The influence of protein adsorption on nanoparticle association with cultured endothelial cells

The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle

“…As reported previously, the protein corona on nanoparticles would critically impact the biological identities of nanoparticles [6][7][8][9] and/or result in the physiological and pathological consequences of the macrophage uptake, blood coagulation, complement activation and cellular toxicity, etc. [10][11][12][13][14][15][16]. The elaborate investigation on protein corona would accordingly make great sense for the extensive application of nanomaterials.…”

Interaction of metal oxide nanoparticles with lung surfactant protein A

“…We have previously demonstrated that the addition of Fetal Calf Serum leads to deagglomeration [1] and hence to smaller particle sizes for some of the particles tested here. Also, it could be demonstrated by Ehrenberg and coworkers that particles coated with serum proteins adsorbed onto endothelial cells in higher amounts as uncoated ones [2]. Hence, the phenomenon of protein adsorption onto nanoparticles entering biological systems could lead to significant toxicological consequences and must be investigated thoroughly.…”

Interaction of curcumin nanoformulations with human plasma proteins and erythrocytes

“…In general, serum proteins readily bind onto the surfaces of various nanoparticles within one hour. 47,48 Proteins adsorb on the surfaces of nanoparticles quickly and their characteristics may change before reaching the intended target(s). The degree of protein interaction may depend on particle size, surface stabilization, functional or chemical groups on the surface, and composition of the particles.…”