The increase in bilayer fluidity of rat liver plasma membranes achieved by the local anesthetic benzyl alcohol affects the activity of intrinsic membrane enzymes.

Gordon, Larry M.; Sauerheber, Richard; Esgate, Judy A.; Dipple, I; Marchmont, Robert J.; Houslay, Miles D.

doi:10.1016/s0021-9258(19)85523-0

Cited by 261 publications

(18 citation statements)

References 52 publications

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“…The effect of dietary DHA on the fluidity of the BCM, in the present investigation, and of synaptosomes, in studies by others (16), contrasts with the unaltered hepatocyte plasma membrane fluidity in rats fed PUFA through black currant oil diets (27). Nevertheless, the effect of dietary DHA on 5Ј-nucleotidase activity in the BCM is consistent with its increased activity after liver plasma membrane fluidity is enhanced (45). Likewise, a significant decrease in stearic acid, which is reported to have membrane-rigidifying properties, in the BCM of DHA-fed rats ( 15) is in accordance with the fact that the BCM of the DHA-fed rats is more fluid than that of the control rats.…”

Section: Discussionsupporting

confidence: 72%

Effects of docosahexaenoic acid on annular lipid fluidity of the rat bile canalicular plasma membrane

Hashimoto

Hossain

Shimada

et al. 2001

Journal of Lipid Research

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The role of docosahexaenoic acid (DHA) in the fluidity of the annular lipid regions and their associated membrane-bound proteins is still not as well understood as that in the global (bulk) lipid regions. We therefore studied the effects of dietary DHA on the relationship between annular and global lipid fluidity and membrane-bound enzymes such as 5 -nucleotidase and Mg 2 ؉ -ATPase in the rat bile canalicular membrane. Dietary DHA caused significant increases in 5nucleotidase and Mg 2 ؉ -ATPase activity and in global and annular lipid fluidity, a higher increase in fluidity in the annular lipids than the global lipids, and a decrease in the cholesterol-tophospholipid molar ratio in the canalicular membrane. Plasma total cholesterol and LDL cholesterol decreased, and fecal cholesterol increased in the DHA-fed rats. No changes were observed in oxidative markers, but glutathione peroxidase increased in the liver with DHA feeding. Annular lipid fluidity, but not global lipid fluidity, correlated remarkably well with DHA, synchronously with the activities of 5 -nucleotidase and Mg 2 ؉ -ATPase. The data indicate that the DHA-induced increase in annular lipid fluidity is responsible for the increases observed in the enzyme activity. We therefore concluded that the increased activity of membrane-bound enzymes and transporters induced by DHA and the concomitant increase in annular lipid fluidity comprise one of the mechanisms involved in DHA-induced clearance of plasma cholesterol. -

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Section: Discussionsupporting

confidence: 72%

Effects of docosahexaenoic acid on annular lipid fluidity of the rat bile canalicular plasma membrane

Hashimoto

Hossain

Shimada

et al. 2001

Journal of Lipid Research

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“…Hence, we might expect an aggravation of the clustering phenotype were we to further destabilize the NE membranes. To test this hy pothesis, we used benzyl alcohol (BA), a reagent that fluidizes and destabilizes membranes (Gordon et al, 1980) and which has been extensively used to investigate membrane dynamics and even membrane-NPC interactions in yeast and other fungi (Scarcelli et al, 2007;Liu et al, 2009;Titus et al, 2010). Although BA does not affect the stability of the purified Nup84 complex (Fig.…”

Section: Nup84 Complexmentioning

confidence: 99%

Structure–function mapping of a heptameric module in the nuclear pore complex

Fernández-Martı́nez

Phillips

Sekedat

et al. 2012

Journal of Cell Biology

116

187

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“…This suggests that the apq12∆-associated phenotypes might be caused by the mutant strain's inability to modulate membrane composition at lower temperatures. To test this hypothesis, we added low levels of BA to the media to increase membrane fl uidity (Colley and Metcalfe, 1972;Gordon et al, 1980). apq12∆ cells expressing Nup82-GFP were incubated overnight at 37°C and shifted to 23°C for 10 h with or without 0.4% BA.…”

Section: Alteration Of Membrane Dynamics Suppresses Nup Mislocalization In Apq12𝚫 Cellsmentioning

confidence: 99%

“…In addition, cells lacking Apq12 displayed defects both in NPC biogenesis and in the morphology of the NE. We suggest that these defects are caused by alterations in the dynamics and properties of the NE because the proper localization of Nups in apq12∆ cells was restored upon addition to the medium of benzyl alcohol (BA), which is thought to increase the fl uidity and fl exibility of membranes (Colley and Metcalfe, 1972;Gordon et al, 1980). Thus, it is likely that the reported defects in mRNA export, pre-mRNA processing, and cell division of apq12∆ cells are indirect consequences of altered membrane dynamics.…”

Section: Introductionmentioning

confidence: 98%

The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes

Scarcelli

Hodge

Cole

2007

The Journal of Cell Biology

148

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Although the structure and function of components of the nuclear pore complex (NPC) have been the focus of many studies, relatively little is known about NPC biogenesis. In this study, we report that Apq12 is required for efficient NPC biogenesis in Saccharomyces cerevisiae. Apq12 is an integral membrane protein of the nuclear envelope (NE) and endoplasmic reticulum. Cells lacking Apq12 are cold sensitive for growth, and a subset of their nucleoporins (Nups), those that are primarily components of the cytoplasmic fibrils of the NPC, mislocalize to the cytoplasm. APQ12 deletion also causes defects in NE morphology. In the absence of Apq12, most NPCs appear to be associated with the inner but not the outer nuclear membrane. Low levels of benzyl alcohol, which increases membrane fluidity, prevented Nup mislocalization and restored the proper localization of Nups that had accumulated in cytoplasmic foci upon a shift to lower temperature. Thus, Apq12p connects nuclear pore biogenesis to the dynamics of the NE.

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The increase in bilayer fluidity of rat liver plasma membranes achieved by the local anesthetic benzyl alcohol affects the activity of intrinsic membrane enzymes.

Cited by 261 publications

References 52 publications

Effects of docosahexaenoic acid on annular lipid fluidity of the rat bile canalicular plasma membrane

Effects of docosahexaenoic acid on annular lipid fluidity of the rat bile canalicular plasma membrane

Structure–function mapping of a heptameric module in the nuclear pore complex

The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes

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