The Influence of <i>Phytophthora citricola</i> on Rhizosphere Soil Solution Chemistry and the Nutritional Status of European Beech Seedlings

Wang, Z. Y.; Göttlein, Axel; Rodenkirchen, H.; Fleischmann, F.; Oßwald, Wolfgang

doi:10.1046/j.1439-0434.2003.00729.x

Cited by 9 publications

(14 citation statements)

References 16 publications

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“…These investigations showed that P. citricola transiently reduced carbon assimilation of beech. Furthermore, P. citricola infection diminished the uptake of mineral nutrients as well as the nutrient status of leaves (Fleischmann et al 2004;Wang et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Effects of ozone and Phytophthora citricola on non-structural carbohydrates of European beech (Fagus sylvatica) saplings

2009

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A lysimeter study was performed to monitor long term effects of chronic ozone enrichment on saplings of European beech (Fagus sylvatica L). After 3 years of ozone exposure a root infection with Phytophthora citricola Swada was established in the fourth year to study the interaction between elevated ozone and the root infection on the carbon budget of beech saplings. By using quantitative PCR no differences in root infection with P. citricola were observed between the ozone treatments. In contrast to the first 3 years of ozone exposure, sucrose and starch concentrations in leaves were diminished in ozone treated plants in the fourth year. The root infection reduced sucrose concentrations in leaves. Starch reserves of the heterotrophic biomass were not affected by any treatments. Thus 4 years of ozone exposure and 1 year of P. citricola root infection had only limited effect on carbohydrate metabolism in beech saplings.

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Section: Introductionmentioning

confidence: 99%

Effects of ozone and Phytophthora citricola on non-structural carbohydrates of European beech (Fagus sylvatica) saplings

2009

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“…P. citricola is a commonly isolated pathogen in central Europe with a broad range of economically important hosts such as Q. robur and Q. petraea (Jung et al ., 2000; Thomas et al ., 2002) and F. sylvatica (Jung et al ., 2005; Nechwatal and Oβwald, 2001; Wang et al ., 2003; Werres, 1995). P. citricola was the most frequently isolated Phytophthora species on beech trees in Europe (Jung et al ., 2005; Nechwatal and Oβwald, 2001) and during extensive surveys to monitor P. ramorum in England and Wales was also the most frequently isolated Phytophthora species from rhododendron leaves (Hughes et al ., 2006).…”

Section: Introductionmentioning

confidence: 99%

Detection and quantification of Phytophthora ramorum, P. kernoviae, P. citricola and P. quercina in symptomatic leaves by multiplex real‐time PCR

Schena¹,

Hughes²,

Cooke³

2006

Molecular Plant Pathology

146

131

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SUMMARY New species of Phytophthora such as Phytophthora ramorum, P. kernoviae and P. quercina together with P. citricola are plant pathogens which impact on forest health, natural ecosystem stability and international trade. A real-time multiplex PCR approach based on TaqMan PCR was developed to simultaneously identify and detect these four Phytophthora species. Specific primers and probes labelled with FAM (P. ramorum), Yakima Yellow (P. kernoviae), Rox (P. citricola) and Cy5 (P. quercina) were designed in different regions of the ras-related protein (Ypt1) gene. A new set of Black Hole Quenchers (BHQ), which dissipate energy as heat rather than fluorescence, were utilized. The method proved to be highly specific in tests with target DNA from 72 Phytophthora isolates (35 species). For all pathogens, the detection limit was 100 fg of target DNA and was not improved utilizing a nested approach to provide a first round of amplification with Phytophthora spp.-specific primers. Cycle threshold (Ct) values were linearly correlated with the concentration of the target DNA (correlation coefficients ranged from 0.947 to 0.996) and were not affected by the presence of plant extracts, indicating the appropriateness of the method for qualitative and quantitative analyses. Two universal primers and a TaqMan probe were also developed to evaluate the quality and quantity of extracted DNA and to avoid false negatives. The reliability of the entire procedure was assessed using both artificially and naturally infected leaves of a range of plant species. The method, combined with a rapid procedure for DNA extraction, proved to be rapid, reliable, sensitive and cost effective as multiple pathogens were detected within the same plant extract by using different primer/probe combinations.

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“…Hence in this study a decreased SRL may rather indicate a decreased nutrient uptake by fine roots than decreased nutrient availability. Wang et al (2003) showed that P. citricola infestation reduced nutrient uptake by fine roots of beech seedlings. The decrease in root morphological parameters due to P. citricola infection in the 20-40 cm deep slice was contrasted by an increase in root biomass that was also observed between 60 and 100 cm depth.…”

Section: Root Morphologymentioning

confidence: 99%

Do chronic aboveground O3 exposure and belowground pathogen stress affect growth and belowground biomass partitioning of juvenile beech trees (Fagus sylvatica L.)?

et al. 2009

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The impact of chronic free air ozone (O 3 ) exposure and belowground pathogen stress on growth and total biomass development of young beech trees (Fagus sylvatica L.) was investigated in a lysimeter study. Plants were growing during four years under ambient or elevated atmospheric O 3 concentrations. Additionally, in the last vegetation period the root rot pathogen Phytophthora citricola was introduced to study the interaction of ozone exposure and pathogen stress in the soil-plant system. A complete harvest at the end of the experiment enabled for the first time the assessment of fine and coarse root biomass of individual trees with a high vertical resolution down to two meter depth. Plant growth was significantly reduced by elevated ozone but not affected by P. citricola. Biomass partitioning between fine and coarse roots as well as vertical root distribution were significantly affected by both factors, whereas changes in root/shoot biomass ratio were not observed.

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The Influence of Phytophthora citricola on Rhizosphere Soil Solution Chemistry and the Nutritional Status of European Beech Seedlings

Cited by 9 publications

References 16 publications

Effects of ozone and Phytophthora citricola on non-structural carbohydrates of European beech (Fagus sylvatica) saplings

Effects of ozone and Phytophthora citricola on non-structural carbohydrates of European beech (Fagus sylvatica) saplings

Detection and quantification of Phytophthora ramorum, P. kernoviae, P. citricola and P. quercina in symptomatic leaves by multiplex real‐time PCR

Do chronic aboveground O3 exposure and belowground pathogen stress affect growth and belowground biomass partitioning of juvenile beech trees (Fagus sylvatica L.)?

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