The individual-specific and diverse nature of the preterm infant microbiota

Barrett, Eva; Kerr, Colm; Murphy, Kiera; O'Sullivan, Órla; Ryan, C. Anthony; Dempsey, Eugene M.; Murphy, Brendan P.; O’Toole, Paul W.; Cotter, Paul D.; Fitzgerald, Gerald F.; Ross, R. Paul; Stanton, Catherine

doi:10.1136/archdischild-2012-303035

Cited by 107 publications

(91 citation statements)

References 36 publications

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“…Increased levels of Proteobacteria have been reported extensively by studies assessing the composition of gut microbiota of preterm infants 14,[20][21][22] and interestingly it has lately been linked to necrotizing enterocolitis. 14,22,23 The dominance of Staphylococcaceae in the gut microbiota of newborns has been hypothesized to be the result of improved hygienic condition during delivery.…”

Section: Discussionmentioning

confidence: 99%

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The development of gut microbiota in critically ill extremely low birth weight infants assessed with 16S rRNA gene based sequencing

et al. 2014

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Objective: an increasing number of studies that are using high-throughput molecular methods are rapidly extending our knowledge of gut microbial colonization in preterm infants whose immaturity and requirement for extensive treatment may result in altered colonization process. We aimed to describe the profile of gut microbiota in 50 extremely low birth weight (<1200 g) critically ill infants at three different time points during the first two months of life by using 16S rrNa gene specific sequencing.Patients and Methods: Stool samples were collected at the age of one week, one month and two months. Bacterial community profiling was done using universal amplification of 16S rrNa gene and 454 pyrosequencing.results: The diversity of gut microbiota in preterm neonates in the first week of life was low but increased significantly over two months. The gut microbiota was dominated by facultative anaerobic bacteria (Staphylococcus spp. and Enterobacteriaceae) and lacked colonization with bacteria known to provide resistance against pathogens (Bacteroides, Bifidobacterium, and Lactobacillus) throughout the study. Colonization of Escherichia coli and uncultured Veillionella was positively correlated with maturity. Infants born to mothers with chorioamnionitis had significantly higher bacterial diversity than those without.Conclusions: High prevalence and abundance of potentially pathogenic Enterobacteriaceae and Staphylococcaceae with low prevalence and abundance of colonization resistance providing taxa bifidobacteria, Bacteroides and lactobacilli may lead to high infection risk via microbial translocation from the gut. additionally, our data suggest that maternal chorioamnionitis may have an effect on the diversity of infants' gut microbiota; however, the mechanisms involved remain to be elucidated.

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Section: Discussionmentioning

confidence: 99%

“…Starting day of total enteral feeding; median (IQr) 17 (14)(15)(16)(17)(18)(19)(20)(21)(22) Number of participants suffering from sepsis: 24 early onset sepsis 2…”

Section: Formula 21mentioning

confidence: 99%

The development of gut microbiota in critically ill extremely low birth weight infants assessed with 16S rRNA gene based sequencing

et al. 2014

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“…These results suggest that stress during this vulnerable period of microbiota gut brain axis development leads to dysfunction within several of the intertwined components and ultimately may increase vulnerability to disease. Pre-term infants have a considerably different GI microbiota profile than their full-term counterparts (Fanaro et al, 2003, Barrett et al, 2013 which correlates with delayed brain development.…”

Section: Postnatal Implications Of the Microbiome On Brain Developmentmentioning

confidence: 99%

Early-life adversity and brain development: Is the microbiome a missing piece of the puzzle?

et al. 2017

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“…Total genomic DNA was extracted from respective filters as described previously in Barrett et al (2013). MST qPCR assay conditions were identical to those described by Kildare et al (2007).…”

Section: Molecular Analysismentioning

confidence: 99%

“…These assays target regions of the Bacteroidales bacteria that are specific to human (BacHum) and cow (BacCow) fecal material. Total genomic DNA extraction followed the protocol of Barrett et al (2013) with assay conditions for quantitative PCR (qPCR) carried out following the protocol of Kildare et al (2007) …”

Section: Molecular Analysismentioning

confidence: 99%

Using microbiological tracers to assess the impact of winter land use restrictions on the quality of stream headwaters in a small catchment

Flynn

Deakin

Archbold

et al. 2016

Science of The Total Environment

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The individual-specific and diverse nature of the preterm infant microbiota

Cited by 107 publications

References 36 publications

The development of gut microbiota in critically ill extremely low birth weight infants assessed with 16S rRNA gene based sequencing

The development of gut microbiota in critically ill extremely low birth weight infants assessed with 16S rRNA gene based sequencing

Early-life adversity and brain development: Is the microbiome a missing piece of the puzzle?

Using microbiological tracers to assess the impact of winter land use restrictions on the quality of stream headwaters in a small catchment

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