The light-harvesting system of a Micromonas species (Prasinophyceae): the combination of three different chlorophyll species in one single chlorophyll–protein complex

Wilhelm, Christian; Lenartz‐Weiler, I.; Wiedemann, I.; Wild, Aloysius

doi:10.2216/i0031-8884-25-3-304.1

Cited by 34 publications

(13 citation statements)

References 9 publications

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“…LB 1965/5, Culture collection, Marine Lab., Plymouth, UK) was grown according to Wilhelm et al (1986). The isolation and purification of the LHC was carried out as described by Herold et al (1991).…”

Section: Methodsmentioning

confidence: 99%

“…In this context the prasinophycean alga Mantoniella squamata is of special interest, because it is regarded as a primitive representative of the chlorophyta ( Van den Hoek et al 1988). Its isolated LHC (Wilhelm et al 1986;Fawley et al 1986) shows an unusual pigment composition, apart from Chl a and Chl b it additionally binds Chl c (Mg 2,4-divinyl pheoporphyrin a5 monomethyl ester) (Jeffrey 1989). The LHC further contains prasinoxanthin as its major carotenoid, which is also involved in energy transfer to Chl.…”

Section: Introductionmentioning

confidence: 99%

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Polypeptide sequence of the chlorophyll a/b/c-binding protein of the prasinophycean alga Mantoniella squamata

Schmitt

Frank²,

James

et al. 1994

Photosynth Res

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The primary structure of the Chla/b/c-binding protein from Mantoniella squamata is determined. This is the first report that protein sequencing reveals one modified amino acid resulting in a LHCP-specific TFA-cleavage site. The comparison of the sequence of Mantoniella with other Chla/b-and Chla/c-binding proteins shows that the modified amino acid is located in a region which is highly conserved in all these proteins. The alignment also reveals that the LHCP of Mantoniella is related to the Chla/b-binding proteins. Finally, possible Chl-binding regions are discussed.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polypeptide sequence of the chlorophyll a/b/c-binding protein of the prasinophycean alga Mantoniella squamata

Schmitt

Frank²,

James

et al. 1994

Photosynth Res

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“…This pigment is also found in several small green flagellates (Micromonadophyceae) (23), although the actual identity of the Chl c-like pigment in these organisms has been questioned (29). In these green algae, Mg 2,4-divinylpheoporphyrin a5 monomethyl ester is present in a light-harvesting complex which also contains Chl a and Chl b (8,30). In Mg-2,4-divinyl-pheoporphyrin a5 monomethyl ester, the acrylic acid group at C7 is replaced by a proprionic acid group.…”

mentioning

confidence: 99%

A New Form of Chlorophyll c Involved in Light-Harvesting

Fawley¹

1989

Plant Physiol.

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“…This latter alga was shown to contain three accessory chlorophylls and prasinoxanthin as dominantxanthophyll [14]. This latter alga was shown to contain three accessory chlorophylls and prasinoxanthin as dominantxanthophyll [14].…”

Section: Introductionmentioning

confidence: 92%

“…They were then homogenized and prepared for SDS PAGE in a procedure given in Wilhelm et al [14]. They were then homogenized and prepared for SDS PAGE in a procedure given in Wilhelm et al [14].…”

Section: Preparation Of Thylakoidsmentioning

confidence: 99%

Energy transfer and pigment composition in three chlorophyll b-containing light-harvesting complexes isolated from Mantoniella squamata (Prasinophyceae), Chlorella fusca (Chlorophyceae) and Sinapis alba

Wilhelm

Lenarz-Weiler

1987

Photosynth Res

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Light-harvesting Chl a/b protein complexes were isolated from the higher plant Sinapis alba, the green alga Chlorella fusca, and the prasinophycean alga Mantoniella squamata by mild gel electrophoresis. The energy transfer from chlorophyll b and the accessory xanthophyll was measured by means of fluoresence spectroscopy at 77 K. The pigment composition of the isolated antenna complexes was determined by high performance liquid chromatography in order to calculate the number of light absorbing molecules per chlorophyll a in the different light-harvesting complexes. These results were complemented by the quantitation of the pigments in total thylakoids as well as in the different electrophoretic fractions. On the basis of these data the in vivo ratios of xanthophylls per chlorophyll a could be estimated. The results show that the light-harvesting complexes from Chlorella and from Sinapis exhibit identical ratios of total xanthophylls per chlorophyll a. By contrast, in the prasinophycean alga Mantoniella, the light-harvesting complex markedly differs from the other chlorophyll b containing proteins. It contains, in addition to neoxanthin and violaxanthin, high amounts of prasinoxanthin and its epoxide, which contribute significantly to light absorption. The concentration of chlorophyll b in the complex is very much higher in the antenna of Mantoniella than in those of Chlorella and Sinapis. Furthermore, it must be emphasized that in addition to chlorophyll b, a third chlorophyll species acts in the energy transfer to chlorophyll a. This chlorophyll c-like pigment is found to be present in a concentration which improves very efficiently the absorption in blue light. In light of these results it can be concluded that the absorption cross section in Mantoniella is higher not only because of an enhanced number of light-harvesting particles in the membrane, but also because of a higher ratio of accessory pigments to chlorophyll a.

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The light-harvesting system of a Micromonas species (Prasinophyceae): the combination of three different chlorophyll species in one single chlorophyll–protein complex

Cited by 34 publications

References 9 publications

Polypeptide sequence of the chlorophyll a/b/c-binding protein of the prasinophycean alga Mantoniella squamata

Polypeptide sequence of the chlorophyll a/b/c-binding protein of the prasinophycean alga Mantoniella squamata

A New Form of Chlorophyll c Involved in Light-Harvesting

Energy transfer and pigment composition in three chlorophyll b-containing light-harvesting complexes isolated from Mantoniella squamata (Prasinophyceae), Chlorella fusca (Chlorophyceae) and Sinapis alba

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