The influence of strontium release rate from bioactive phosphate glasses on osteogenic differentiation of human mesenchymal stem cells

Štefanič, Martin; Peroglio, Marianna; Stanciuc, Ana-Maria; Machado, Gil Costa; Campbell, Ian; Kržmanc, Marjeta Maček; Alini, Mauro; Zhang, X.

doi:10.1016/j.jeurceramsoc.2017.08.005

Cited by 24 publications

(15 citation statements)

References 43 publications

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“…This suggests that Ca 2+ release is less affected by the decrease in glass solubility caused by Sr 2+ addition. Similar results were obtained using strontiumdoped phosphate-based glasses prepared via MQ (Stefanic et al, 2018). Ca 2+ release was found to be independent of strontium loading; its release over time was very similar for systems containing 1, 5, and 10 mol% of SrO.…”

Section: Resultssupporting

confidence: 80%

“…These studies confirmed the potential applications of Sr-doped PGs as effective vehicles to deliver Sr 2+ to bone cells (Abou Neel et al, 2009a;Lakhkar et al, 2011). These glasses can also promote osteogenic differentiation of human mesenchymal stem cells that make them a great choice in various orthopedic, dental, and maxillofacial applications (Stefanic et al, 2018).…”

Section: Introductionsupporting

confidence: 63%

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Mesoporous Strontium-Doped Phosphate-Based Sol-Gel Glasses for Biomedical Applications

et al. 2020

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Mesoporous phosphate-based glasses have great potential as biomedical materials being able to simultaneously induce tissue regeneration and controlled release of therapeutic molecules. In the present study, a series of mesoporous phosphate-based glasses in the P 2 O 5-CaO-Na 2 O system, doped with 1, 3, and 5 mol% of Sr 2+ , were prepared using the sol-gel method combined with supramolecular templating. A sample without strontium addition was prepared for comparison. The non-ionic triblock copolymer EO 20 PO 70 EO 20 (P123) was used as a templating agent. Scanning electron microscopy (SEM) images revealed that all synthesized glasses have an extended porous structure. This was confirmed by N 2 adsorption-desorption analysis at 77 K that shows a porosity typical of mesoporous materials. 31 P magic angle spinning nuclear magnetic resonance (31 P MAS-NMR) and Fourier transform infrared (FTIR) spectroscopies have shown that the glasses are mainly formed by Q 1 and Q 2 phosphate groups. Degradation of the glasses in deionized water assessed over a 7-day period shows that phosphate, Ca 2+ , Na + , and Sr 2+ ions can be released in a controlled manner over time. In particular, a direct correlation between strontium content and degradation rate was observed. This study shows that Sr-doped mesoporous phosphate-based glasses have great potential in bone tissue regeneration as materials for controlled delivery of therapeutic ions.

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Section: Resultssupporting

confidence: 80%

Section: Introductionsupporting

confidence: 63%

Mesoporous Strontium-Doped Phosphate-Based Sol-Gel Glasses for Biomedical Applications

et al. 2020

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“…After two and three weeks in culture, there was no significant change between the cells treated with particles or their dissolution products and the control. These results were consistent with recent research that reported that Sr containing BGs could enhance the osteogenic differentiation of MSCs [51,109,110].…”

Section: Osteogenic Differentiation On Exposure To the Nanoparticlessupporting

confidence: 93%

“…The effect of Bioglass and its dissolution products on stem cells is less clear and there is some contradictory evidence in the literature. Osteogenic differentiation of human bone marrow stem cells (hMSCs) in contact with BGs was investigated [47][48][49][50][51][52][53], but results varied depending on the particular types of stem cells tested. Some articles found no effect on stem cells in vitro: Bioglass 45S5 did not stimulate the alkaline phosphatase (ALP) activity of hMSCs from five different human donors [54] and a Bioglass 45S5 coating on a polymeric substrate had no direct effect on osteoprogenitor cell differentiation or bone formation [55].…”

Section: Introductionmentioning

confidence: 99%

Human mesenchymal stem cells differentiate into an osteogenic lineage in presence of strontium containing bioactive glass nanoparticles

Naruphontjirakul

Tsigkou

et al. 2019

Acta Biomaterialia

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“…The 2B6Sr composition is substations of two-thirds of SiO 2 with B 2 O 3 and three quarters of MgO with SrO in 13-93 composition while keeping the rest oxides the same. As a trace element in the human body, Sr is reported to accumulate in bone and replace calcium in metabolic process [19], and to stimulate cell proliferation and differentiation [20][21][22][23][24]. Both B 2 O 3 and SrO have been reported to stimulate bone ingrowth in recent studies [20][21][22][23][24][25][26].…”

Section: Discussionmentioning

confidence: 99%

Cellular Response to 3‐D Printed Bioactive Silicate and Borosilicate Glass Scaffolds

et al. 2018

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The repair and regeneration of loaded segmental bone defects is a challenge for both materials and biomedical science communities. Our recent work demonstrated the capability of bioactive glass in supporting bone healing and defect bridging using a rabbit femur segmental defect model without growth factors or bone marrow stromal cells (BMSCs). Here in the current work, a comprehensive in vitro evaluation of bioactive silicate (13-93) and borosilicate (2B6Sr) glass scaffolds was conducted to provide further understanding of their biological performances and to establish a correlation between in vitro and in vivo behaviors. Our in vitro evaluation using a murine MC3T3-E1 cell line confirmed the capability of both scaffolds to support cell attachment, vascular endothelial growth factor (VEGF) formation, and to stimulate mineral deposition and osteoblast marker gene expression. In particular, borosilicate (2B6Sr) glass showed a better capability in supporting the mineralization and gene expression than silicate (13-93) glass, consistent with a faster bone healing ability in vivo. The current in vitro results, combined with our previous in vivo findings, provide a strong basis for the further translational evaluation of bioactive glass scaffolds and for potential preclinical practice. © 2018 Wiley Periodicals, Inc. J. Biomed. Mater. Res. Part B, 2018.

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The influence of strontium release rate from bioactive phosphate glasses on osteogenic differentiation of human mesenchymal stem cells

Cited by 24 publications

References 43 publications

Mesoporous Strontium-Doped Phosphate-Based Sol-Gel Glasses for Biomedical Applications

Mesoporous Strontium-Doped Phosphate-Based Sol-Gel Glasses for Biomedical Applications

Human mesenchymal stem cells differentiate into an osteogenic lineage in presence of strontium containing bioactive glass nanoparticles

Cellular Response to 3‐D Printed Bioactive Silicate and Borosilicate Glass Scaffolds

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