The isolation and identification of the two juvenile hormones from the Cecropia silk moth

Meyer, Andre S.; Hanzmann, Edith; Schneiderman, Howard A.; Gilbert, Lawrence I.; Boyette, Marjorie

doi:10.1016/0003-9861(70)90427-3

Cited by 53 publications

(7 citation statements)

References 51 publications

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“…About 30 years later, Röller et al (1967) were the first to deduce the chemical structure of juvenile hormone (JH I). Subsequently, Meyer et al (1970) confirmed the structure of JH I and identified the structure of a second juvenile hormone (JH II). This and other historical aspects of the discovery and characterization of JHs are elegantly reviewed by Gilbert et al (2000).…”

Section: Introductionmentioning

confidence: 86%

Juvenile hormone (JH) esterase: why are you so JH specific?

Kamita

Hinton

Wheelock

et al. 2003

Insect Biochemistry and Molecular Biology

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Section: Introductionmentioning

confidence: 86%

Juvenile hormone (JH) esterase: why are you so JH specific?

Kamita

Hinton

Wheelock

et al. 2003

Insect Biochemistry and Molecular Biology

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“…The L. longipalpis pheromones (S)-9-methylgermacrene-B as well as 3-methylhimachalene are homosesquiterpenes (C16) that have a branching pattern reminiscent to those observed in the faranal, the trail pheromone of Monomorium pharaoni [75], and the lepidopteran juvenile hormones JH I and JH II [76,77]. Based on the knowledge about the biosynthesis route of these natural homoterpenoid compounds, the construction of L. longipalpis homosesquiterpenes backbone possibly requires molecules derived from homomevalonate, resulting in sesquiterpene backbone molecules with an extra methylene group (CH 3 ), such as the 2E,6E,8S-8-methylfarnesol proposed by Tashiro et al in 2000 [78].…”

Section: Resultsmentioning

confidence: 99%

Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach

González-Caballero¹,

Rodríguez-Vega²,

Dias-Lopes³

et al. 2014

Journal of Proteomics

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In Latin America, Lutzomyia longipalpis is the main vector of the protozoan parasite Leishmania infantum, which is the causal agent of American Visceral Leishmaniasis. This insect uses male-produced pheromones for mate recognition. Elucidation of pheromone biogenesis or its regulation may enable molecular strategies for mating disruption and, consequently, the vector's population management. Motivated by our recent results of the transcriptomic characterization of the L. longipalpis pheromone gland, we performed a proteomic analysis of this tissue combining SDS-PAGE, and mass spectrometry followed by an integrative data analysis. Considering that annotated genome sequences of this sand fly are not available, we designed an alternative workflow searching MS/MS data against two customized databases using three search engines: Mascot, OMSSA and ProLuCID. A total of 542 proteins were confidently characterized, 445 of them using a Uniref100-insect protein database, and 97 using a transcript translated database. In addition, use of PEAKS for de novo peptide sequencing of MS/MS data confirmed ∼90% identifications made with the combination of the three search engines. Our results include the identification of six of the seven enzymes of the mevalonate-pathway, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. Biological significance L. longipalpis is the main vector of the protozoan parasite L. infantum, which is the causal agent of American Visceral Leishmaniasis. One of the control measures of such disease is focused on vector population control. As this insect uses male-produced pheromones for mate recognition, the elucidation of pheromone biogenesis or its regulating process may enable molecular strategies for mating disruption and, consequently, this vector's population management. On this regard, in this manuscript we report expression evidence, at the protein level, of several molecules potentially involved in the pheromone production of L. longipalpis. Our results include the identification of the mevalonate-pathway enzymes, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. In addition, considering that the annotated genome sequences of this sand fly are not yet available, we designed an alternative workflow searching MS/MS data against proteomic and transcript translated customized databases, using three search engines: Mascot, OMSSA, and ProLuCID. In addition, a de novo peptide sequencing software (PEAKS) was used to further analyze the MS/MS data. This approach made it possible to identify and annotate 542 proteins for the pheromone gland of L. longipalpis. Importantly, all annotated protein sequences and raw data are available for the research community in protein repositories that provide free access to the data.

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“…The first examples of homoterpenes, the lepidopteran juvenile hormones, JH I (a bishomofarnesoic ester) and JH II (a homofarnesoic ester) were identified in 1967 and 1968 (Meyer et al, 1970).…”

Section: Discussionmentioning

confidence: 99%

Chemical analysis of oxygenated homosesquiterpenes: a putative sex pheromone from Lutzomyia lichyi (Diptera: Psychodidae)

Hamilton

Brazil

Morgan

et al. 1999

Bull. Entomol. Res.

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Lutzomyia lichyi (Floch & Abbonenc) is a suggested secondary vector ofLeishmania in Colombia. Taxonomically, L. lichyi is very closely related to the L. longipalpis (Lutz & Neiva) species complex. Male members of the complex can be distinguished by their sex pheromones which are produced in papules found grouped together in pale patches on tergites 3 or tergites 3 and 4. Male L. lichyi also have papules, very similar in appearance to those of the L. longipalpis species complex but distributed on tergites 4, 5, 6 and 7. Chemical analysis of male L. lichyi hexane extracts has revealed the presence of two novel oxygenated methylsesquiterpenes. The two major oxygenated terpenes found in the extract of L. lichyi males are tentatively characterized as a primary and tertiary alcohol. The mass spectrum of the proposed primary alcohol is very similar to the published mass spectrum of 3-methyl-␣-himachalene, the sex pheromone produced by the Jacobina chemotype of the L. longipalpis complex. The mass spectrum of the proposed tertiary alcohol also has similarities to the 3-methyl-␣-himachalene sex pheromone.

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The isolation and identification of the two juvenile hormones from the Cecropia silk moth

Cited by 53 publications

References 51 publications

Juvenile hormone (JH) esterase: why are you so JH specific?

Juvenile hormone (JH) esterase: why are you so JH specific?

Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach

Chemical analysis of oxygenated homosesquiterpenes: a putative sex pheromone from Lutzomyia lichyi (Diptera: Psychodidae)

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