This article aims to analyze the correlations between the level of religiosity and emotional responses to artificial intelligence (AI). In these times marked by the growing penetration of artificial intelligence into people’s everyday lives, it is important to identify the sociocultural determinants of attitudes towards this increasingly dynamically developing technology. A significant element of society’s cultural mosaic is religiosity, and, as such, it undoubtedly has an effect on emotional responses to AI. This is a reason to investigate how religions and religiosity impact attitudes towards AI. The study used Welch’s analysis of variance (ANOVA) to compare university students’ emotional reactions to AI across religiosity levels, which allowed for identifying significant differences in the levels of emotions such as fear, sadness, and anger between irreligious, religious, and indifferent groups. Significant differences in emotional responses to AI were found between individuals with different religiosity levels. Compared to irreligious and indifferent students, the religious ones more often showed intense emotions, such as fear and anger. The results indicate the need to include the issues of religion and religiosity in the process of designing and implementing AI. This may contribute to a greater acceptance of this technology in a society of individuals with different levels of religiosity. Understanding the effect of religiosity on the response to AI is also of considerable importance for the development and implementation of ethically responsible AI solutions, which should take account of the diversity of individuals’ beliefs and value systems.