The magic-bead concept: an integrated approach to nitrogen removal with co-immobilized micro-organisms

Santos, Vítor A. P. Martins dos; Bruijnse, M.; Tramper, J.; Wijffels, R.H.

doi:10.1007/bf00578454

Cited by 44 publications

(27 citation statements)

References 22 publications

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“…The phenomena can be explained by the diffusional limitation of oxygen into the microbial floc and biofilm. To enhance simultaneous nitrification and denitrification, the beads co-immobilizing both nitrifying and denitrifying bacteria were used under aerobic conditions [3,4]. However the performance of co-immobilization system can be affected by intraparticle distribution of bacteria depending on several conditions, i.e., substrate and oxygen transfer into carrier.…”

Section: Introductionmentioning

confidence: 99%

Improved simultaneous nitrification and denitrification in a single reactor by using two different immobilization carriers with specific oxygen transfer characteristics

Nakano

Iwasawa

Ito

et al. 2004

Bioprocess and Biosystems Engineering

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To maximize nitrogen utilization rates during nitrification and denitrification in a simultaneous reaction for direct nitrogen removal from ammonia-nitrogen in a single reactor, two different carriers were applied that immobilized nitrifiers and denitrifiers separately. With the optimized DO concentration and mixing ratio of immobilization carriers, ammonium-nitrogen was successfully removed as designed until the middle phase of treatment where nitrogen removal rate was higher than 83% of the theoretical value, although an imbalance between nitrification and denitrification occurred at a later phase of treatment where residual nitrate-nitrogen concentration was less than 2 mg/l. The new approach using two different carriers to immobilize nitrifiers and denitrifiers separately was proved useful for controlling both nitrification and denitrification rates, enabling the utilization of maximum treatment ability of both nitrifiers and denitrifiers in a single reactor for direct nitrogen removal from ammonium-nitrogen.

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Section: Introductionmentioning

confidence: 99%

Improved simultaneous nitrification and denitrification in a single reactor by using two different immobilization carriers with specific oxygen transfer characteristics

Nakano

Iwasawa

Ito

et al. 2004

Bioprocess and Biosystems Engineering

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“…SND has allowed for ammonia to transform into nitrogen gas in a single aerobic basin due to layers within the biofilms (Zhang et al, 2007). Especially in thick biofilms like those at The Colony WWTP, oxygen is absent from the inner layers of the biofilm, thus creating an environment for heterotrophic denitrifiers to grow next to the outer layer of autotrophic nitrifiers (dos Santos et al, 1996).…”

Section: Biowin Resultsmentioning

confidence: 99%

“…SND occurs when both aerobic and anoxic layers exist in aerobic biofilm systems, thus allowing the transformation of ammonia to nitrogen gas (Zhang et al, 2007). Although integrated fixed-film activated sludge systems are aerobic in nature, it is possible that oxygen may be absent in portions of the biofilm-especially with thick biofilms-thus creating an anoxic environment where heterotrophic denitrifiers may grow (dos Santos et al, 1996). Many conditions must be met, however, for this to take place.…”

Section: Introductionmentioning

confidence: 99%

Nitrification and Denitrification in an Aerobic Integrated Fixed-Film Activated Sludge System

Points¹,

Downing²,

Yu³

2010

proc water environ fed

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The integrated fixed-film activated sludge process is an advantageous alternative for an effective nutrient removal process without increasing reactor or clarifier basin size. Attached biofilms in the IFAS system allow for anoxic layers to be formed in aerobic environments, leading to simultaneous nitrification and denitrification. This paper discusses simultaneous nitrification and denitrification studied at a full-scale IFAS WWTP. A computer process model was developed using BioWin software. Calibrated to the fullscale, denitrification rates determined by the model were compared to experimental batch studies from the full-scale biofilm samples. Dissolved oxygen concentrations increased throughout the full-scale basin from 1.0 mg/L through saturation. However, denitrification was observed and verified with the process model. Modeling results and field sampling observations indicate denitrification rates increase throughout the length of the aerobic basin. This paper compares full-scale denitrification rates (determined experimentally) with those produced by a process model. Conclusions are provided to explain denitrification in an aerobic IFAS basin.

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“…For instance, water denitrification was successfully achieved by co-immobilizing aerobic Nitrosomas europaea for the oxidation of nitrate to nitrite which colonized the surface of alginate beads, whereas an anaerobic denitrifying Pseudomonas sp. colonized the core of the oxygen deprived beads, allowing to reduce nitrite to N 2 (concept of the ''magic bead'', Santos et al 1996). Likewise simultaneous fermentation of a mixture of glucose and xylose was shown to be feasible thanks to the co-immobilization of Saccharomyces cerevisiae and Candida shehatae (Lebeau et al 1997), although these two yeasts did not require the same oxygen level and that xylose was not consumed as long as glucose was not exhausted with a free cell co-culture.…”

Section: Resultsmentioning

confidence: 99%

Mineralization of diuron [3-(3,4-dichlorophenyl)-1, 1-dimethylurea] by co-immobilized Arthrobacter sp. and Delftia acidovorans

et al. 2007

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Mineralization of diuron has not been previously demonstrated despite the availability of some bacteria to degrade diuron into 3,4-dichloroaniline (3,4-DCA) and others that can mineralize 3,4-DCA. A bacterial co-culture of Arthrobacter sp. N4 and Delftia acidovorans W34, which respectively degraded diuron (20 mg l(-1)) to 3,4-DCA and mineralized 3,4-DCA, were able to mineralize diuron. Total diuron mineralization (20 mg l(-1)) was achieved with free cells in co-culture. When the bacteria were immobilized (either one bacteria or both), the degradation rate was higher. Best results were obtained with free Arthrobacter sp. N4 cells co-cultivated with immobilized cells of D. acidovorans W34 (mineralization of diuron in 96 h, i.e., 0.21 mg l(-1 )h(-1) vs. 0.06 mg l(-1 )h(-1) with free cells in co-culture).

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The magic-bead concept: an integrated approach to nitrogen removal with co-immobilized micro-organisms

Cited by 44 publications

References 22 publications

Improved simultaneous nitrification and denitrification in a single reactor by using two different immobilization carriers with specific oxygen transfer characteristics

Improved simultaneous nitrification and denitrification in a single reactor by using two different immobilization carriers with specific oxygen transfer characteristics

Nitrification and Denitrification in an Aerobic Integrated Fixed-Film Activated Sludge System

Mineralization of diuron [3-(3,4-dichlorophenyl)-1, 1-dimethylurea] by co-immobilized Arthrobacter sp. and Delftia acidovorans

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