The molecular structure of the mammalian genome

Ginatulina, L. K.; Ginatulin, A. A.; Vorontsov, N. N.

doi:10.1007/bf00121823

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…gorbuscha involved sites in the IGS which show some intraspecific variability. A recent analysis of mtDNA of Pacific salmon from the Soviet Union (Ginatulina et al, 1988) confirmed the close relationship between mtDNA of 0. keta and 0 . gorbuscha.…”

Section: Comparison Of Ribosomal Dna Results With Mitochondrial Dna Rmentioning

confidence: 55%

Nuclear DNA and salmonid phylogenetics

Phillips

Pleyte

1991

Journal of Fish Biology

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There are many unresolved problems in salmonid systematics, both at the interspecific and subspecific levels. Some of the major systematic problems in the subfamily Salmoninae are briefly reviewed along with the available molecular methods for their analysis. Nuclear DNA markers available for use in molecular systematics include localized and dispersed highly repetitive DNA sequences, moderately repetitive sequences such as the ribosomal RNA genes (rDNA), and single copy DNA sequences. Both coding and non-coding sequences can be examined in the rDNA and single copy DNA. The rDNA is especially suitable for use in phylogenetic analysis, since different regions evolve at different rates and can be used for comparisons at different taxonomic levels.Comparison of restriction maps of the entire rDNA repeating unit in 17 salmonid species from Hurllo. Sahelinus, Sulmo and Oncorhynchus has shown that the transcribed spacer regions are the most informative for interspecific comparisons and that the intergenic spacer has potential for use in intraspecific comparisons. Our current approach is to amplify selected regions from each of these spacers for analysis by DNA sequencing. DNA sequence analysis of the internal transcribed spacers should be very informative in elucidating interspecific relationships in Sulvelinus and Oncorhynchus. Analysis of a hypervariable region in the intergenic spacer has potential for identification of geographically separated stocks. The relative utility of different types of nuclear DNA sequences for identification of stocks and subspecies is examined.

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Section: Comparison Of Ribosomal Dna Results With Mitochondrial Dna Rmentioning

confidence: 55%

Nuclear DNA and salmonid phylogenetics

Phillips

Pleyte

1991

Journal of Fish Biology

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“…A similar situation was found previously in the comparative analysis of DNA in Cricetus cricetus . In a number of papers the genome size has been compared to the proportion of C-banded heterochromatin and, in some cases, a positive relationship has been noted (Hatch et al, 1976;Deaven et al, 1977;Narayan & Rees, 1976;Mascarello & Mazrimas, 1977;Ginatulina et al, 1980). For example, representatives of the genus Ammospermophilus, which is close to Citellus, have increased amounts of both C-banded sites in their chromosomes and total DNA per cell when compared with ground squirrels from the other genus Ictidomys spilosoma (the diploid size of the genome ofA.…”

Section: Discussionmentioning

confidence: 99%

“…Repeats of mammals account for less than a half of a genome, i.e. 30-50% (Santiago & Rake, 1973;Schmid & Deininger, 1975;Straus, 1976;Pearson et al, 1978;Miroshnichenko et al, 1978;Ginatulina et al, 1980). In mammals 3 repeated sequence components can be identified in kinetic curves of DNA reassociation: one fraction of very fast reassociating sequences, including both the inverted repeats and highly repeated sequences (more than 106 copies per genome) and two fractions with moderate rate of sequence reassociation representUrocitellus; real ground squirrels, Citellus s. str.…”

Section: Introductionmentioning

confidence: 99%

Genome analysis of ground squirrels of the genus Citellus (Rodentia, Sciuridae) I. DNA reassociation kinetics and genome size of eight species

et al. 1982

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Kinetic of reassociation of short DNA fragments were measured in eight ground squirrel species: Citellus undulatus, C. parryL C. relictus, C. dauricus, C. citellus, C. pygmaeus, C. fulvus and C. major. It was shown that 30-50% of their genome were represented by repeated sequences forming three kinetic fractions, i,e., very fast(Cot< 10 3), fast(Cot 10 -3 -3 × I0 -l) and intermediate (Cot 6 × 10 -1 -6 × 101). Based on parameters of DNA reassociation kinetics genome sizes of Citellus were estimated to range from 2.7 pg (C. dauricus) to 3.9 pg (C. pygmaeus and C.fulvus). Variation in genome sizes involves both the repeated and the non-repeated sequence components to approximately equal extents in all the species except C. dauricus. The linear quantitative relation between C-banding heterochromatin and both very fast and fast reassociated DNA fractions was established, but no connection with the intermediate fraction was found. No distinct relation was revealed between parameters of DNA reassociation kinetics and taxonomic status of species within genus or with the chromosome number of the karyotype.

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