The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly Electrodeposited Graphene on Choline&amp;ndash;Gold Nanoparticals

He, Yaping; Yang, Xiaohui; Han, Qian; Zheng, Jianbin

doi:10.20944/preprints201704.0188.v1

Cited by 2 publications

(1 citation statement)

References 51 publications

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“…Different methods including spectrophotometric assays, TLC bioautographic assays, high performance liquid chromatography, electrophoresis, isotope assays, enzyme-linked immunosorbent assays and electrochemical techniques have been reported for both the qualitative and quantitative measurement of tyrosinase activity [ 6 , 17 ]. Among these assay methods, electrochemical measurements are affordable, reliable and robust tools for measuring the antioxidant capacity of plant extracts [ 18 ] and have also been applied in cosmetic formulations and the detection of phenolic compounds in extracts and wines [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Screening and Evaluation of Lamiaceae Plant Species from South Africa with Potential Tyrosinase Activity

Etsassala

Popoola

Adeloye

et al. 2019

Sensors

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South Africa is a country with a wide variety of plants that may contain excellent anti-tyrosinase inhibitors. With wide applications in cosmetics, pharmaceuticals and food products, tyrosinase inhibitors have received very special attention in the recent past as a way of preventing the overproduction of melanin in epidermal layers which often over time brings detrimental effects on human skin. In this present study, a fast screening method using a cyclic voltammetry technique was applied in the evaluation of methanolic extracts of twenty-five species of plants from the Lamiaceae family for anti-tyrosinase activity. Among these plants, those that showed a fast current inhibition rate at a minimum concentration when compared to a kojic acid standard were classified as having the greatest anti-tyrosinase activity. These include Salvia chamelaeagnea, S. dolomitica, Plectranthus ecklonii, P. namaensis, and P. zuluensis. The results presented herein focused in particular on providng firsthand information for further extensive research and exploration of natural product materials with anti-tyrosinase activity from South African flora for use in cosmetics, skin care and medicinal treatments.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Screening and Evaluation of Lamiaceae Plant Species from South Africa with Potential Tyrosinase Activity

Etsassala

Popoola

Adeloye

et al. 2019

Sensors

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Development of a Novel Biosensor Based on Tyrosinase/Platinum Nanoparticles/Chitosan/Graphene Nanostructured Layer with Applicability in Bioanalysis

Apetrei

2019

Materials

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The present paper describes the preparation and characterization of a graphene, chitosan, platinum nanoparticles and tyrosinase-based bionanocomposite film deposited on the surface of a screen-printed carbon electrode for the detection of L-tyrosine by voltammetry. The redox process on the biosensor surface is associated with the enzymatic oxidation of L-tyrosine, which is favoured by graphene and platinum nanoparticles that increase electrical conductivity and the electron transfer rate. Chitosan ensures the biocompatibility between the tyrosinase enzyme and the solid matrix, as well as a series of complex interactions for an efficient immobilization of the biocatalyst. Experimental conditions were optimized so that the analytical performances of the biosensor were maximal for L-tyrosine detection. By using square wave voltammetry as the detection method, a very low detection limit (4.75 × 10−8 M), a vast linearity domain (0.1–100 μM) and a high affinity of the enzyme for the substrate (KMapp is 53.4 μM) were obtained. The repeatability of the voltammetric response, the stability, and the reduced interference of the chemical species present in the sample prove that this biosensor is an excellent tool to be used in bioanalysis. L-tyrosine detection in medical and pharmaceutical samples was performed with very good results, the analytical recovery values obtained being between 99.5% and 101%. The analytical method based on biosensor was validated by the standard method of analysis, the differences observed being statistically insignificant at the 99% confidence level.

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The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly Electrodeposited Graphene on Choline–Gold Nanoparticals

Cited by 2 publications

References 51 publications

Electrochemical Screening and Evaluation of Lamiaceae Plant Species from South Africa with Potential Tyrosinase Activity

Electrochemical Screening and Evaluation of Lamiaceae Plant Species from South Africa with Potential Tyrosinase Activity

Development of a Novel Biosensor Based on Tyrosinase/Platinum Nanoparticles/Chitosan/Graphene Nanostructured Layer with Applicability in Bioanalysis

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