The molecular organization of the Antennapedia locus of drosophila

Scott, Matthew P.; Weiner, Amy J.; Hazelrigg, Tulle; Polisky, Barry; Pirrotta, Vincenzo; Scalenghe, Franco; Kaufman, Thomas C.

doi:10.1016/0092-8674(83)90109-5

Cited by 329 publications

(179 citation statements)

References 55 publications

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“…Thus, it appears that altering the net positive charge of the BBR is likely to affect the DNA binding function of Barx proteins. 2 Such modulation of DNA binding by an adjacent peptide domain has been observed in other homeodomain proteins. For instance, the POU domain can bind independently to DNA and also change the DNA target specificity of the POU type homeodomain (12).…”

Section: Discussionmentioning

confidence: 92%

The Homeodomain Protein Barx2 Contains Activator and Repressor Domains and Interacts with Members of the CREB Family

Edelman¹,

Meech²,

Jones³

2000

Journal of Biological Chemistry

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Barx1 and Barx2 are homeodomain proteins originally identified using regulatory elements of genes encoding certain cell adhesion molecules (CAMs). In the present study, we characterize regions of Barx2 that bind to regulatory elements of genes encoding three CAMs, L1, neuron-glia CAM (Ng-CAM), and neural CAM (N-CAM), and identify domains of Barx2 that regulate N-CAM transcription. The homeodomain of Barx2 was sufficient for binding to homeodomain binding sites (HBS) from all three CAM genes. The presence of a 17-amino acid Barx basic region resulted in a 2-fold decrease in binding to HBS sequences from the Ng-CAM and L1 genes, whereas it led to a 6.5-fold increase in binding to the HBS from the N-CAM promoter. Thus, the Barx basic region influences the strength and specificity of Barx2 binding to DNA. In co-transfection experiments, Barx2 repressed N-CAM promoter activity. A 24-residue N-terminal region of Barx2 was essential for repression. When this region was absent, Barx2 activated the N-CAM promoter. A 63-residue C-terminal domain was required for this activation. In GST pull-down experiments, Barx2 bound to proteins of the CREB family, CREB1 and ATF2. Overall, these findings provide a framework for understanding developmental and physiological contexts that influence repressor or activator functions of Barx2.

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Section: Discussionmentioning

confidence: 92%

The Homeodomain Protein Barx2 Contains Activator and Repressor Domains and Interacts with Members of the CREB Family

Edelman¹,

Meech²,

Jones³

2000

Journal of Biological Chemistry

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“…Scott et al (52) identified two major Antp poly(A)+ RNA species, 5.0 and 3.5 kb in size, which are present throughout development. The 3.5-kb species predominates during early embryonic stages but is replaced by the 5.0-kb RNA by the end of this period.…”

Section: Resultsmentioning

confidence: 99%

“…This preference is independent of whether the RNA contains exon 1 or exon 3. Small Antp RNAs. Scott et al (52) reported that exon 1 hybridizes to at least one small RNA. We show that this exon may hybridize to several poly(A)+ RNAs about 1 kb long (Fig.…”

Section: Resultsmentioning

confidence: 99%

Multiple transcripts from the Antennapedia gene of Drosophila melanogaster.

Stroeher¹,

Jørgensen²,

Garber³

1986

Mol. Cell. Biol.

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“…The Antp gene is unusually large; its eight exons span over 100 kilobase pairs (kb)of genomic DNA Scott et al 1983;Laughon et al 1986;Schneuwly et al 1986). The open reading flame is contamed in four major transcript types, which are generated by alternative choices at two steps in RNA synthesis: initiation and 3' processing {Fig.…”

mentioning

confidence: 99%

Function and misfunction of the two promoters of the Drosophila Antennapedia gene.

Jørgensen¹,

Garber²

1987

Genes Dev.

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In the Antennapedia (Antp) gene of Drosophila melanogaster, structurally distinct RNAs arise from different transcription initiation sites. When the two sites are separated by a chromosome inversion, transcripts are produced from each fragment of the split Antp locus, and these RNAs initiate at the same nucleotide as in wild-type animals. Thus, the initiation sites are regulated by independent promoters. We show by in situ hybridization that transcripts from each promoter accumulate in spatially distinct patterns in a subset of wildtype imaginal discs. Importantly, these patterns are generally maintained in the inversion mutant. We conclude that the promoters possess independent and dissimilar regulatory elements for spatial activation. Finally, we have looked at transcription in seven different dominant Antp mutants, all of which show a transformation of head tissue to thoracic tissue. In each mutant, the second promoter is improperly activated in the eye-antennal imaginal disc. Because all but one of these mutations have inversion breakpoints distantly upstream of the activated promoter, they probably act via long-range euchromatic position effects. Our studies define how the dual promoters and chromatin structure of the Antp gene contribute to the generation of a complex pattern of transcription.

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The molecular organization of the Antennapedia locus of drosophila

Cited by 329 publications

References 55 publications

The Homeodomain Protein Barx2 Contains Activator and Repressor Domains and Interacts with Members of the CREB Family

The Homeodomain Protein Barx2 Contains Activator and Repressor Domains and Interacts with Members of the CREB Family

Multiple transcripts from the Antennapedia gene of Drosophila melanogaster.

Function and misfunction of the two promoters of the Drosophila Antennapedia gene.

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