The microRNA regulated SBP-box genes SPL9 and SPL15 control shoot maturation in Arabidopsis

Schwarz, Štefan; Grande, Arne V.; Bujdoso, Nora; Saedler, Heinz; Huijser, Peter

doi:10.1007/s11103-008-9310-z

Cited by 453 publications

(457 citation statements)

References 42 publications

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“…We found that spl9 spl15 double mutants had short plastochrons (Figure 1; see Supplemental Table 1 online), in agreement with a recent independent report (Schwarz et al, 2008). Although the phenotype was milder than that of the miR156 overexpressers, in which expression of eight additional SPL genes is strongly reduced (Schwab et al, 2005), this finding indicated an important redundant effect of SPL9 and SPL15 on plastochron length.…”

Section: Role Of Mir156-targeted Spl Genes In Determining Plastochronsupporting

confidence: 91%

“…One includes microRNA156 (miR156), which targets several SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor genes that are predominantly expressed at the shoot apex (Cardon et al, 1999;Rhoades et al, 2002;Schmid et al, 2003;Schwarz et al, 2008). By comparing endogenous expression domains of SPL genes with the results of misexpression studies, we demonstrate that miR156 limits plastochron length in developing leaf primordia.…”

Section: Introductionmentioning

confidence: 99%

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Dual Effects of miR156-TargetedSPLGenes andCYP78A5/KLUHon Plastochron Length and Organ Size inArabidopsis thaliana

et al. 2008

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Leaves of flowering plants are produced from the shoot apical meristem at regular intervals, with the time that elapses between the formation of two successive leaf primordia defining the plastochron. We have identified two genetic axes affecting plastochron length in Arabidopsis thaliana. One involves microRNA156 (miR156), which targets a series of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes. In situ hybridization studies and misexpression experiments demonstrate that miR156 is a quantitative, rather than spatial, modulator of SPL expression in leaf primordia and that SPL activity nonautonomously inhibits initiation of new leaves at the shoot apical meristem. The second axis is exemplified by a redundantly acting pair of cytochrome P450 genes, CYP78A5/KLUH and CYP78A7, which are likely orthologs of PLASTOCHRON1 of rice (Oryza sativa). Inactivation of CYP78A5, which is expressed at the periphery of the shoot apical meristem, accelerates the leaf initiation rate, whereas cyp78a5 cyp78a7 double mutants often die as embryos with supernumerary cotyledon primordia. The effects of both miR156-targeted SPL genes and CYP78A5 on organ size are correlated with changes in plastochron length, suggesting a potential compensatory mechanism that links the rate at which leaves are produced to final leaf size.

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Section: Role Of Mir156-targeted Spl Genes In Determining Plastochronsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Dual Effects of miR156-TargetedSPLGenes andCYP78A5/KLUHon Plastochron Length and Organ Size inArabidopsis thaliana

et al. 2008

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“…4) (refs 29,31). SPL9, À 10, À 2, À 3, À 11, À 13 and À 15 have been shown to function similarly and redundantly with each other in regulation of the juvenile-to-adult phase transition and flowering time [29][30][31][32][33][34][35][36] . SPLs also feedback-regulate miR156 precursor transcription 29 .…”

Section: Resultsmentioning

confidence: 99%

The effects of carbon dioxide and temperature on microRNA expression in Arabidopsis development

et al. 2013

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Elevated levels of CO 2 and temperature can both affect plant growth and development, but the signalling pathways regulating these processes are still obscure. MicroRNAs function to silence gene expression, and environmental stresses can alter their expressions. Here we identify, using the small RNA-sequencing method, microRNAs that change significantly in expression by either doubling the atmospheric CO 2 concentration or by increasing temperature 3-6°C. Notably, nearly all CO 2 -influenced microRNAs are affected inversely by elevated temperature. Using the RNA-sequencing method, we determine strongly correlated expression changes between miR156/157 and miR172, and their target transcription factors under elevated CO 2 concentration. Similar correlations are also found for microRNAs acting in auxin-signalling, stress responses and potential cell wall carbohydrate synthesis. Our results demonstrate that both CO 2 and temperature alter microRNA expression to affect Arabidopsis growth and development, and miR156/157-and miR172-regulated transcriptional network might underlie the onset of early flowering induced by increasing CO 2 .

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“…AGL24, FPF1, and SPL9 promote flowering (Kania et al, 1997;Melzer et al, 1999;Michaels et al, 2003;Schwarz et al, 2008;Wang et al, 2009), whereas FLC, TEM1, TFL1, MAF2, and MAF3 repress flowering (Michaels and Amasino, 1999;Ratcliffe et al, 2003;Castillejo and Pelaz, 2008).…”

Section: Flowering-time Genesmentioning

confidence: 99%

EMBRYONIC FLOWER1 and ULTRAPETALA1 Act Antagonistically on Arabidopsis Development and Stress Response

Liu

Kim

et al. 2013

Plant Physiology

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Epigenetic regulation of gene expression is of fundamental importance for eukaryotic development. EMBRYONIC FLOWER1 (EMF1) is a plant-specific gene that participates in Polycomb group-mediated transcriptional repression of target genes such as the flower MADS box genes AGAMOUS, APETALA3, and PISTILLATA. Here, we investigated the molecular mechanism underlying the curly leaf and early flowering phenotypes caused by reducing EMF1 activity in the leaf primordia of LFYasEMF1 transgenic plants and propose a combined effect of multiple flower MADS box gene activities on these phenotypes. ULTRAPETALA1 (ULT1) functions as a trithorax group factor that counteracts Polycomb group action in Arabidopsis (Arabidopsis thaliana). Removing ULT1 activity rescues both the abnormal developmental phenotypes and most of the misregulated gene expression of LFYasEMF1 plants. Reducing EMF1 activity increases salt tolerance, an effect that is diminished by introducing the ult1-3 mutation into the LFYasEMF1 background. EMF1 is required for trimethylating lysine-27 on histone 3 (H3K27me3), and ULT1 associates with ARABIDOPSIS TRITHORAX1 (ATX1) for trimethylating lysine-3 on histone 4 (H3K4me3) at flower MADS box gene loci. Reducing EMF1 activity decreases H3K27me3 marks and increases H3K4me3 marks on target gene loci. Removing ULT1 activity has the opposite effect on the two histone marks. Removing both gene activities restores the active and repressive marks to near wild-type levels. Thus, ULT1 acts as an antirepressor that counteracts EMF1 action through modulation of histone marks on target genes. Our analysis indicates that, instead of acting as off and on switches, EMF1 and ULT1 mediate histone mark deposition and modulate transcriptional activities of the target genes.

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The microRNA regulated SBP-box genes SPL9 and SPL15 control shoot maturation in Arabidopsis

Cited by 453 publications

References 42 publications

Dual Effects of miR156-TargetedSPLGenes andCYP78A5/KLUHon Plastochron Length and Organ Size inArabidopsis thaliana

Dual Effects of miR156-TargetedSPLGenes andCYP78A5/KLUHon Plastochron Length and Organ Size inArabidopsis thaliana

The effects of carbon dioxide and temperature on microRNA expression in Arabidopsis development

EMBRYONIC FLOWER1 and ULTRAPETALA1 Act Antagonistically on Arabidopsis Development and Stress Response

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