The interleukin-10 inducing effect of transforming growth factor-β on human naive CD4+ T cells from cord blood is restricted to the TH1 subset

Kapitein, Berber; Tiemessen, Machteld M.; Liu, Wai M.; Dijk, Adrie G. Van Ieperen-Van; Hoekstra, M. O.; Hoffen, Els van; Knol, Edward F.

doi:10.1111/j.1365-2249.2006.03282.x

Cited by 2 publications

(3 citation statements)

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“…However, due to the lack of specific surface markers and transcription factors they remained for a long time an enigmatic population, and in humans, they were mainly identified after in vitro culture according to IL-10 production [460]. However, a caveat of this approach is that IL-10 is not exclusively produced by regulatory T-cells, and that it can be acquired or lost in culture [461][462][463]. It is therefore critical to track Tr1-like cells directly ex vivo, with no or minimal in vitro manipulation.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, IL-10 production in human CD4 + T-cells has a complex regulation, and may require peculiar stimulation conditions. Naïve helper T-cells are devoid of IL-10 producing capacities, and start to produce IL-10 following TCR stimulation in the presence of permissive cytokines only after several days [ 462 , 473 ]. Conversely, Ag-experienced CD45RO + memory T-cells possess significant IL-10 producing capacities ex vivo.…”

Section: Introductionmentioning

confidence: 99%

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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

et al. 2021

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The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

et al. 2021

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“…TME consists of diverse cells comprising not only tumor cells but also stromal cells, immune cells such as macrophages, and lymphoid cells (42). Immune cells play an important role in TME by being involved in immunosuppression, invasion, metastasis, and angiogenesis via cell-to-cell communication, activation of molecular crosstalk, and production of various growth factors and chemokines (43). To indirectly confirm the effect of M-EVs derived from the immune cells constituting the TME, we generated an experimental setup similar to the TME by using a transwell plate.…”

Section: Discussionmentioning

confidence: 99%

Influence of Canine Macrophage-derived Extracellular Vesicles on Apoptosis in Canine Melanoma and Osteosarcoma Cell Lines

Lee

Yang

et al. 2021

Anticancer Res

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Background/Aim: The purpose of this study was to evaluate the effect of extracellular vesicles derived from canine M1-polarized macrophages (M1EVs) on canine tumor cells, such as D17 (osteosarcoma cells) and LMeC (melanoma cells). Materials and Methods: Protein expression was determined by western blot analysis. Gene expression was determined by RT-qPCR. In addition, cell apoptosis was analyzed by Annexin V/PI staining. Results: In the case of M1EV, the levels of pro-inflammatory cytokines such as TNFα, IL-6 and IL-1β were increased, and nitrate/nitrite levels were also increased. M1EV induced apoptosis of tumor cells by increasing caspase-3 and caspase-7 activation. In addition, M1EVs decreased expression of CCR4, Foxp3 and CTLA-4 in canine peripheral mononuclear cells cocultured with tumor cells. Conclusion: M1EV could be an effective anti-cancer therapeutic approach in melanoma and osteosarcoma and M1EVs can be used as immunomodulators in the tumor microenvironment for cancer treatment.Cancer is a leading cause of death in companion animals, with mortality rates of approximately 15~30% in dogs (1). Cancers can develop in various dog organs, including the bone (osteosarcoma) and skin/epithelial tissues (melanoma)(2). Osteosarcoma is one of the most malignant tumors affecting the bones in dogs. Appendicular osteosarcoma is the most common form of osteosarcoma in dogs, and accounts for up to 75~85% of all osteosarcoma lesions. This type of cancer is highly aggressive and metastatic, and pulmonary metastasis is common (3). Various modalities have been used to treat osteosarcoma in dogs, including surgical removal, chemotherapy, and radiation therapy, and the median survival time is approximately 103~175 days with surgery and 235~366 days when surgery is combined with chemotherapy (4). Similarly, melanoma is one of the most malignant tumors in dogs, which usually develops in the oral cavity and acral sites (5). Clinical management of melanoma includes surgical resection, radiation therapy, and chemotherapy; however, because of the highly aggressive and metastatic biological behavior of this tumor, controlling the disease using these therapies remains a challenge (6).The tumor microenvironment (TME) is a complex and heterogenous collection of tissues that comprises not only neoplastic cells, but also other diverse immune cells, such as lymphocytes, macrophages, and neutrophils, and this tumorimmune cell cross-talk results in a rich milieu of cytokines and growth factors that play an important role in tumor progression, advancement of inflammation, and angiogenesis (7). Among these immune cells, tumor-associated macrophages (TAMs) represent the major component. These macrophages are mainly classified in two polarization types: 1) the classically activated type 1 macrophage (M1), which produces high levels of proinflammatory cytokines and shows enhanced phagocytosis, and 2) the alternatively activated type 2 macrophage (M2), which facilitates the resolution of inflammation and promotes tissue repair and angiogenesis (...

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The interleukin-10 inducing effect of transforming growth factor-β on human naive CD4+ T cells from cord blood is restricted to the TH1 subset

Cited by 2 publications

References 26 publications

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Influence of Canine Macrophage-derived Extracellular Vesicles on Apoptosis in Canine Melanoma and Osteosarcoma Cell Lines

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