The In Vitro Sensitivity to Brucellergen of Leucocytes from Guinea Pigs Experimentally Infected with Brucella Abortus

Demonstration of Delayed‐type Hypersensitivity in Guinea‐pigs by the Agarose Plate Technique

“…The present study confirms the results of (5) with tuberculin and brucellergen in cultures of peripheral blood leucocytes from Brucella-infected guinea-pigs. Later, using the capillary tube technique, David et al ( 6 ) showed that the inhibition of migration of PEC from guinea-pigs immunized with diphtheria toxoid and ovalbumin is specific.…”

Cellular hypersensitivity to components of intestinal mucosa in ulcerative colitis and Crohn's disease

“…Animal experiments with immunocompetent cells from peritoneal exudate, lymph nodes, spleen, bone marrow, and peripheral blood indicate that the migration inhibition response is a parameter of cellular hypersensitivity (Rich and Lewis, 1932;Moen and Swift, 1936;Raffel, 1948;Heilman, Howard, and Carpenter, 1958;Darlington and Scherago, 1960;Johnson and Scherago, 1960;Svejcar and Johanovsk', 1961;George and Vaughan, 1962;Heilman, 1963;Carpenter and Brandriss, 1964;David, 1964a and1964b) and a cell population from human peripheral blood has been shown to possess the same kind of reactivity (S0borg and Bendixen, 1967;S0borg 1967 and1968).…”

Leukocytic Hypersensitivity in Experimental Group A Streptococcal Infections

“…Leukocyte migration studies with type 1 antigen were performed 10 days after the last of the five injections. The cytotoxic indices of the leukocytes dropped markedly as a result of the injections of a different streptococcal type (type 3 as the result of the injections of the same type killed by heat (Group III), nor of the reinjection of the original type (Group I).…”

“…Scherago and his co-workers (3,4,8,15) suggested that the leukocytic hypersensitivity is associated only with active infection. It may be that during the active stage of infection a larger proportion of cells becomes sensitized, some of which become distributed or are held in the blood.…”

“…Gerlach, Scherago, and Hall (4) reported that the hypersensitivity of the leukocytes from tuberculous patients to tuberculin also decreased after chemotherapy although the skin sensitivity did not. Darlington and Scherago (3) found that, in guinea pigs that were experimentally infected with Brucella abortus, skin sensitivity developed the 1st week after infection, whereas leukocytic sensitivity did not appear until the 4th week, at which time the animals had developed bilateral orchitis. The leukocytic sensitivity persisted until the 8th week, about as long as the orchitis did, but the skin was still sensitive at the 10th week.…”