The involvement of PUMP from mitochondria of Araucaria angustifolia embryogenic cells in response to cold stress

Valente, Caroline; Pasqualim, P.; Jacomasso, Thiago; Maurer, Juliana Bello Baron; Souza, Emanuel Maltempi de; Martinez, Gláucia Regina; Rocha, Maria Eliane Merlin; Carnieri, Eva Gunilla Skare; Cadena, Sílvia Maria Suter Correia

doi:10.1016/j.plantsci.2012.09.007

Cited by 10 publications

(7 citation statements)

References 38 publications

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“…3B). The AOX capacities in mitochondria isolated from cell cultures of the possibly nonthermogenic gymnosperm A. angustifolia (Mariano et al, 2008;Valente et al, 2012) were similar to those in C. revoluta microsporangia obtained in the current study ( Fig. 3B; Table 4).…”

Section: Aox Respiration Via Craox1 In Microsporophylls Plays a Rolsupporting

confidence: 81%

“…In thermogenic gymnosperms, the presence of cyanide-resistant respiration was initially demonstrated in a respiratory assay using sporophylls (microsporophylls) of the male cones of Zamia pumila (Zamiaceae; Skubatz et al, 1993). However, AOX respiration was also confirmed in nonthermogenic gymnosperms, such as white spruce (Picea glauca; Johnson-Flanagan and Owens, 1986;Weger and Guy, 1991) and Araucaria angustifolia (Mariano et al, 2008;Valente et al, 2012). In addition, since the first report of molecular evidence for the existence of AOX in Pinus pinea (Frederico et al, 2009), high-throughput sequencing has revealed several deduced AOX protein sequences in gymnosperms (Neimanis et al, 2013).…”

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confidence: 99%

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Alternative Oxidase Capacity of Mitochondria in Microsporophylls May Function in Cycad Thermogenesis

Ito‐Inaba

Sato

et al. 2019

Plant Physiol.

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Cone thermogenesis is a widespread phenomenon in cycads and may function to promote volatile emissions that affect pollinator behavior. Given their large population size and intense and durable heat-producing effects, cycads are important organisms for comprehensive studies of plant thermogenesis. However, knowledge of mitochondrial morphology and function in cone thermogenesis is limited. Therefore, we investigated these mitochondrial properties in the thermogenic cycad species Cycas revoluta. Male cones generated heat even in cool weather conditions. Female cones produced heat, but to a lesser extent than male cones. Ultrastructural analyses of the two major tissues of male cones, microsporophylls and microsporangia, revealed the existence of a population of mitochondria with a distinct morphology in the microsporophylls. In these cells, we observed large mitochondria (cross-sectional area of 2 mm 2 or more) with a uniform matrix density that occupied .10% of the total mitochondrial volume. Despite the size difference, many nonlarge mitochondria (cross-sectional area ,2 mm 2 ) also exhibited a shape and a matrix density similar to those of large mitochondria. Alternative oxidase (AOX) capacity and expression levels in microsporophylls were much higher than those in microsporangia. The AOX genes expressed in male cones revealed two different AOX complementary DNA sequences: CrAOX1 and CrAOX2. The expression level of CrAOX1 mRNA in the microsporophylls was 100 times greater than that of CrAOX2 mRNA. Collectively, these results suggest that distinctive mitochondrial morphology and CrAOX1-mediated respiration in microsporophylls might play a role in cycad cone thermogenesis.

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Section: Aox Respiration Via Craox1 In Microsporophylls Plays a Rolsupporting

confidence: 81%

mentioning

confidence: 99%

Alternative Oxidase Capacity of Mitochondria in Microsporophylls May Function in Cycad Thermogenesis

Ito‐Inaba

Sato

et al. 2019

Plant Physiol.

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“…Cell viability was determined by fluorescence microscopy using fluorescein diacetate (FDA; Molecular Probes, Eugene, OR) plus propidium iodide (PI) stain (Valente et al ). Due to its non‐polar characteristics, FDA enters the cells and becomes fluorescent when hydrolyzed by endogenous esterase of viable cells.…”

Section: Methodsmentioning

confidence: 99%

“…The respiration of Araucaria cells was measured as described by Valente et al (), with modifications. Briefly, the oxygen uptake was determined by high‐resolution respirometry with an Oxygraph‐2 k (OROBOROS Instruments, Innsbruck, Austria) at 28°C under gentle agitation.…”

Section: Methodsmentioning

confidence: 99%

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Short‐term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells

Furlanetto

Cadena

Martinez

et al. 2018

Physiologia Plantarum

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We evaluated the effect of global warming on Araucaria angustifolia (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40% after incubation at 30 and 37°C for 24 and 6 h, respectively, while 2 h at 40 and 42°C killed 95% of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2 h at 42°C. The in vitro activity of enzymes of the base excision repair (BER) pathway was determined. Apurinic/apyrimidine endonuclease, measured in extracts from cells incubated for 2 h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30-min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited at 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and 37°C, and that they are all very sensitive to 40 or 42°C. Thus, A. angustifolia will likely be vulnerable to the short-term high temperature events associated with global warming.

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Defense Response in Chickpea Pod Wall due to Simulated Herbivory Unfolds Differential Proteome Profile

et al. 2020

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The involvement of PUMP from mitochondria of Araucaria angustifolia embryogenic cells in response to cold stress

Cited by 10 publications

References 38 publications

Alternative Oxidase Capacity of Mitochondria in Microsporophylls May Function in Cycad Thermogenesis

Alternative Oxidase Capacity of Mitochondria in Microsporophylls May Function in Cycad Thermogenesis

Short‐term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells

Defense Response in Chickpea Pod Wall due to Simulated Herbivory Unfolds Differential Proteome Profile

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