The Isoflavone-Rich Fraction of the Crude Extract of the Puerariae Flower Increases Oxygen Consumption and BAT UCP1 Expression in High-Fat Diet-Fed Mice

Kamiya, Tomoyasu; Nagamine, Rika; Sameshima-Kamiya, Mayu; Tsubata, Masahito; Ikeguchi, Motoya; Takagaki, Kinya

doi:10.5539/gjhs.v4n5p147

Cited by 21 publications

(31 citation statements)

References 32 publications

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“…High-fat diets are widely used to induce obesity in animal experiments (Kamiya et al, 2012;Maeda et al, 2009;Nishi et al, 2007;Yamasaki et al, 2013). The high-fat diet experimental period in the present study induced a marked weight increase.…”

Section: Adrb3 Plays An Important Anti-obesity Role Because Its Agonistsmentioning

confidence: 52%

“…Yamasaki et al (2013) reported that C57/BL mice fed high-fat diet with a wasabi leaf hot-water extract (without specific pungent components such as allyl isothiocyanate) showed anti-obesity effects. Furthermore, polyphenols are known for their anti-obesity effects (Kamiya et al, 2012;Nishi et al, 2007). Misawa et al (2015) reported that polyphenols contained in wasabi leaves Table 5.…”

Section: Resultsmentioning

confidence: 99%

“…It is a risk factor for lifestyle-related diseases such as hypertension, hyperlipidemia, and diabetes. Thus, the weight loss properties of certain foods, including the inhibition of fat absorption from the intestine and improvement of lipid metabolism, have been studied in obesity prevention (Kamiya et al, 2012;Maeda et al, 2009;Nishi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Anti-obesity Effects of Wasabi Leaf Extract on Rats Fed a High-fat Diet are Related to Upregulation of mRNA Expression of β3-adrenergic Receptors in Interscapular Brown Adipose Tissue

Yamada‐Kato¹,

Momoi

Okunishi³

et al. 2016

FSTR

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Wasabi is a plant of Japanese origin belonging to the Brassicaceae family. Although the wasabi rhizome is a popular condiment in Japan, the leaf is typically discarded. For utilization of the wasabi leaf, we investigated its antiobesity effect on Wistar rats fed a high-fat diet containing wasabi leaf extract (WLE) prepared with 50% ethanol.At the experimental endpoint, WLE had significantly decreased the body weight of rats and upregulated the mRNA expression of the β3-adrenergic receptor (Adrb3) in the interscapular brown adipose tissue (BAT). WLE may have promoted lipid absorption from the dietary fat, as the fecal lipid content was considerably lower in the WLE group. These results suggest that WLE suppressed obesity in rats fed a high-fat diet, which was related to the upregulation of Adrb3 mRNA expression in the interscapular BAT without increased fecal lipid excretion. Thus, wasabi leaves may be used as a functional food material for the suppression of obesity.

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Section: Adrb3 Plays An Important Anti-obesity Role Because Its Agonistsmentioning

confidence: 52%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-obesity Effects of Wasabi Leaf Extract on Rats Fed a High-fat Diet are Related to Upregulation of mRNA Expression of β3-adrenergic Receptors in Interscapular Brown Adipose Tissue

Yamada‐Kato¹,

Momoi

Okunishi³

et al. 2016

FSTR

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“…We previously reported that PFE exerts anti-obesity effects in humans and mice fed high-fat diets [2][3][4]. In addition, we also reported that the isoflavone-rich fraction of dietary PFE exhibits anti-obesity effect in mice fed high-fat diets [5].…”

Section: Introductionmentioning

confidence: 99%

“…The following isoflavones are found in the Kudzu flower but not in soy: 6-hydroxygenistein 6,7-di-O-glucoside (6HGDG), tectorigenin 7-O-xylosylglucoside (TGXG), tectoridin, genistin, glycitin, tectorigenin, genistein, and glycitein [5]. Among these isoflavones, 6HGDG, TGXG, tectoridin and tectorigenin, which comprise nearly 90% of the isoflavones in the Kudzu flower, are present in PFE.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Estrogenic Activity of Pueraria (Kudzu) Flower Extract and Its Major Isoflavones Using ER-Binding and Uterotrophic Bioassays

Kamiya¹,

Takano²,

Yayoi³

et al. 2013

Self Cite

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Pueraria flower extract (PFE) is a hot water extract of the Kudzu flower (Pueraria thomsonii). Tea made from driedKudzu flower is widely used in China, and PFE is utilized as a nutritional supplement in Japan. PFE contains unique isoflavones such as 6-hydroxygenistein 6,7-di-O-glucoside (6HGDG), tectorigenin 7-O-xylosylglucoside (TGXG), and tectoridin. 6HGDG is known to be metabolized into 6-hydroxygenistein, and TGXG and tectoridin are known to be metabolized into tectorigenin in the digestive tract. Isoflavones typically mimic the effects β-estradiol has on estrogen receptors (ERs) and may influence the female genital system in the case of excessive intake. As a result, the upper limit of safe daily consumption of soy isoflavones has been enforced in Japan. In the present study, ER-binding assays were performed using the EnBio estrogen receptor/cofactor assay system to compare the estrogenic activity of 6-hydroxygenistein and tectorigenin to that of the soy isoflavone genistein. In addition, uterotrophic bioassays were performed to investigate the estrogenic effects of PFE in vivo. The ER-binding assays revealed that the ER-binding affinities of 6-hydroxygenistein and tectorigenin were approximately 0.01 -0.04 that of genistein. Soy isoflavone products also induced an increase in uterine wet and blotted weight at doses of 500 mg/kg and 1000 mg/kg, whereas PFE did not cause adverse estrogenic effects, even at a dose of 1000 mg/kg. Based on these results, PFE does not appear to contain compounds with strong estrogenic activity or cause adverse estrogenic effects in vivo. Importantly, the results of this study confirm the safety of PFE as a food supplement.

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Metabolic programming of a beige adipocyte phenotype by genistein

Aziz

Wakeling

Miwa

et al. 2016

Molecular Nutrition Food Res

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ScopePromoting the development of brown or beige adipose tissue may protect against obesity and related metabolic features, and potentially underlies protective effects of genistein in mice.Methods and resultsWe observed that application of genistein to 3T3‐L1 adipocytes changed the lipid distribution from large droplets to a multilocular distribution, reduced mRNAs indicative of white adipocytes (ACC, Fasn, Fabp4, HSL, chemerin, and resistin) and increased mRNAs that are a characteristic feature of brown/beige adipocytes (CD‐137 and UCP1). Transcripts with a role in adipocyte differentiation (Cebpβ, Pgc1α, Sirt1) peaked at different times after application of genistein. These responses were not affected by the estrogen receptor (ER) antagonist fulvestrant, revealing that this action of genistein is not through the classical ER pathway. The Sirt1 inhibitor Ex‐527 curtailed the genistein‐mediated increase in UCP1 and Cebpβ mRNA, revealing a role for Sirt1 in mediating the effect. Baseline oxygen consumption and the proportional contribution of proton leak to maximal respiratory capacity was greater for cells exposed to genistein, demonstrating greater mitochondrial uncoupling.ConclusionsWe conclude that genistein acts directly on adipocytes or on adipocyte progenitor cells to programme the cells metabolically to adopt features of beige adipocytes. Thus, this natural dietary agent may protect against obesity and related metabolic disease.

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The Isoflavone-Rich Fraction of the Crude Extract of the Puerariae Flower Increases Oxygen Consumption and BAT UCP1 Expression in High-Fat Diet-Fed Mice

Cited by 21 publications

References 32 publications

Anti-obesity Effects of Wasabi Leaf Extract on Rats Fed a High-fat Diet are Related to Upregulation of mRNA Expression of β3-adrenergic Receptors in Interscapular Brown Adipose Tissue

Anti-obesity Effects of Wasabi Leaf Extract on Rats Fed a High-fat Diet are Related to Upregulation of mRNA Expression of β3-adrenergic Receptors in Interscapular Brown Adipose Tissue

Evaluation of the Estrogenic Activity of Pueraria (Kudzu) Flower Extract and Its Major Isoflavones Using ER-Binding and Uterotrophic Bioassays

Metabolic programming of a beige adipocyte phenotype by genistein

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