The Incorporation and Degradation of Pyrimidine DNA Precursors by Human Leucocytes

Cooper, Ed; Milton, J.D.

doi:10.1038/bjc.1964.81

Cited by 40 publications

(4 citation statements)

References 9 publications

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“…The parallel course of the two curves in Figure la further shows that there is no isotope effect. Although this is to be expected, an experimental confirmation is necessary (Cooper & Milton, 1964). From the curves in Figure l a another conclusion can be drawn.…”

Section: S Phase Duration Of Mouse Jejunal Crypt Cells Determined By mentioning

confidence: 87%

Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studies

1990

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Abstract. The present experiments with [14C]‐thymidine (TdR) and [3H]‐bromo‐deoxyuridine (BrdU) using mouse jejunal crypt cells show that the upper limit of the tracer dose of TdR is about 0.5 µg g body weight‐1 and that of BrdU is about 5·0 µg g body weight‐1. Applying these doses, the proportions of the endogenous DNA synthesis attributed to the exogenous DNA precursor are 2% and 9% respectively. For [3H]‐TdR doses commonly used in cell kinetic studies this proportion is only 0‐1‐1.0%, a negligible quantity that does not influence the endogenous DNA synthesis. The maximum availability time of tracer doses of TdR as well as BrdU is 40 to 60 min, the majority of the precursors being incorporated after 20 min. The availability time is the same for TdR doses exceeding the tracer dose by a factor of 80, whereas it is prolonged in the case of BrdU doses exceeding the tracer dose by a factor of 50. BrdU is suitable to replace radioactively labelled TdR in short term cell kinetic studies, i.e. determination of the labelling index or of the S phase duration by double labelling. However, more studies are needed to elucidate how far BrdU can replace TdR in long term studies as shown by differences between the fraction of labelled mitoses (FLM) curves of a human renal cell carcinoma measured with BrdU and [3H]‐TdR.

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Section: S Phase Duration Of Mouse Jejunal Crypt Cells Determined By mentioning

confidence: 87%

Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studies

1990

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“…There is no agreement of the influence of these latter cells on lymphocyte reactivity in vitro. Furthermore, they die rapidly in vitro and release enzymes and other substances which may influence thymidine incorporation and probably act on other cells [4,7,13,14]. It seems therefore desirable to work with granulocyte-poor cell suspen sions for culture.…”

Section: Discussionmentioning

confidence: 99%

Effect of Polyspecific Rabbit Anti–Immunoglobulin Antisera on Human Lymphocytes <i>in vitro</i>

Natvig²

1970

Int Arch Allergy Immunol

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“…dish containing 50 unsterilized onion seeds just before germination was added to -H-thymidine (0.05 ml containing 50 ,uc: 0.008 ,umole) and aliquots taken at intervals. Left to right: 22 hr, 6 hr, 2 PLANT PHYSIOLOGY water from a dish in which gas-treated peas remained for 24 to 48 hr after seed treatment was added to a radiothymidine solution, there was no conversion of thymidine to thymine or intermediate products ( fig 1A).…”

Section: Methodsmentioning

confidence: 99%

Effectiveness of Certain Sterilizing Agents for Seedling Incubation in Radiothymidine

McQuade¹,

Atchison²,

Parisi³

1969

Plant Physiol.

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Abstract. Failure of seed sterilization methods and breakdown in sterile technique can result in catabolism of thymidine by microorganisms during seedling incubation in radioisotope. The effectiveness of some sterilizing agents was monitored by microbiological methods and by thin layer chromatography for the presence of thymidine degradation products. Sodium hypochlorite and ethylene oxide each sterilized pea and sunflower seeds. Sodium hypochlorite failed to sterilize onion seeds while ethylene oxide was only occasionally effective and often retarded germination. Captan, commonly used before germination to prevent fungal blight of seedlings, did not reduce the bacterial flora of onion seeds.

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The Incorporation and Degradation of Pyrimidine DNA Precursors by Human Leucocytes

Cited by 40 publications

References 9 publications

Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studies

Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studies

Effect of Polyspecific Rabbit Anti–Immunoglobulin Antisera on Human Lymphocytes <i>in vitro</i>

Effectiveness of Certain Sterilizing Agents for Seedling Incubation in Radiothymidine

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