The measurement of blood flow in mouse femur and its correlation with239Pu deposition

Abstract

The concentration of 18F was measured in three parts of the femur and in the blood of 36 mice killed at intervals of from 1 min to 2 h after intravenous injection. Blood supply to the bone sections, calculated by dividing the increase in 18F concentration in bone by the integral of 18F concentration in blood between selected time intervals, was shown to be unaffected by the return of 18F from bone to blood at times earlier than 90 min following injection. The supply of blood per unit mass of bone was greatest in distal end of the femur, less in proximal end and least in shaft. In whole femur average blood flow was 230 mm3 per gram fresh bone per min. The deposition of 239Pu in three parts of the mouse femur was shown to be linearly related to blood flow and the nature of the relationship is discussed.

Pattern of Uptake of Americium-241 by the Rat Skeleton and its Subsequent Redistribution and Retention: Implications for Human Dosimetry and Toxicology

“…Lower concentrations were found in those bones at the extremities of the skeleton including the head, tail, and paws. It is possible that this result reflects either differences in blood-flow to different anatomical regions of the skeleton (Humphreys et al, 1977); or in the case of the head bones a higher than normal ash weight due to the presence of teeth.…”

Plutonium-239 Deposition in the Skeleton of the Mouse

“…It is, therefore, very suitable for studying whether 239 Pu is distributed differently in cortical and trabecular bone . Furthermore, deposition studies in the femur complement earlier studies on blood flow to different parts of this bone (Humphreys, Fisher and Thorne 1977) .…”

“…It seems, then, reasonable to assume that 239 Pu deposition in bone is influenced more by extrinsic features such as blood supply than by the particular nature of the local bone surface . The importance of blood supply in the deposition of 239 Pu has been shown by Humphreys et al (1977), who found a relationship between 239 PU concentration in, and total blood flow to, various regions of the mouse femur . Preliminary results of more detailed studies indicate that in the femur there is also a correlation between 239 Pu deposition per unit area of endosteum or periosteum and blood flow (Green, Howells and Thorne, unpublished) .…”

Uptake and loss of plutonium from osteoclasts and macrophages in the mandibular condyle of the rat

“…Such surfaces were labeled more heavily in those regions of the spongiosa closest to the zone of erosion of the mandibular condylar cartilage. It is speculated that this pattern is related to the closeness of these surfaces to the capillary loops which form the blood supply to this zone [16]. In c o m m o n with the results of studies on the deposition and redistribution patterns of plutonium in the rat rib [7], the distribution of the plutonium fl -tracks showed that little diffusion of plutonium into the calcified bone occurs.…”