenocarcinoma A549 cells

Contact Info

Product

Resources

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AdVEGF-B₁₈₆and AdVEGF-D^ΔNΔCinduce angiogenesis and increase perfusion in porcine myocardium