The influence of fillers and protease inhibitors in experimental resins in the protein profile of the acquired pellicle formed in situ on enamel-resin specimens

Pelá, Vinícius Taioqui; Prakki, Anuradha; Wang, Linda; Ventura, Talita Mendes Oliveira; Silva, Cíntia Maria de Souza e; Cassiano, Luiza Paula Silva; Brianezzi, L. F. F.; Leite, Aline L.; Buzalaf, Marília Afonso Rabelo

doi:10.1016/j.archoralbio.2019.104527

Cited by 9 publications

(4 citation statements)

References 36 publications

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“…During this period, the volunteers were not allowed to eat or drink. The device was then removed from the mouth; the specimens were washed with deionized water and dried (17). For AEP harvesting, the same procedure was performed as described for the in vitro condition.…”

Section: Methodsmentioning

confidence: 99%

“…This, on the one hand, allows for the recovery of larger amounts of proteins to be analyzed (11‐13), but on the other hand, the composition of the AEP changes according to its location in the dental arches (10). In this regard, some in situ studies have also been performed (14‐17), in which the AEP has been collected only from some tooth specimens that can be precisely placed at defined locations within the volunteer’s mouth, thus lowering the variations arising from location in the dental arches. Though in situ conditions can decrease AEP variations, these studies are more difficult to conduct because they require specialized preparation of dental specimens as well as a team of volunteers who are willing to wear oral appliances for longer periods.…”

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confidence: 99%

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Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Pelá

Lunardelli

Ventura

et al. 2020

European J Oral Sciences

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This study compared the protein profile of the acquired enamel pellicle (AEP) formed under three conditions: in vitro, in situ, and in vivo. Nine volunteers participated in all procedures. In the in vitro condition, the volunteers donated saliva, in which specimens were incubated to form the AEP. In the in situ condition, the volunteers used an oral device containing specimens where the AEP was formed. In the in vivo condition, the AEP was collected from the volunteers own teeth. All AEPs were formed for 120 min, collected and processed by mass spectrometry. Overall, a total of 321 proteins were identified, among which 37 proteins are commonly considered typical in the AEP. For each of the in vitro, in situ, and in vivo conditions, respectively, 66, 174, and 170 proteins were identified. For the in vitro condition, 17 pellicle-typical proteins were not identified. Furthermore, several proteins with important functions within the AEP presented differences in expression in the three conditions. The qualitative profile of the proteins, especially the typical ones, is different in the in vitro condition. In addition, there are important quantitative differences that may interfere when attempting to extrapolate in vitro results to an in situ and in vivo condition.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Pelá

Lunardelli

Ventura

et al. 2020

European J Oral Sciences

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“…Meanwhile, it was observed that in the case of resin-based composites, the kind of filler particles also seem to significantly influence the adsoption of proteins to the material surface. It was shown that the incorporation of fillers generally increased the abundance of salivary pellicle proteins, and that the addition of inhibitors generally increased cystatins, lysozymes, and mucins regardless of the specific inhibitor used [20,51]. Other authors used SDS-PAGE to investigate salivary pellicle composition and concluded that human salivary low-molecular-weight mucin proline-rich proteins and agglutinin were enriched on a glass ionomer cement compared to a resin composite (TPH Spectrum, Dentsply De-Trey) [20,52].…”

Section: Discussionmentioning

confidence: 99%

Salivary Pellicle Formed on Dental Composites Evaluated by Mass Spectrometry—An In Situ Study

Reise,

Kranz,

Heyder

et al. 2023

Molecules

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(1) Background: In the oral environment, sound enamel and dental restorative materials are immediately covered by a pellicle layer, which enables bacteria to attach. For the development of new materials with repellent surface functions, information on the formation and maturation of salivary pellicles is crucial. Therefore, the present in situ study aimed to investigate the proteomic profile of salivary pellicles formed on different dental composites. (2) Methods: Light-cured composite and bovine enamel samples (controls) were exposed to the oral cavity for 30, 90, and 120 min. All samples were subjected to optical and mechanical profilometry, as well as SEM surface evaluation. Acquired pellicles and unstimulated whole saliva samples were analyzed by SELDI–TOF–MS. The significance was determined by the generalized estimation equation and the post-hoc bonferroni adjustment. (3) Results: SEM revealed the formation of homogeneous pellicles on all test and control surfaces. Profilometry showed that composite surfaces tend to be of higher roughness compared to enamel. SELDI–TOF–MS detected up to 102 different proteins in the saliva samples and up to 46 proteins in the pellicle. Significant differences among 14 pellicle proteins were found between the composite materials and the controls. (4) Conclusions: Pellicle formation was material- and time-dependent. Proteins differed among the composites and to the control.

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“…An essential step in the development of secondary caries is the formation of acquired pellicle around enamel‐restoration interface, which releases acidic by‐products that destroy the dentin margin, causing further diffusion (Zarella et al, 2016). A recent experiment has shown that EGCG can positively influence the protein profile of acquired pellicle (AP), thus protecting periodontal tissue, reducing bacterial colonization, and having anti‐caries effect (Pela et al, 2019).…”

Section: Materials and Egcgmentioning

confidence: 99%

Application of green tea extracts epigallocatechin‐3‐gallate in dental materials: Recent progress and perspectives

Liao

Tang

Chu

et al. 2020

J Biomedical Materials Res

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Because of excellent biocompatibility, antioxidant activity, and anti-caries ability, epigallocatechin-3-gallate (EGCG) has been widely studied in the treatment of oral diseases, such as periodontal disease, oral cancer, and dental caries. To reach the site of the lesion or achieve sustained release, play the role of anti-caries, anti-inflammatory, or to maintain or improve the physical properties of the modified material， EGCG need to be cross-linked or embedded with dental adhesives, barrier membranes, bone replacement materials, tissue regeneration materials, and antimicrobial anti-caries materials to better prevent or treat oral diseases. This article reviews the applications of EGCG in oral materials, involving various areas of the oral cavity, reveals their excellent potential, and sees shortcomings in these research to promote the better development of EGCG applications in oral materials such as oral repair materials, bone tissue engineering materials and antibacterial and anti-caries materials.

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The influence of fillers and protease inhibitors in experimental resins in the protein profile of the acquired pellicle formed in situ on enamel-resin specimens

Cited by 9 publications

References 36 publications

Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Salivary Pellicle Formed on Dental Composites Evaluated by Mass Spectrometry—An In Situ Study

Application of green tea extracts epigallocatechin‐3‐gallate in dental materials: Recent progress and perspectives

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