The maturity in fetal pigs using a multi-fluid metabolomic approach

Lefort, Gaëlle; Servien, Rémi; Quesnel, Hélène; Billon, Yvon; Canario, Laurianne; Iannuccelli, Nathalie; Canlet, Cécile; Paris, Alain; Vialaneix, Nathalie; Liaubet, Laurence

doi:10.1101/2020.03.13.990564

Cited by 4 publications

(3 citation statements)

References 67 publications

(98 reference statements)

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“…This stage includes the Fourier transform stage, phase and baseline correction 61 , and a TSP referencing a 0.0 chemical shift as a reference standard (Supplementary Figure 5). Data analysis was performed using the ASICS package 62 , a package in the R programming language environment that can perform metabolomics analysis on 1 H NMR spectra to identify and quantify metabolites as referred to previous research 63,65 . This package could automatically identify and quantify metabolites in the NMR spectrum using a unique peak pattern (fingerprint) 59,62 .…”

Section: Metabolite Extractionmentioning

confidence: 99%

1H NMR metabolomics analysis of oil palm stem tissue infected by Ganoderma boninense based on field severity Indices

Pancoro

Karima

Apriyanto³

et al. 2022

Sci Rep

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Basal stem rot disease (BSR) caused by G. boninense affects most oil palm plants in Southeast Asia. This disease can be fatal to palm oil production. BSR shows no signs on the tree in the early stages of infection. Therefore, it is essential to find an approach that can detect BSR disease in oil palm, especially at any level of disease severity in the field. This study aims to identify biomarkers of BSR disease in oil palm stem tissue based on various disease severity indices in the field using 1H NMR-based metabolomics analysis. The crude extract of oil palm stem tissue with four disease severity indices was analyzed by 1H NMR metabolomics. Approximately 90 metabolites from oil palm stem tissue were identified.Twenty of these were identified as metabolites that significantly differentiated the four disease severity indices. These metabolites include the organic acid group, the carbohydrate group, the organoheterocyclic compound group, and the benzoid group. In addition, different tentative biomarkers for different disease severity indices were also identified. These tentative biomarkers consist of groups of organic acids, carbohydrates, organoheterocyclic compounds, nitrogenous organic compounds, and benzene. There are five pathways in oil palm that are potentially affected by BSR disease.

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Section: Metabolite Extractionmentioning

confidence: 99%

1H NMR metabolomics analysis of oil palm stem tissue infected by Ganoderma boninense based on field severity Indices

Pancoro

Karima

Apriyanto³

et al. 2022

Sci Rep

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“…Expression profiling of fetal liver of pig differing in utilization and partitioning of energy will point to metabolic pathways, which affect these physiological properties. In addition, the study of the metabolome during prenatal development is a promising way to identify and characterize parameters of piglet maturity at birth [28]. Ultrastructural studies revealed that the fetal pig liver exhibits specific hepatic metabolic competence and hepatopoietic activity at 40–80 dpc, followed by mainly glycogen accumulation [25].…”

Section: Discussionmentioning

confidence: 99%

Prenatal transcript levels and metabolomics analyses reveal metabolic changes associated with intrauterine growth restriction and sex

et al. 2022

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The metabolic changes associated with intrauterine growth restriction (IUGR) particularly affect the liver, which is a central metabolic organ and contributes significantly to the provision of energy and specific nutrients and metabolites. Therefore, our aim was to decipher and elucidate the molecular pathways of developmental processes mediated by miRNAs and mRNAs, as well as the metabolome in fetal liver tissue in IUGR compared to appropriate for gestational age groups (AGA). Discordant siblings representing the extremes in fetal weight at day 63 post conception (dpc) were selected from F2 fetuses of a cross of German Landrace and Pietrain. Most of the changes in the liver of IUGR at midgestation involved various lipid metabolic pathways, both on transcript and metabolite levels, especially in the category of sphingolipids and phospholipids. Differentially expressed miRNAs, such as miR-34a, and their differentially expressed mRNA targets were identified. Sex-specific phenomena were observed at both the transcript and metabolite levels, particularly in male. This suggests that sex-specific adaptations in the metabolic system occur in the liver during midgestation (63 dpc). Our multi-omics network analysis reveals interactions and changes in the metabolic system associated with IUGR and identified an important biosignature that differs between IUGR and AGA piglets.

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“…This is not compatible with e cient and sustainable production. Last, several studies have showed that piglet maturity, de ned as the complete development allowing survival at birth, is likely to be an important determinant of subsequent survival [8][9][10][11]. Accordingly, piglet maturation and maximum fetal and placental growth, which occur towards the end of gestation, result in a nutritional burden of pregnancy for the mother.…”

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confidence: 99%

Sex and fetal genome influence gene expression in pig endometrium at the end of gestation

Bonnet,

Bluy,

Gress

et al. 2023

Preprint

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Background A fine balance of feto-maternal resource allocation is required to support pregnancy, which depends on interactions between maternal and fetal genetic potential, maternal nutrition and environment, endometrial and placental functions. In particular, some imprinted genes have a role in regulating the exchange of nutrients between the mother and the fetus. Results In this study, we investigated the influence of the fetal genome and sex on the expression of 42 genes, including imprinted genes, at the maternal interface (endometrium) during late gestation. Pure and reciprocal crossed fetuses were produced using two extreme breeds for fetal maturity and piglet survival: Large White (LW) and Meishan (MS). Hence, in the same uterus, endometrial samples were associated with its purebred or crossbred fetuses. We first described a change in gene expression in the endometrium during late gestation (14 differentially expressed genes (DEGs)) and between the two extreme breeds (9 DEGs). The change in expression of 11 genes in LW during late gestation compared to two genes in MS suggests a delay in endometrial processes in LW. The analyses highlighted breed differences in the regulation of endometrial angiogenesis, in nutrient transport and energy metabolism. Correlation networks linked endometrial gene expression with fetal biometrics at D90 and placental measures at D110. We pointed out for the first time the impact of fetal sex and genome on endometrial expression at 90 days of gestation, emphasing AMPD3, CITED1 and H19 genes. We have demonstrated that fetal sex affects the expression of five imprinted genes in LW endometrium. Fetal genome influenced the expression of four genes in LW endometrium but not in MS endometrium. Intriguingly, both fetal sex and fetal genome interact to influence endometrial gene expression. Conclusions These data give new evidence for sexual dimorphism in the pregnant endometrium and provide evidence for the contribution of the fetal genome to feto-maternal interactions at the end of gestation. They suggest that the paternal genome may contribute significantly to piglet survival, especially in crossbreeding production systems. They once again demonstrated the plasticity of the endometrium and the importance of deciphering the dialogue between the placenta and the endometrium.

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The maturity in fetal pigs using a multi-fluid metabolomic approach

Cited by 4 publications

References 67 publications

1H NMR metabolomics analysis of oil palm stem tissue infected by Ganoderma boninense based on field severity Indices

1H NMR metabolomics analysis of oil palm stem tissue infected by Ganoderma boninense based on field severity Indices

Prenatal transcript levels and metabolomics analyses reveal metabolic changes associated with intrauterine growth restriction and sex

Sex and fetal genome influence gene expression in pig endometrium at the end of gestation

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