The mediators of acquired resistance to Listeria monocytogenes are contained within a population of cytotoxic T cells

Chen-Woan, M; McGregor, Douglas D.

doi:10.1016/0008-8749(84)90022-4

Cited by 15 publications

(5 citation statements)

References 20 publications

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“…The pivotal observations of Lane and Unanue (16), and of North (19,20) that cell-mediated immunity to the facultative intracellular bacterial parasite Listeria monocytogenes is mediated by Thy-1+ cells led naturally to the hypothesis that specific resistance to this infection was mediated by T cells. Further data from a number of laboratories (1,3,6,7,9,18,22) have more recently provided the consensus that CD8 T cells, rather than CD4 cells, are the key T-cell subset involved in protective immunity.…”

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confidence: 99%

Listeria monocytogenes infection in beta 2 microglobulin-deficient mice

1993

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t2 microglobulin-deficient mice, in which the 32m gene has been disrupted by homologous recombination, lack functional CD8 T cells and are able to contain but not resolve an intravenous immunizing inoculum of Listena monocytogenes. We present evidence that compensatory immunity in such immunodeficient mice was mediated by a population of -A T-cell receptor-positive cells; in contrast, neither CD4 cells nor natural killer cells appeared to play any part in this process. These data further support the emerging hypothesis that immune cells other than those bearing the co T-cell receptor type can also play an important role in acquired resistance to listeriosis.

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confidence: 99%

Listeria monocytogenes infection in beta 2 microglobulin-deficient mice

1993

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“…The cells of interest were L. monocytogenes-immune TCRI prekiller cells, and their differentiated counterparts. All express the OX8 marker but fail to bind the W3/25 MAb (1,3). The strategy was to negatively select OX8+ T cells from the lymph of L. monocytogenesimmune donors by panning with W3/25 MAb (1,3), an MAb which recognizes OX8-T cells (18).…”

Section: Methodsmentioning

confidence: 99%

“…All express the OX8 marker but fail to bind the W3/25 MAb (1,3). The strategy was to negatively select OX8+ T cells from the lymph of L. monocytogenesimmune donors by panning with W3/25 MAb (1,3), an MAb which recognizes OX8-T cells (18). In some experiments, T-TDL were panned sequentially, first with the W3/25 MAb and subsequently with the OX4 MAb or the DS4.23 MAb.…”

Section: Methodsmentioning

confidence: 99%

“…Protection assay. Resistance to L. monocytogenes was measured in adoptively immunized rats as described elsewhere (1,3). In brief, recipients of T-TDL or subsetenriched fractions thereof were challenged with approximately 3 x 106 L. monocytogenes organisms.…”

Section: Methodsmentioning

confidence: 99%

“…The latter are formed in response to an immunizing L. monocytogenes infection and circulate briefly in the blood, but soon leave that compartment to infiltrate tissues throughout the body (10). There are reasons for thinking that such specifically sensitized, tissue-positioned T cells realize their protective function in foci of infection, where they are restimulated by L. monocytogenes antigens (1,9). The conventional view is that stimulation promotes the release of biologically active molecules (lymphokines) which have secondary effects on other T cells, monocytes, and monocyte-derived macrophages.…”

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Isolation and characterization of protective T cells induced by Listeria monocytogenes

Chen-Woan¹,

McGregor²,

Noonan³

1986

Infect Immun

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Rats convalescing from a recent infection with Listeria monocytogenes generate T cells which can protect recipient rats against a challenge infection with that organism. Using monoclonal antibodies that react with some but not all rat peripheral T cells, the T-cell mediators of acquired resistance to infection (TCRI) were isolated by panning and characterized by using a fluorescence-activated cell sorter. Many L. monocytogenesimmune TCRI were relatively large cells as judged by their light-scattering properties. That finding accords with previously reported cytokinetic data and velocity sedimentation analyses which revealed that the majority of L.monocytogenes-immune TCRI are lymphoblasts. In the current investigation, the surface antigenic profile of these mediator T cells was revealed as W3/25+ OX8+ OX4+ RT6.1-. That phenotype is identical to that of L. monocytogenes-induced prekiller cells which are formed as part of the animal's cell-mediated response to infection. Like prekiller cells and their differentiated counterparts, L. monocytogenes-immune TCRI adhere preferentially to monolayers of syngeneic fibroblasts. The results indicate that L. monocytogenes-immune TCRI belong to a minor subset of peripheral T cells which also contains T cells that have the cytotoxic potential by which L. monocytogenes-induced prekiller cells have been defined.

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In vitro characterization of rat bone marrow-derived dendritic cells and their precursors

Chen-Woan

Delaney

Fournier

et al. 1996

Journal of Leukocyte Biology

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Although the rat is commonly used for basic immunology and transplantation research, phenotypic and functional characterization of rat dendritic cells (DCs) lags behind similar studies in the human and mouse. Therefore, these features were examined using DCs propagated from cultures of rat bone marrow maintained in a medium supplemented with granulocyte-monocyte colony-stimulating factor. Analysis of cytospin preparations of cultured cells showd that DCs arise from OX7+ myelomonocytic precursors. Typical mature rat DCs were morphologically similar to their mouse and human counterparts and expressed major histocompatibility complex (MHC) class II (common part determinant of Ia), OX62 (integrin molecule), OX7 (CD90), ICAM-1 (CD54), and CTLA4 counterreceptor, but were negative for OX8 (CD8), OX19 (CD5), W3/25 (CD4), and ED2, a rat macrophage marker. Functional analysis of OX62+ sorted DCs showed that they could effectively present the soluble antigen ovalbumin to naive T cells in vitro. A combination of anti-MHC class II monoclonal antibody and CTLA4-immunoglobulin inhibited allostimulatory ability more effectively than either reagent alone. Implications for studying the role of DCs in immune responses in the rat are discussed.

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The mediators of acquired resistance to Listeria monocytogenes are contained within a population of cytotoxic T cells

Cited by 15 publications

References 20 publications

Listeria monocytogenes infection in beta 2 microglobulin-deficient mice

Listeria monocytogenes infection in beta 2 microglobulin-deficient mice

Isolation and characterization of protective T cells induced by Listeria monocytogenes

In vitro characterization of rat bone marrow-derived dendritic cells and their precursors

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