The mosquitoes (Diptera: Culicidae) of Tabasco, Mexico

Ortega‐Morales, Aldo I.; Méndez-López, Ramón; Garza-Hernández, Javier A.; González-Álvarez, Vicente Homero; Ruíz-Arrondo, Ignacio; Huerta-Jiménez, Herón; Rodriguez-Martinez, Luis Miguel; Rodríguez‐Pérez, Mario A.

doi:10.1111/jvec.12329

Cited by 18 publications

(17 citation statements)

References 17 publications

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“…albipes and Ps. ferox in the circulation of Venezuelan equine encephalitis virus (VEEV), WNV, and LaCrosse virus in tropical regions has been well-established (62). By contrast to the focused host use of other species, Cx.…”

Section: Discussionmentioning

confidence: 99%

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An Integrated Molecular Approach to Untangling Host–Vector–Pathogen Interactions in Mosquitoes (Diptera: Culicidae) From Sylvan Communities in Mexico

et al. 2021

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There are ~240 species of Culicidae in Mexico, of which some are vectors of arthropod-borne viruses such as Zika virus, dengue virus, chikungunya virus, and West Nile virus. Thus, the identification of mosquito feeding preferences is paramount to understanding of vector–host–pathogen interactions that, in turn, can aid the control of disease outbreaks. Typically, DNA and RNA are extracted separately for animal (insects and blood meal hosts) and viral identification, but this study demonstrates that multiple organisms can be analyzed from a single RNA extract. For the first time, residual DNA present in standard RNA extracts was analyzed by DNA barcoding in concert with Sanger and next-generation sequencing (NGS) to identify both the mosquito species and the source of their meals in blood-fed females caught in seven sylvan communities in Chiapas State, Mexico. While mosquito molecular identification involved standard barcoding methods, the sensitivity of blood meal identification was maximized by employing short primers with NGS. In total, we collected 1,634 specimens belonging to 14 genera, 25 subgenera, and 61 morphospecies of mosquitoes. Of these, four species were new records for Mexico (Aedes guatemala, Ae. insolitus, Limatus asulleptus, Trichoprosopon pallidiventer), and nine were new records for Chiapas State. DNA barcode sequences for >300 bp of the COI gene were obtained from 291 specimens, whereas 130 bp sequences were recovered from another 179 specimens. High intraspecific divergence values (>2%) suggesting cryptic species complexes were observed in nine taxa: Anopheles eiseni (5.39%), An. pseudopunctipennis (2.79%), Ae. podographicus (4.05%), Culex eastor (4.88%), Cx. erraticus (2.28%), Toxorhynchites haemorrhoidalis (4.30%), Tr. pallidiventer (4.95%), Wyeomyia adelpha/Wy. guatemala (7.30%), and Wy. pseudopecten (4.04%). The study increased the number of mosquito species known from 128 species to 138 species for Chiapas State, and 239 for Mexico as a whole. Blood meal analysis showed that Aedes angustivittatus fed on ducks and chicken, whereas Psorophora albipes fed on humans. Culex quinquefasciatus fed on diverse hosts including chicken, human, turkey, and Mexican grackle. No arbovirus RNA was detected by reverse transcriptase–polymerase chain reaction in the surveyed specimens. This study demonstrated, for the first time, that residual DNA present in RNA blood meal extracts can be used to identify host vectors, highlighting the important role of molecular approaches in both vector identification and revealing host–vector–pathogen interactions.

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Section: Discussionmentioning

confidence: 99%

“…ferox) are pathogen vectors in Mexico and other countries in the neotropics. Given the medical importance of the viruses that they transmit(60)(61)(62)(63), the need for regular vector surveillance to aid disease control is essential in Mexico and throughout Central America.…”

mentioning

confidence: 99%

An Integrated Molecular Approach to Untangling Host–Vector–Pathogen Interactions in Mosquitoes (Diptera: Culicidae) From Sylvan Communities in Mexico

et al. 2021

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“…In addition, access to PCBR is challenging, and as a result, it was not possible to obtain specimens from all localities. All 35 species collected in this study were previously recorded in the survey undertaken by Ortega-Morales et al (2019a) in Tabasco State.…”

Section: Discussionmentioning

confidence: 99%

DNA Barcoding of Mosquitoes from the Pantanos de Centla Biosphere Reserve, Southeastern Mexico

Ortega‐Morales¹,

Hernández‐Triana²,

Chan-Chablé³

et al. 2021

Journal of the American Mosquito Control Association

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Accurate identification of mosquito species is essential to support programs that involve the study of distribution and mosquito control. Numerous mosquito species are difficult to identify based only on morphological characteristics, due to the morphological similarities in different life stages and large numbers of some species that are members of morphologically similar species complexes. In the present study, the mosquitoes collected in the Pantanos de Centla Biosphere Reserve, southeastern Mexico, were evaluated using a combination of morphological and molecular approaches (mitochondrial cytochrome c oxidase subunit I [COI] DNA barcode). A total of 1,576 specimens of 10 genera and 35 species, mostly adult stages, were collected. A total of 225 COI DNA barcode sequences were analyzed; most species formed well-supported groups in the neighbor joining, maximum likelihood, and Bayesian inference trees. The intraspecific Kimura 2-parameter (K2P) genetic distance averaged 1.52%. An intraspecific K2P distance of 6.20% was observed in Anopheles crucians s.l., while a deep split was identified in Culex erraticus and Cx. conspirator. This study showed that COI DNA barcodes offer a reliable approach to support mosquito species identification in Mexico.

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“…dyari was described, historical records of these species across different Mexican states are thus doubtful. Mansonia indubitans has been reported in the states of Baja California, Baja California Sur, Campeche, Chiapas, Guerrero, Quintana Roo, Tabasco, Tamaulipas, Veracruz (Díaz-Nájera 1966;Díaz-Nájera and Vargas 1973;García-Aldrette and Pletsch 1976;Ibáñez-Bernal and Martínez-Campos 1994;Ríos-López et al 2008;Deardroff et al 2011;Ibáñez-Bernal et al 2011;Dzul-Manzanilla et al 2013;Ortega-Morales et al 2013, 2019a (Ortega-Morales et al 2019b). Recently, mosquito collection has been conducted in the states of Tlaxcala, Hidalgo, and Mexico City, but no Mansonia species had been found (Muñoz-Cabrera et al 2006, Ortega-Morales et al 2018, Dávalos-Becerril et al 2019.…”

Section: Mansonia Dyari Was Originally Described Bymentioning

confidence: 99%

First Record of Mansonia dyari in the State of Morelos, Mexico, Based on Morphology and COI DNA Barcoding

Hernández-Guevara

Sánchez-Ramos

Chan-Chablé

et al. 2020

Journal of the American Mosquito Control Association

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Collections of mosquitoes were conducted for the surveillance of species of medical importance in the state of Morelos, Mexico, in June 2017. Species collected included Mansonia ( Mansonia) dyari, which was identified using morphological characters and cytochrome c oxidase I DNA barcoding. Although 3 species of genus Mansonia have been previously reported in Mexico, this is the 1st confirmed record of Ma. dyari in Morelos State, where no Mansonia species had been recorded. Historical records of Ma. dyari and Ma. indubitans in Mexico were reviewed. Therefore, this record increases the number of mosquito species occurring in Morelos to 46. The specimens collected in this study were deposited in the Culicidae collection of the Universidad Autónoma Agraria Antonio Narro, Unidad Laguna.

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The mosquitoes (Diptera: Culicidae) of Tabasco, Mexico

Cited by 18 publications

References 17 publications

An Integrated Molecular Approach to Untangling Host–Vector–Pathogen Interactions in Mosquitoes (Diptera: Culicidae) From Sylvan Communities in Mexico

An Integrated Molecular Approach to Untangling Host–Vector–Pathogen Interactions in Mosquitoes (Diptera: Culicidae) From Sylvan Communities in Mexico

DNA Barcoding of Mosquitoes from the Pantanos de Centla Biosphere Reserve, Southeastern Mexico

First Record of Mansonia dyari in the State of Morelos, Mexico, Based on Morphology and COI DNA Barcoding

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