The micronucleus assay using peripheral blood reticulocytes from mitomycin C- and cyclophosphamide-treated rats

Hayashi, Makoto; Kodama, Yukio; Awogi, Takumi; Suzuki, Takayoshi; Asita, A.O.; Sofuni, Toshio

doi:10.1016/0165-1218(92)90236-s

Cited by 70 publications

(43 citation statements)

References 8 publications

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“…However, the animals used in this study were submitted to ESTPI, which may have influenced the number of MNNCE found. Since it has been proven that there is an increase in the number of MN by the use of mutagens in rats submitted to total splenectomy 16,34 , we propose that ESTPI would only partially decrease the splenic capture of these cells. Therefore, it is necessary to develop experiments using HBO in rats subjected to total and partial splenectomy as well as the preservation of the entire spleen.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the important role in micronucleated cells splenic capture and since little is known about the impact of HBO on MN frequency on partially splenectomized animals 16 , we evaluate the mutagenic potential of a HBO protocol by means of a micronucleus test performed in the peripheral blood of Rattus norvegicus Wistar rats submitted to ESTPI, according to techniques already described [17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

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Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole

et al. 2015

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PURPOSE:To assess the mutagenic potential of the oxygen inhalation therapy (HBO), by means of the micronucleus test, performed in peripheral blood of rats that underwent subtotal splenectomy with lower pole preservation (ESTPI), after HBO sessions or simulations. METHODS:Eighteen male Wistar rats, were distributed into three groups of six animals: group 1 -submitted to ESTPI and HBO sessions; group 2 -submitted to ESTPI and HBO simulations; group 3 -underwent cyclophosphamide administration. In groups 1 and 2, blood samples from the animals' tails were collected before surgery (T0) and immediately after the 13th HBO session or simulation (T1). In group 3, tail blood samples were collected from animals before (T0) and 24 hours after (T1) cyclophosphamide (CP) delivery.The number of micronucleated normochromatic erythrocytes (MNNCE) was determined by blind counting 2000 normochromatic erythrocytes (NCE) per animal. RESULTS:Micronuclei average after CP delivery in group 3 was higher than before its use, thus confirming the mutagenic activity of this drug (p=0.01). In groups 1 and 2, no significant difference in the average of Micronuclei was observed when comparing it to blood samples before and after the 13th HBO session or simulation. CONCLUSION:The treatment protocol used in this study did not induce Micronucleus formation in animals submitted to ESTPI and HBO treatment or simulation.Key words: Splenectomy. Mutagenicity Tests. Oxygen Inhalation Therapy. Rats. Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole

et al. 2015

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“…Each samples dissolved in DMSO was administered orally at a dose of 2.0 mmol/kg of body weight. After 24 h and 48 h, 5 mL of peripheral blood was collected from the tail vein and applied to acridine orange-coated glass slides for an assay by the method of Hayashi et al (1992).…”

Section: Synthesis Ofmentioning

confidence: 99%

Change in Mutagenic Activity of Genistein after a Nitrite Treatment

Masuda

Shimamura

Kato

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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“…5). [28][29][30][31] It has been shown that bone marrow cells can be replaced by peripheral cells without any problem in sensitivity.…”

Section: The Development and Improvement Of A Peripheral Blood Micronmentioning

confidence: 99%

Short-Term Screening Method for the Prediction of Carcinogenicity of Chemical Substances. Current Status and Problems of an in vivo Rodent Micronucleus Assay.

Sato

Tomita

2001

JOURNAL OF HEALTH SCIENCE

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Various short-term screening methods have been developed to detect mutagenic/carcinogenic substances. They have played important roles not only in screening suspected chemicals but in studying the mechanisms of mutagenesis/carcinogenesis, and have provided useful information for assessing the genetic effects of chemicals on humans. The micronucleus assay is an in vivo mammalian methods, which has been widely used for screening the genotoxic potency of chemical substances. By this assay, the genotoxicity of chemicals, including clastogens and spindle poisons, have been evaluated using immature bone marrow erythrocytes in mice. The use of immature erythrocytes in circulating peripheral blood instead of in bone marrow of mice has been developed in recent years and it has been determined that it gives results as relevant as those of bone marrow cells in mice. Using the peripheral blood of mice has made the micronucleus assay method more practical and useful. Recently, rats have been identified as an acceptable species for this assay, and assays using the peripheral blood of rats are often carried out concomitantly with general toxicity or carcinogenic studies. However, it must be noted that the micronucleus assays have some limitations. The activity of substances which are metabolized rapidly in mice or rats, for example, may hardly be detected by the micronucleus assay; metabolic features of the substances may affect the assay results greatly. In this review, the current status of the micronucleus assay is discussed as a short-term screening method in the context of its usefulness and its limitations.

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The micronucleus assay using peripheral blood reticulocytes from mitomycin C- and cyclophosphamide-treated rats

Cited by 70 publications

References 8 publications

Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole

Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole

Change in Mutagenic Activity of Genistein after a Nitrite Treatment

Short-Term Screening Method for the Prediction of Carcinogenicity of Chemical Substances. Current Status and Problems of an in vivo Rodent Micronucleus Assay.

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