The isoflavone genistein inhibits copper and peroxyl radical mediated low density lipoprotein oxidation in vitro

Antioxidative Activities of Galloyl Glucopyranosides from the Stem-Bark ofJuglans mandshurica

“…Blood was drawn from healthy normolipidemic volunteers, and human LDL was prepared from plasma by sequential flotation ultracentrifugation as previously described. 20,21) For the Cu 2þ -mediated oxidation experiments, LDL was dialyzed for 24 h at 4 C against EDTA-free, phosphatebuffered saline (PBS) to remove EDTA. For the azoinitiated oxidation experiments, LDL was dialyzed overnight against PBS containing 1 mM EDTA.…”

“…Copper ioninduced LDL oxidation was measured as previously described. 20,21) Briefly, a test compound was pre-incubated with LDL (100 mg/ml) in PBS (10 mM, pH 7.4, final volume of 1 ml), and oxidation was initiated by adding 5 mM of CuSO 4 at 37 C. After incubating for 3 h, the reaction was terminated by adding of 1 mM EDTA and 500 mM butylated hydroxy toluene (BHT). The amount of LDL oxidized to malondialdehyde (MDA) was measured by the thiobarbituric acid reactive substances (TBARS) assay.…”

“…LDL oxidation mediated by an azo compound was determined as previously described. 20,21) Briefly, LDL (100 mg/ml) in PBS (pH 7.4, final volume of 1 ml) was pre-incubated with a test compound, and oxidation was initiated by adding 5 mM aqueous AAPH at 37 C for 3 h. The reaction was stopped by adding 500 mM BHT. The extent of LDL oxidation was quantified by TBARS generation.…”

Inhibitory Effect of Coumarins from Weigela subsessilis on Low Density Lipoprotein Oxidation

“…LDL Preparation Blood was drawn from healthy normolipidemic volunteers and human LDL was prepared from plasma by sequential flotation ultracentrifugation as described previously. [10][11][12] For Cu 2ϩ -mediated oxidation experiments, LDL was dialysed for 20 h at 4°C against EDTA-free, phosphate buffered saline (PBS) to remove EDTA. 11,12) For azo-initiated oxidation experiments, LDL was dialysed overnight against the same PBS containing 1 mM EDTA.…”

Effect of Bean Extract of Yabumame (<i>Amphicarpaea bracteata</i> (L.) Fernald subsp. <i>edgeworthii</i> (Benth.) H.Ohashi) on Low-Density Lipoprotein Oxidation <i>In Vitro</i>

“…Several research groups have reported that soybean extract exerts antioxidant activity and that phenolic compounds may contribute to this activity (Georgetti et al, 2006;Malencic et al, 2008). Soybean isoflavones such as daidzein and genistein can inhibit LDL oxidation by breaking the chain reaction of lipid oxidation through scavenging ROS (Kerry and Abbey, 1998;Lee et al, 2005). In addition, the antioxidant activity of black soybean against LDL oxidation was stronger than that of yellow soybean (Astadi et al, 2009;Takahashi et al, 2005).…”