The influence of optimal proportions of antigen and antibody in the serum precipitation reaction

Dean, H. R.; Webb, Rodney A.

doi:10.1002/path.1700290412

Cited by 87 publications

(39 citation statements)

References 4 publications

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“…Titrations were performed by the method of Dean and Webb (1926) as described by Taylor, Adair and Adair (1932). The antigen-antibody ratios assigned to the experiments were the whole numbers nearest the mean of the ratios of the first two tubes to particulate; when the rates of particulation in the tubes on either side of the optimal one were the same, the ratio of the optimal tube was recorded, as in the horse serum series in Table I.…”

Section: Methodsmentioning

confidence: 99%

“…IN titrations of anti-horse sera against parallel dilutions of (a) normal horse serum, (b) horse serum globulin and (c) horse serum albumin, Taylor, Adair and Adair (1932) found that with some antisera a primary or main zone of optimal particulation (Dean and Webb, 1926) occurred in the (a) horse serum and (b) globulin series but was absent from the (c) albumin series: the precipitin was regarded as antiglobulin. With other anti-horse sera reactions were seen in all three series: presumably these antisera contained both antiglobulin and antialbumin.…”

Section: Introductionmentioning

confidence: 99%

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The Demonstration of the Increase of Globulin in Diphtheria Antitoxins by the Precipitation Reaction

Taylor

1935

J. Hyg.

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1. By titration against horse serum and its protein fractions anti-horse sera can be divided into three groups, according to the antibodies responsible for the main or earliest particulating zone, namely the sera containing antibodies against (a) globulin only, (b) albumin only, and (c) both globulin and albumin.2. For the quantitative estimation of a protein fraction in a horse serum it is necessary to use an antiserum containing a main zone antibody acting with that protein only.This is illustrated in experiments, designed to show the increased globulin content of diphtheria antitoxin, in which antisera containing antibodies against both globulin and albumin failed to register an increase in the globulin content.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Demonstration of the Increase of Globulin in Diphtheria Antitoxins by the Precipitation Reaction

Taylor

1935

J. Hyg.

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“…The concentrations determined refractometrically were: euglobulin 3-2 %/, pseudoglobulin 1.7 %. The precipitin contents of these globulin preparations were determined by the Dean & Webb (1926) optimal proportions method. In agreement with Adair & Taylor (1936), the antibody content of the euglobulin solution (optimal ratio 1: 27) was found to be higher than that of the pseudoglobulin (1 : 60).…”

Section: Introductionmentioning

confidence: 99%

Reversed passive anaphylaxis in the guinea-pig

Ende

1940

Epidemiol. Infect.

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Attempts to demonstrate reversed passive anaphylaxis in the guinea-pig with crystalline egg albumin as sensitizing antigen have been uniformly negative.When purified anti-pneumococcal antibody globulin was used as sensitizing antigen, reversed anaphylactic shock could be elicited in guinea-pigs by the intravenous injection of precipitins for the antibody globulin.The mild reactions which could be elicited when the total globulins from the serum of normal rabbits were used as sensitizing antigen are probably dependent on the presence of small amounts of y globulin.Reversed passive anaphylaxis, like direct anaphylaxis, is dependent on a cellular mechanism, and the success of experiments in which rabbit antibody globulin was used as sensitizing antigen depends on the acceptability of the antibody to the cells of the guinea-pig's tissues.Antigenic differences between antibody globulins and total normal globulins from rabbit serum are noted.

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“…Its purpose was to determine the relative parts played in the reaction by the virus elementary body and the soluble antigen, and to find out whether any specific differences could be demonstrated between different strains of influenza virus A. Almost all the work has been done by means of 'chess-board' experiments of the type first used by Dean & Webb (1926) in their classical work on the complementfixation reaction. These experiments, in which a series of antigen dilutions is tested against a series of serum dilutions with a constant dose of complement, convey very much more information about the nature of a complement-fixation reaction than is obtainable by a simple titration of antigen or serum.…”

mentioning

confidence: 99%

An analysis of the complement-fixation reaction in influenza

Hoyle¹

1945

J. Hyg.

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This paper describes a study of the mechanism of the complement-fixation reaction in influenza. Its purpose was to determine the relative parts played in the reaction by the virus elementary body and the soluble antigen, and to find out whether any specific differences could be demonstrated between different strains of influenza virus A. Almost all the work has been done by means of 'chess-board' experiments of the type first used by Dean & Webb (1926) in their classical work on the complementfixation reaction. These experiments, in which a series of antigen dilutions is tested against a series of serum dilutions with a constant dose of complement, convey very much more information about the nature of a complement-fixation reaction than is obtainable by a simple titration of antigen or serum.standards of 0, 25, 50, 75 and 100% haemolysis was prepared and the tubes matched against these.A constant dose of complement (21 M.H.D.) has been used in most of the experiments. The complement was the pooled serum of several guinea-pigs preserved by the boric acid-sorbite-azide method of Richardson (1941). This preserved liquid complement has been found very satisfactory as its titre remains unchanged for weeks and enables a large number of experiments to be done with the same batch of complement.The sera used in this work were mainly human convalescent sera from the epidemic of 1943-4. They were inactivated at 560C. for 30 min. and preserved by addition of 0-08 % sodium azide. Some of the antigens used in the work have also been preserved by sodium azide.

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The influence of optimal proportions of antigen and antibody in the serum precipitation reaction

Cited by 87 publications

References 4 publications

The Demonstration of the Increase of Globulin in Diphtheria Antitoxins by the Precipitation Reaction

The Demonstration of the Increase of Globulin in Diphtheria Antitoxins by the Precipitation Reaction

Reversed passive anaphylaxis in the guinea-pig

An analysis of the complement-fixation reaction in influenza

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