The importance of the bacterial cell wall in uranium(<scp>vi</scp>) biosorption

Hufton, Joseph; Harding, John H.; Smith, Thomas J.; Romero-González, María

doi:10.1039/d0cp04067c

Cited by 38 publications

(6 citation statements)

References 72 publications

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“…Membranes play a major role in sorption of metals where electrostatic interactions between positively charged metal species and negatively charged functional groups (e.g., phosphates and carboxyles) occur. Gram-positive bacteria (such as the Be9 strain) exhibit greater biosorption capacity than Gram-negative bacteria due to their cell envelope components ( Hufton et al, 2021 ). Liu et al (2015) demonstrated the role of carboxyl, phosphoryl, and amino functional groups of Synechococcus sp.…”

Section: Discussionmentioning

confidence: 99%

Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Martínez-Rodríguez

Sánchez-Castro²,

Ojeda³

et al. 2023

Front. Microbiol.

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IntroductionIndustrial activities related with the uranium industry are known to generate hazardous waste which must be managed adequately. Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomineralization-based methods, mediated by microbial enzymes (e.g., phosphatase), have been proposed as a promising approach. However, the presence of different forms of phosphates in these environments plays a complicated role which must be thoroughly unraveled to optimize results when applying this remediation process.MethodsIn this study, we have looked at the effect of different phosphate sources on the uranium (U) biomineralization process mediated by Microbacterium sp. Be9, a bacterial strain previously isolated from U mill tailings. We applied a multidisciplinary approach (cell surface characterization, phosphatase activity, inorganic phosphate release, cell viability, microscopy, etc.).Results and DiscussionIt was clear that the U removal ability and related U interaction mechanisms by the strain depend on the type of phosphate substrate. In the absence of exogenous phosphate substrate, the cells interact with U through U phosphate biomineralization with a 98% removal of U within the first 48 h. However, the U solubilization process was the main U interaction mechanism of the cells in the presence of inorganic phosphate, demonstrating the phosphate solubilizing potential of the strain. These findings show the biotechnological use of this strain in the bioremediation of U as a function of phosphate substrate: U biomineralization (in a phosphate free system) and indirectly through the solubilization of orthophosphate from phosphate (P) containing waste products needed for U precipitation.

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Section: Discussionmentioning

confidence: 99%

Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Martínez-Rodríguez

Sánchez-Castro²,

Ojeda³

et al. 2023

Front. Microbiol.

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“…The harvested cells from one suspension were subjected to vacuum drying overnight and the dry weight was measured. The other suspension was subjected to sonication to break cells (complete cell disruption was confirmed by microscopy), and the insoluble fraction containing cell wall and wall-associated proteins ( 55 ) was washed by Milli-Q water. We confirmed that protein mass in the insoluble fraction per total cell mass was about 0.2% based on the Bradford Assay (Bio-Rad Laboratories, Inc.), and, thus the insoluble fraction predominantly consists of cell wall.…”

Section: Methodsmentioning

confidence: 99%

The role of cell-envelope synthesis for envelope growth and cytoplasmic density in Bacillus subtilis

et al. 2022

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All cells must increase their volumes in response to biomass growth to maintain intracellular mass density within physiologically permissive bounds. Here, we investigate the regulation of volume growth in the Gram-positive bacterium Bacillus subtilis. To increase volume, bacteria enzymatically expand their cell envelopes and insert new envelope material. First, we demonstrate that cell-volume growth is determined indirectly, by expanding their envelopes in proportion to mass growth, similarly to the Gram-negative Escherichia coli, despite their fundamentally different envelope structures. Next, we studied, which pathways might be responsible for robust surface-to-mass coupling: We found that both peptidoglycan synthesis and membrane synthesis are required for proper surface-to-mass coupling. However, surprisingly, neither pathway is solely rate-limiting, contrary to wide-spread belief, since envelope growth continues at a reduced rate upon complete inhibition of either process. To arrest cell-envelope growth completely, the simultaneous inhibition of both envelope-synthesis processes is required. Thus, we suggest that multiple envelope-synthesis pathways collectively confer an important aspect of volume regulation, the coordination between surface growth and biomass growth.

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“…Rhizobium immobilizes metal ions on the cell surface through mechanisms such as complexation, coordination, and ion exchange, thereby preventing plant root cells from absorbing excess metals from the soil and causing toxicity [ 120 , 121 ]. For example, phosphate and carboxyl groups associated with Rhizobium cell envelope proteins and phosphorylated biopolymers (such as teichoic acid, surface proteins, and peptidoglycan) mediate uranium (Ur) biosorption, leading to greater accumulation of Ur in the cell envelope [ 122 ]. The mycelium on the surface of the AMF structure adsorbs metal ions and deposits them on the cell wall, and then reduces TMs from one form to another by positively charged particles, including amino acids, thiol groups, and glutathione, etc., to reduce toxicity to plants [ 123 , 124 ].…”

Section: Detoxification Regulation Strategies Of Rhizobium Symbiosis ...mentioning

confidence: 99%

Signaling and Detoxification Strategies in Plant-Microbes Symbiosis under Heavy Metal Stress: A Mechanistic Understanding

Liu

et al. 2022

Microorganisms

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Plants typically interact with a variety of microorganisms, including bacteria, mycorrhizal fungi, and other organisms, in their above- and below-ground parts. In the biosphere, the interactions of plants with diverse microbes enable them to acquire a wide range of symbiotic advantages, resulting in enhanced plant growth and development and stress tolerance to toxic metals (TMs). Recent studies have shown that certain microorganisms can reduce the accumulation of TMs in plants through various mechanisms and can reduce the bioavailability of TMs in soil. However, relevant progress is lacking in summarization. This review mechanistically summarizes the common mediating pathways, detoxification strategies, and homeostatic mechanisms based on the research progress of the joint prevention and control of TMs by arbuscular mycorrhizal fungi (AMF)-plant and Rhizobium-plant interactions. Given the importance of tripartite mutualism in the plant-microbe system, it is necessary to further explore key signaling molecules to understand the role of plant-microbe mutualism in improving plant tolerance under heavy metal stress in the contaminated soil environments. It is hoped that our findings will be useful in studying plant stress tolerance under a broad range of environmental conditions and will help in developing new technologies for ensuring crop health and performance in future.

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The importance of the bacterial cell wall in uranium(vi) biosorption

Abstract: The bacterial cell envelope, in particular the cell wall, is considered the main controlling factor in the biosorption of aqueous uranium(vi) by microorganisms.

Cited by 38 publications

References 72 publications

Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

The role of cell-envelope synthesis for envelope growth and cytoplasmic density in Bacillus subtilis

Signaling and Detoxification Strategies in Plant-Microbes Symbiosis under Heavy Metal Stress: A Mechanistic Understanding

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