The location of GABA<sub>B</sub> receptor binding sites in mammalian spinal cord

Price, G. W.; Kelly, John T.; Bowery, Norman G.

doi:10.1002/syn.890010605

Cited by 118 publications

(49 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GABA B receptor immunoreactivities are distributed in the laminae I-III in the spinal cord and in DRG neurons (Price et al, 1984;Price et al, 1987;Towers et al, 2000), consistent with data showing high density of binding sites for the GABA B agonist baclofen in these areas (Gehlert et al, 1985). Also, the mRNA of all three GABA B subunits is expressed in both the spinal cord and DRG (Towers et al, 2000).…”

Section: Distribution Of Gaba B Receptors In Pain Pathwayssupporting

confidence: 84%

“…GABA B receptors are present in the primary afferent neurons, spinal cord, and brain (Price et al, 1987;Chu et al, 1990;Towers et al, 2000). GABA B receptor immunoreactivities are distributed in the laminae I-III in the spinal cord and in DRG neurons (Price et al, 1984;Price et al, 1987;Towers et al, 2000), consistent with data showing high density of binding sites for the GABA B agonist baclofen in these areas (Gehlert et al, 1985).…”

Section: Distribution Of Gaba B Receptors In Pain Pathwayssupporting

confidence: 82%

“…The function of GABA B receptors is critically determined by the subunits, particularly the GABA B1a subunit (Towers et al, 2000;Gassmann et al, 2004;Vigot et al, 2006). Primary afferent fiber degeneration by capsaicin administration in neonatal rats decreases GABA B receptor density by 50% (Price et al, 1984;Price et al, 1987), suggesting that at least this proportion of GABA B receptors is present on TRPV1-expressing primary afferent terminals. In the brain, GABA B receptor binding sites are present in the thalamus, amygdala, periaqueductal gray, parabrachial nucleus, and medullary raphe nucleus (Chu et al, 1990).…”

Section: Distribution Of Gaba B Receptors In Pain Pathwaysmentioning

confidence: 99%

See 2 more Smart Citations

Modulation of pain transmission by G-protein-coupled receptors

Pan

Zhou

et al. 2008

Pharmacology & Therapeutics

169

116

View full text Add to dashboard Cite

The heterotrimeric G protein-coupled receptors (GPCRs) represent the largest and most diverse family of cell surface receptors and proteins. GPCRs are widely distributed in the peripheral and central nervous systems and are one of the most important therapeutic targets in pain medicine. GPCRs are present on the plasma membrane of neurons and their terminals along the nociceptive pathways and are closely associated with the modulation of pain transmission. GPCRs that can produce analgesia upon activation include opioid, cannabinoid, α 2 -adrenergic, muscarinic acetylcholine, γ-aminobutyric acid B (GABA B ), group II and III metabotropic glutamate, and somatostatin receptors. Recent studies have led to a better understanding of the role of these GPCRs in the regulation of pain transmission. Here, we review the current knowledge about the cellular and molecular mechanisms that underlie the analgesic actions of GPCR agonists, with a focus on their effects on ion channels expressed on nociceptive sensory neurons and on synaptic transmission at the spinal cord level.

show abstract

Section: Distribution Of Gaba B Receptors In Pain Pathwayssupporting

confidence: 84%

Section: Distribution Of Gaba B Receptors In Pain Pathwayssupporting

confidence: 82%

Section: Distribution Of Gaba B Receptors In Pain Pathwaysmentioning

confidence: 99%

See 1 more Smart Citation

Modulation of pain transmission by G-protein-coupled receptors

Pan

Zhou

et al. 2008

Pharmacology & Therapeutics

169

116

View full text Add to dashboard Cite

show abstract

“…1B). Electrophysiological (Dutar & Nicoll, 1988;Calabresi, Mercuri, DeMurtas & Beradi, 1990) and anatomical (Price, Kelly & Bowery, 1987) evidence indicates that GABAB receptors can be located pre-andÏor postsynaptically. Thus we attempted to determine whether pre-andÏor postsynaptic mechanisms were involved in the baclofen-induced reduction of the visual response in the SC.…”

Section: Resultsmentioning

confidence: 99%

Different roles for GABA_A and GABA_B receptors in visual processing in the rat superior colliculus

Binns

Salt

1997

The Journal of Physiology

View full text Add to dashboard Cite

The superficial grey layer of the superior colliculus (SGS) contains a high proportion of GABAergic inhibitory neurones. We have investigated the role of GABA receptors in synaptic transmission of aspects of visual activity in the SGS that may be driven by inhibitory mechanisms, such as surround inhibition and response habituation. Multi‐barrel glass iontophoretic pipettes were used to record single neuronal activity in the SGS of urethane‐anaesthetized rats. Visual stimulation was provided by the display of moving bars and stationary spots of light on a monitor placed in the receptive field. Both ejection of GABA and the GABAB agonist baclofen reduced responses to moving bars (interstimulus intervals ≥ 8 s). The effects of GABA were reversed by the GABAA antagonist bicuculline, and the effects of baclofen were antagonized by the GABAB antagonist CGP 35348. Surround inhibition was estimated by plotting the response to flashed spots of increasing diameter. In controls, expanding the spot diameter beyond the excitatory receptive field caused a decrease in the response. This inhibitory surround was reversibly reduced by bicuculline, but CGP 35348 had no effect. Response habituation is the progressive reduction in the visual response during repetitive stimulus presentation. In controls, the visual response was reduced to 44 ± 3% of its initial level when a stimulus (moving bar) was presented 5 times with an interstimulus interval of 0.5 s. During CGP 35348 ejection, response habituation was reversibly reduced. Bicuculline had no effect on response habituation. The effects of bicuculline on surround inhibition in the superior colliculus are consistent with similar studies in the lateral geniculate nucleus which indicate that GABAA receptors mediate this effect. The function of GABAB receptors in the visual system is less well researched. The reduction of response habituation with CGP 35348 demonstrates that, at least in the SGS, GABAB receptors have an important role in visual transmission which is distinct from that of GABAA receptors.

show abstract

“…This binding accounted for a high percentage of GABA B sites in the dorsal horn (approximately 40% to 50% [102]), but less for GABA A (approximately 20% to 30% [101]). Dorsal rhizotomy produced a reduction in GABA B -binding sites in the superficial laminae comparable with that produced by neonatal capsaicin treatment, suggesting that these presynaptic GABA B receptors are preferentially localized to capsaicin-sensitive, small diameter myelinated and unmyelinated, typically nociceptive, afferents (103). Even though the GABA B receptor has recently been cloned (104), the precise location of the receptor in DRG neurons has yet to be investigated fully by in situ hybridization or immunohistochemistry.…”

Section: Pain Res Manage Vol 5 No 3 Autumn 2000 189mentioning

confidence: 99%

Presynaptic control of nociceptor signalling: Differential influence of Mu Opioid and GABAergic Systems

Riley

Trafton

Basbaum

2000

Pain Research and Management

View full text Add to dashboard Cite

It is difficult to pick a topic to discuss that adequately honours Ron Melzack's many years of pain research. This is particularly problematic if one is cognizant of the tremendous emphasis that Ron puts on the distinction between pain and nociception. Indeed, the thread that binds so many of Ron's studies is that the complexity of the pain experience will not be understood if one exclusively concentrates on the processing of nociceptive messages at the level of the periphery or spinal cord. Ron always emphasized the importance of affective and cognitive components of the pain experience. These elements of the pain experience, so beautifully and memorably discussed in Melzack and Casey's (1) theoretical review, must always be included when this topic is addressed. Dr Allan Basbaum was a student of Ron's in an undergradu- The relative contribution of pre-and postsynaptic controls to the flow of nociceptive information at the level of the spinal cord has been one of Ron Melzack's longstanding interests and a key issue in the formulation of the gate control theory. The authors review their own studies, in which they monitored internalization of the neurokinin-1 receptor to examine specifically the action of two classically inhibitory systems -mu opioid and gamma-amino butyric acid (GABA) -on noxious stimulus-evoked tachykinin signalling in the rat spinal cord. Evidence that opioids and GABAergic controls operate differently on the central consequences of any noxious stimulus-induced substance P release is provided. Whereas at least 80% of the tachykinin signalling remained intact after even the highest concentration of spinal morphine or D-Ala2, NMe-phe4, Glyol5-enkephalin administration, spinal administration of the GABA B receptor agonist baclofen had a dramatic inhibitory effect. These findings are discussed in light of the disappointing clinical utility of baclofen and neurokinin-1 receptor antagonists to combat pain. Key Words: Gamma-amino butyric acid; Mu opioid; Neurokinin 1 receptors; Receptor internalization; Spinal cord; TachykininsCommande présynaptique de la transmission des signaux par les nocicepteurs : mécanisme d'action des opioïdes de type mu et du GABA Le rôle relatif des commandes pré-et postsynaptiques dans la transmission des signaux nociceptifs dans la moelle épinière, en plus de susciter l'intérêt soutenu de Ron Melzack, a constitué un pilier de la théorie du ‹‹ portillon ››. Les auteurs ont passé en revue leurs propres études dans lesquelles ils se sont penchés sur l'endocytose des récep-teurs de la neurokinine 1 afin d'étudier le mécanisme d'action de deux inhibiteurs classiques, les opioïdes de type mu et l'acide gammaaminobutyrique (GABA), sur la transmission de la tachykinine déclen-chée par un stimulus nocif dans la moelle épinière du rat. Ils ont recueilli des preuves selon lesquelles les opioïdes et le GABA agissent différemment sur les effets centraux de la libération de la substance P, provoquée par un stimulus nocif. Tandis qu'au moins 80 % de la transmission des signaux par la tac...

show abstract

The location of GABA_B receptor binding sites in mammalian spinal cord

Cited by 118 publications

References 46 publications

Modulation of pain transmission by G-protein-coupled receptors

Modulation of pain transmission by G-protein-coupled receptors

Different roles for GABA_A and GABA_B receptors in visual processing in the rat superior colliculus

Presynaptic control of nociceptor signalling: Differential influence of Mu Opioid and GABAergic Systems

Contact Info

Product

Resources

About

The location of GABAB receptor binding sites in mammalian spinal cord

Cited by 118 publications

References 46 publications

Modulation of pain transmission by G-protein-coupled receptors

Modulation of pain transmission by G-protein-coupled receptors

Different roles for GABAA and GABAB receptors in visual processing in the rat superior colliculus

Presynaptic control of nociceptor signalling: Differential influence of Mu Opioid and GABAergic Systems

Contact Info

Product

Resources

About

The location of GABA_B receptor binding sites in mammalian spinal cord

Different roles for GABA_A and GABA_B receptors in visual processing in the rat superior colliculus