The investigation of miR-221-3p and PAK1 gene expressions in breast cancer cell lines

Ergün, Sercan; Tayeb, Tayeb Sadiq; Arslan, Ahmet; Temiz, Ebru; Arman, Kaifee; Safdar, Muhammad; Dağlı, Hasan; Korkmaz, Murat; Nacarkahya, Gülper; Kirkbes, Sevil; Öztuzcu, Serdar

doi:10.1016/j.gene.2014.11.036

Cited by 23 publications

(13 citation statements)

References 22 publications

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“…Dysregulation of miR-150 has been demonstrated in a number of solid tumors (reviewed in [ 55 ]). Additionally, miR-485, miR-34c, miR-221, or miR-193a and miRNAs from the mir-10 or let-7 families identified as deregulated in HPV-negative tumors in our study participate in the regulation of proliferation, apoptosis, and invasion, and have been proposed as a prognostic indicators in patients with solid cancers [ 56 – 60 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the miRNA profiles in HPV-positive and HPV-negative tonsillar tumors and a model system of human keratinocyte clones

et al. 2016

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BackgroundBetter insights into the molecular changes involved in virus-associated and -independent head and neck cancer may advance our knowledge of HNC carcinogenesis and identify critical disease biomarkers. Here we aimed to characterize the expression profiles in a matched set of well-characterized HPV-dependent and HPV-independent tonsillar tumors and equivalent immortalized keratinocyte clones to define potential and clinically relevant biomarkers of HNC of different etiology.MethodsFresh frozen tonsillar cancer tissues were analyzed together with non-malignant tonsillar tissues and compared with cervical tumors and normal cervical tissues. Furthermore, relative miRNAs abundance levels of primary and immortalized human keratinocyte clones were evaluated. The global quantitation of miRNA gene abundance was performed using a TaqMan Low Density Array system. The confirmation of differentially expressed miRNAs was performed on a set of formalin-fixed paraffin-embedded tumor samples enriched for the tumor cell fraction by macrodissection.ResultsWe defined 46 upregulated and 31 downregulated miRNAs characteristic for the HPV-positive tonsillar tumors and 42 upregulated miRNAs and 42 downregulated miRNAs characteristic for HPV-independent tumors. In comparison with the expression profiles in cervical tumors, we defined miR-141-3p, miR-15b-5p, miR-200a-3p, miR-302c-3p, and miR-9-5p as specific for HPV induced malignancies. MiR-335-5p, miR-579-3p, and miR-126-5p were shared by the expression profiles of HPV-positive tonsillar tumors and of the HPV immortalized keratinocyte clones, whereas miR-328-3p, miR-34c-3p, and miR-885-5p were shared by the miRNA profiles of HPV-negative tonsillar tumors and the HPV-negative keratinocytes.ConclusionsWe identified the miRNAs characteristic for HPV-induced tumors and tonsillar tumors of different etiology, and the results were compared with those of the model system. Our report presents the basis for further investigations leading to the identification of clinically relevant diagnostic and/or therapeutic biomarkers for tumors of viral and non-viral etiology.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-016-2430-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparison of the miRNA profiles in HPV-positive and HPV-negative tonsillar tumors and a model system of human keratinocyte clones

et al. 2016

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“…Hence, this study focused attention on the BMMSC MVs-derived miR-221-3p. Enormous studies have revealed that miR-221-3p is aberrantly expressed in various cancers and participate in the regulation of tumorigenesis and development, like cervical squamous carcinoma (Wu et al, 2019), hepatocellular carcinoma (Li et al, 2019), medulloblastoma (Yang et al, 2019), and breast cancer (Ergun et al, 2015). However, the role of miR-221-3p in AML has not been reported.…”

Section: Discussionmentioning

confidence: 99%

miR-221-3p Delivered by BMMSC-Derived Microvesicles Promotes the Development of Acute Myelocytic Leukemia

Zhang

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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The study aims to investigate the effects of miR-221-3p in bone marrow mesenchymal stem cell (BMMSC)-derived microvesicles (MVs) on cell cycle, proliferation and invasion of acute myelocytic leukemia (AML). Methods: Bioinformatics was used to predict differentially expressed miRNAs (DEmiRNAs) in AML. The morphology of BMMSC-derived MVs was observed under an electron microscope, and the positional relation of MVs and OCI-AML2 cells was observed by a fluorescence microscope. MTT, Transwell, and flow cytometry assays were used to analyze the effects of MVs on OCI-AML2 cells. The targeted relationship between miR-221-3p and CDKN1C was detected by dual luciferase assay. Results: It was verified that miR-221-3p promoted the proliferation, invasion and migration of OCI-AML2 cells, and induced the cell cycle arrest in G1/S phase as well as inhibited cell apoptosis. Further studies showed that MVs promoted the proliferation, migration and invasion of AML, and induced the cell cycle arrest in G1/S phase through miR-221-3p. It was confirmed that miR-221-3p can directly target CDKN1C to regulate cell cycle, proliferation and invasion of AML. Conclusion: miR-221-3p in BMMSC-derived MVs regulated AML cell cycle, cell proliferation and invasion through targeting CDKN1C. miR-221-3p and CDKN1C were considered to be potential targets and biomarkers for the treatment of AML in clinic.

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“…Previous reports have shown that miR-221-3p plays a key role in abnormal cell proliferation and differentiation in cancer [19,21,25]. Wu et al reports that miR-221-3p directly targets ART4 to regulate epithelial ovarian cancer [22].…”

Section: Discussionmentioning

confidence: 99%

“…20 Emerging studies indicate that miR-221-3p functions as a key mediator of invasion and metastasis of various cancers, such as colon cancer, epithelial ovarian cancer, gastric carcinoma, prostate cancer, breast cancer and pancreatic cancer. [21][22][23][24][25][26] Therefore, miR-221-3p was suggested to be a potential target for cancer prognosis and therapeutics.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-221-3p promotes proliferation and invasion in non-small cell lung cancer via targeting Axin2 to regulate Wnt/β-catenin signaling pathway

Zheng

Liu²,

et al. 2020

Preprint

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ObjectiveThe mortality rate of lung cancer ranks first in malignant tumors. Among them, non-small cell lung cancer (NSCLC) accounts for about 85% of all lung cancer patients. In this study, we explore part of the mechanism of development and progression of NSCLC.Methods/ ResultsFirstly, there was an increase in microRNA-221-3p (miR-221-3p) expression and a decrease in Axin2 expression in NSCLC tissues using real-time reverse transcription polymerase chain reaction. Further studies showed that miR-221-3p inhibited the expression of Axin2, which negatively regulated the Wnt signaling pathway. With the method of inhibiting and overexpressing the expression of miR-221-3p and/or Axin2 respectively in NSCLC cell lines A549 and H1975, we found that inhibiting the expression of miR-221-3p leaded to a decrease in cell proliferation, migration and invasion, just like the results of overexpressing Axin2. Relatively speaking, overexpression of miR-221-3P in NSCLC cell lines showed the increase of proliferation as well as the decrease of apoptosis. Thus, we knew that miR-221-3p promoted the migration and invasion of NSCLC cells in vitro. What’s more, according to western blot and EdU assay, we demonstrated that overexpression of miR-221-3p inhibited the expression of Axin2 and subsequently activate classical Wnt/β-catenin signaling pathway. At last, a series of methods were used to identify that miR-221-3p inhibited Axin2 expression, increased cell proliferation, invasion and migration, and decreased cell apoptosis.ConclusionOur results suggest that miR-221-3p inhibits the expression of Axin2 and indirectly activates the typical Wnt/β-catenin signaling pathway, thus promoting tumor proliferation and invasion in NSCLC.

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The investigation of miR-221-3p and PAK1 gene expressions in breast cancer cell lines

Cited by 23 publications

References 22 publications

Comparison of the miRNA profiles in HPV-positive and HPV-negative tonsillar tumors and a model system of human keratinocyte clones

Comparison of the miRNA profiles in HPV-positive and HPV-negative tonsillar tumors and a model system of human keratinocyte clones

miR-221-3p Delivered by BMMSC-Derived Microvesicles Promotes the Development of Acute Myelocytic Leukemia

MicroRNA-221-3p promotes proliferation and invasion in non-small cell lung cancer via targeting Axin2 to regulate Wnt/β-catenin signaling pathway

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