Modern hotels are built and based on their business in such a way that they meet the modern trends on the domestic and international tourist market. The primacy is taken by the so-called "green hotels", hotels in an unpolluted, natural environment and hotels that take care of the health of the body and spirit of their guests - wellness and spa hotels. Wellness tourism is practiced by healthy people who travel to preserve and improve their health, and the concept itself implies a healthier diet, exercise, building healthy eating habits, with the main goal of achieving a balance of body, mind and spirit. Analyzing online hotel guest reviews has become the dominant method for evaluating hotel performance and evaluating guest experience based on services and tangible products provided. For the purposes of this paper, an analysis of data from one of the leading online accommodation reservation platforms, Booking.com, was performed. Currently (data from March 2024), there are 1,206 accommodation facilities in Serbia that offer spa centers located in its most attractive tourist regions. There are 483 wellness and spa hotels, categorized with 5, 4 and 3 stars, and the average rating of service users for the top 10 hotels ranges from 9.1 (excellent) to 9.5 (exceptional). The main goal in this paper is to segment and define profiles of tourists based on their ratings (reviews) of the offer and services provided in wellness and spa hotels in Serbia. Segmentation can help hotels identify the preferences and needs of tourists, as well as design and implement different strategies for those target segments, in order to increase customer satisfaction, build loyalty and ensure profitability. As a very good source of data, guest reviews brought new insights into the business process and indirectly influenced the improvement of marketing activities.