The mechanism of white cell reduction by synthetic fiber cell filters

Callaerts, A.; Gielis, M. L.; Sprengers, E.D.; Muylle, L.

doi:10.1046/j.1537-2995.1993.33293158045.x

Cited by 24 publications

(18 citation statements)

References 6 publications

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“…In contrast, with the previous revision C WBC filtration is only performed during the last return pass, and filtration speed depends on gravity as the collection bag is hanging above the filter and the storage bags (flow rates of 80-100 ml/min). Our results are in line with recent studies [21,26] finding much better results of WBC reduction by positioning the storage bag slightly below the level of the filter resulting in flow rates of 15-25 ml/min. Current good manufacturing practice for blood and blood components require process validation and extensive quality control to ensure that the WBC reduction equipment and technology have been applied correctly to meet the WBC and platelet criteria for the final component.…”

Section: Laboratory Analysissupporting

confidence: 93%

“…Furthermore, 90% of the PCs obtained with the new software contained even < 1 × 10 5 WBCs. The reason for this difference in the amount of residual WBCs is related to different filtration flow rates because reduced filtration flow rates have been found to improve WBC removal [21][22][23]27]. With the new revision C2, the leukocyte filter is positioned slightly below the collection bag and the storage bags, resulting in a reduced filtration rate of 8-10 ml/min.…”

Section: Laboratory Analysismentioning

confidence: 99%

“…The WBC filtration performance is influenced by several variables, e.g. the flow rate of the filtration process [21][22][23]. With low filtration flow rates, there is more time for interaction of the WBCs with the filter material, thereby improving WBC retention [21].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of Platelet Concentrates Obtained with Two Different Plateletpheresis Programs of MCS<sup>®</sup>+

Stiegler¹,

Leitner²,

Panzer³

et al. 2002

Transfus Med Hemother

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Background: The new software, revision C2 of the MCS+ (Haemonetics) with continuous filtration provides reduced leukocyte filtration rates of 8–10 ml/min as the collection and storage bags hang in parallel and both are positioned slightly above the level of the leukocyte filter. Filtration is performed during the entire collection procedure. Prolonged active line sensor control enhances the surge mechanism and thus collection efficiency. Return flow rates are adapted to donors’ body weight and observed by the newly introduced citrate re-infusion control. Material and Methods: With regard to these possible advantages, we evaluated in a prospective, randomized trial plateletpheresis with revision C2 in terms of leukocyte contamination, platelet collection efficiency, and donors’ safety. Results were compared with data obtained from platelet collection using the old software, revision C. Cell counts were measured electronically, residual white blood cells were determined by flow cytometry, and pH was measured with a pH meter. Results: The platelet yield (p < 0.05) and the platelet collection efficiency (p < 0.05) are significantly higher with revision C2 (yield: median 4.11 × 10¹¹, range 3.31–6.09 × 10¹¹; collection efficiency: median 68%, range 62–82%) as compared to revision C (yield: median 3.80 × 10¹¹, range 2.76–5.05 × 10¹¹; collection efficiency: median 59%, range 49–69%). Continuously filtered platelet concentrates contained 75% less leukocytes (median 0.27 × 10⁵, range 0.2–1.3 × 10⁵; lower detection limit 0.2 × 10⁵) than platelet concentrates derived with revision C (median 1.1 × 10⁵, range 0.2–56.7 × 10⁵; lower detection limit 0.2 × 10⁵) (p < 0.05). With the new software, citrate reactions were observed in 6/26 donors compared to 10/29 with the old software. Conclusions: Our data indicate that plateletpheresis with revision C2 is a safe procedure and provides a ready-to-use, high-quality platelet product with low leukocyte contamination.

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Section: Laboratory Analysissupporting

confidence: 93%

Section: Laboratory Analysismentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Platelet Concentrates Obtained with Two Different Plateletpheresis Programs of MCS<sup>®</sup>+

Stiegler¹,

Leitner²,

Panzer³

et al. 2002

Transfus Med Hemother

View full text Add to dashboard Cite

show abstract

“…The WBC filtration performance is influenced by several variables, i.e., the flow rate of the filtration process [22][23][24]. With low filtration flow rates, there is more time for interaction of the WBCs with the filter material, thereby possibly improving WBC retention [22,24].…”

Section: Introductionmentioning

confidence: 99%

“…With low filtration flow rates, there is more time for interaction of the WBCs with the filter material, thereby possibly improving WBC retention [22,24]. The flow rate is affected by the position of the filter, collection bag, and storage bags with respect to each other.…”

Section: Introductionmentioning

confidence: 99%

Fuji surge technique and continuous in‐line filtration to improve the quality of single donor platelet concentrates

Moog¹

2002

J of Clinical Apheresis

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White blood cell (WBC)-reduced single donor platelet concentrates (SDPs) can be collected by most cell separators. WBC reduction can be achieved directly during plateletpheresis or by filtration. Continuous filtration with low filtration rates provides SDPs of good purity. To compensate the platelet (PLT) loss due to filtration, the PLT yield in the unfiltered primary product should be optimal. Fifty donors underwent plateletpheresis with the MCS+ blood cell separator (Haemonetics) with the new Fuji surge technique and continuous WBC filtration. Twelve SDPs were analysed for PLT yield, red blood cells (RBC), WBC, and pH after collection (Day 0) and at the end of storage (Day 5). Thereafter, further 38 SDPs were measured for PLT and WBC content in routine production at Day 0. PLT were determined electronically, RBC and WBC were counted manually (Neubauer and Nageotte chamber, respectively). For pH measurement, a pH-meter was used. Mean blood volume processed was 2621 +/- 112 ml in a donation time of 76 +/- 10 min. An average PLT yield of 3.45 +/- 0.88 x 10(11) was collected in a product volume of 325 +/- 77 ml. The collection efficiency was 60.0 +/- 5.5%. WBC contamination of all units tested was 0.046 +/- 0.059 x 10(6) and the RBC content of the SDPs analysed at Day 0 was 0.014 +/- 0.003 x 10(9). The pH was well maintained over the storage period of 5 days. The data indicate that Fuji surge technique and continuous in-line leukocyte filtration allow for the collection of SDPs with high platelet yield and low leukocyte contamination, meeting the Council of Europe quality standards.

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Evaluation of polyacrylonitrile electrospun nano‐fibrous mats as leukocyte removal filter media

et al. 2017

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Removal of leukocytes from blood products is the most effective means for elimination of undesirable side effects and prevention of possible reactions in recipients. Micro-fibrous mats are currently used for removal of leukocytes from blood. In this study, samples of electrospun nano-fibrous mats were produced. The performance of the produced electrospun nano-fibrous mats as means of leukocytes removal from fresh whole blood was both evaluated and compared with that of commercially available micro-fibrous mats. In order to produce the samples, polyacrylonitrile (PAN) nano-fibrous mats were made under different electrospinning conditions. Mean fiber diameter, pore characterization and surface roughness of the PAN nano-fibrous mats were determined using image processing technique. In order to evaluate the surface tension of the fabricated mats, water contact angle was measured. The leukocyte removal performance, erythrocytes recovery percent and hemolysis rate of the nano-fibrous mats were compared. The effectiveness of nano-fibrous mats in removing leukocyte was established using both scanning electron microscope and optical microscope. Results showed that for given weight, the fabricated nano-fibrous mats were not only more efficient but also more cost-effective than their commercial counterparts. Results confirmed that changes in mean fiber diameter, the number of layer and weight of each layer in the absence of any chemical reaction or physical surface modification, the fabricated nano-fibrous mats were able to remove 5-log of leukocytes. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1759-1769, 2018.

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The mechanism of white cell reduction by synthetic fiber cell filters

Cited by 24 publications

References 6 publications

Comparison of Platelet Concentrates Obtained with Two Different Plateletpheresis Programs of MCS<sup>®</sup>+

Comparison of Platelet Concentrates Obtained with Two Different Plateletpheresis Programs of MCS<sup>®</sup>+

Fuji surge technique and continuous in‐line filtration to improve the quality of single donor platelet concentrates

Evaluation of polyacrylonitrile electrospun nano‐fibrous mats as leukocyte removal filter media

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