The natural product 4,10-aromadendranediol induces neuritogenesis in neuronal cells in vitro through activation of the ERK pathway

Chang, Su-Youne; Ruan, Wenchen; Xu, Yang; Pang, Jie; Zhang, Luyong; Liao, Hong; Pang, Tao

doi:10.1038/aps.2016.115

Cited by 17 publications

(5 citation statements)

References 33 publications

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“…These ROS are considered to be one of the primary causes for the endothelial cytotoxicity of DL-Hcy. Chang and coauthors showed stimulation of ROS generation by 0.5 and 1.0 mmol/L Hcy in rat vascular smooth muscle cells (VSMC) (27). The same study also demonstrated that CAT activity in VSMC was inhibited by Hcy, which is consistent with the results of our research.…”

Section: Discussionsupporting

confidence: 91%

The Effects of Sulfur-Containing Compounds on Redox Status in Homocysteine-Treated Rats

Sobot¹,

Živković²,

Srejović³

et al. 2019

Acta Poloniae Pharmaceutica - Drug Research

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There is growing interest in the activity of sulfur-containing compounds on redox balance in physiological and pathological conditions, considering that some of these compounds have not only antioxidative but also pro-oxidative activities. The aim of this study was to assess possible differences in the effects of various sulfur-containing compounds on redox balance of cardiovascular system in its physiological state and in the early onset of hyperhomocysteinemia. This experimental study divided Wistar albino rats into two groups: saline-treated (control) and DL-homocysteine-treated (experimental group). Rats from experimental group were subjected to subchronic subcutaneous administration of DL-homocysteine at dose of 0.45 Ïmol/g body weight twice a day for 2 weeks. At the end of this period, rats were sacrificed, and blood samples were collected to be analyzed for homocysteine concentration and systemic oxidative stress. Isolated rat hearts were excised and attached to the Langendorff apparatus. To assess the effects of acute administration of L-methionine, L-cysteine, N-acetylcysteine, and sodium hydrogen sulfide, the hearts were perfused individually with each of the mentioned substances at the same single dose of 0.5 mmol/L for 5 min. In collected samples of coronary venous effluent oxidative stress biomarkers were determined using spectrophotometry. Total homocysteine level was significantly higher in the experimental group than in the control group, and the effects of applied sulfur-containing compounds were significantly different in experimental and control groups. DL-homocysteine induced considerable changes in the functioning of a cardiovascular system even before an increase in plasma homocysteine values, and action of sulfur-containing compounds varied depending on the presence of homocysteine.

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Section: Discussionsupporting

confidence: 91%

The Effects of Sulfur-Containing Compounds on Redox Status in Homocysteine-Treated Rats

Sobot¹,

Živković²,

Srejović³

et al. 2019

Acta Poloniae Pharmaceutica - Drug Research

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“…To assess whether compound 1 could induce any morphological changes, HepG2 cells were incubated with different concentrations of compound 1 . The cells were then observed and photographed using a fluorescence microscope after being stained with 3,3′-dioctadecyloxacarbocyanine perchlorate (Dio) and Hoechst 33342 [ 12 , 13 ]. As shown in Figure 4 A, compound 1 altered the cellular morphology considerably regardless of the dose.…”

Section: Resultsmentioning

confidence: 99%

Absolute Configuration and Biological Evaluation of Novel Triterpenes as Possible Anti-Inflammatory or Anti-Tumor Agents

Wei

Zhou

Xia³

et al. 2022

Molecules

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Two new compounds, ardisiapunine B (1) and ardisiapunine C (2), were isolated from Ardisia lindleyana D. Dietr. Their structures were examined using HR–ESI–MS, IR, (1D, 2D) NMR spectroscopic analyses, single–crystal X–ray diffraction, and ECD calculation. It was found that the two new compounds belong to unusual oleanane-type triterpenes, with compound 1 bearing an acetal unit and a C–13–C–18 double bond, and compound 2 bearing a C–28 aldehyde group and a C–18–C–19 double bond. The anti-inflammatory properties of compounds 1 and 2 were tested on NO production and cellular morphology using RAW264.7 cells, and their anti-tumor properties were tested on cytotoxic activities, cellular morphology, cell apoptosis, and cell cycle. The results showed that compound 1 exhibited a potent cytotoxicity against HepG2 cell lines with an IC50 of 12.40 μM. Furthermore, it is possible that compound 1 inhibits cell proliferation by blocking the cell G2/M phase and promoting cell apoptosis. Compound 2 exhibited a potential anti-inflammatory activity by decreasing the production of NO in LPS–stimulated RAW264.7 cells. Comparative analysis of the structures of compounds 1 and 2 revealed that the acetal structure and double bond positions were the main differences between them, and these are presumed to be the main reasons for the extreme differences in their cytotoxicity and anti-inflammatory activities. From these new findings, two promising lead compounds were identified for the future development of potential anti–inflammatory or anti–tumor agents.

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“…With the presence of U0126, an inhibitor of MEK1/2, the cultured neural progenitors were decreased after OGD compared with the one without U0126. In the research of Chang et al ( 2017 ), the neurite outgrowth was promoted by ARDD-activated ERK phosphorylation, which could be reversed by PD98059, an inhibitor of the ERK pathway. In Sun et al’s ( 2017 ) research, the neuronal apoptosis was significantly affected by NGF/HO-1-activated MEK/ERK pathway after the cultured primary cortical neurons were exposed to OGD.…”

Section: Discussionmentioning

confidence: 99%

Netrin-1 Prevents Rat Primary Cortical Neurons from Apoptosis via the DCC/ERK Pathway

Chen

Zhang

et al. 2017

Front. Cell. Neurosci.

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In the nervous system, Netrin-1 serves as a neural guide, mediating the neuronal development. However, it remains blurred whether Netrin-1 can protect neurons from apoptosis induced by cerebral stroke. In the current study, the cultured rat primary cortical neurons were transfected with Netrin-1-encoding lentivirus before the oxygen-glucose-deprivation (OGD) treatment. Cell death and apoptosis were evaluated by lactate dehydrogenase (LDH) release and flow cytometry. We found that Netrin-1 attenuated OGD-induced cell death and neuronal apoptosis at 24 h after OGD treatment, and that the overexpression of Netrin-1 activated the ERK signaling pathway. These effects were partly abolished by blocking its receptor deleted in colorectal cancer (DCC) or U0126, an inhibitor of the ERK signaling pathway. Netrin-1 overexpression in neurons elevated the expression of DCC, on mRNA level and protein level. Netrin-1 also reduced DNA damage. Taken together, our findings suggest that Netrin-1 attenuates cell death and neuronal apoptosis via the DCC/ERK signaling pathway in the cultured primary cortical neurons after OGD injury, which may involve the mediation of DNA damage in the neurons.

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The natural product 4,10-aromadendranediol induces neuritogenesis in neuronal cells in vitro through activation of the ERK pathway

Cited by 17 publications

References 33 publications

The Effects of Sulfur-Containing Compounds on Redox Status in Homocysteine-Treated Rats

The Effects of Sulfur-Containing Compounds on Redox Status in Homocysteine-Treated Rats

Absolute Configuration and Biological Evaluation of Novel Triterpenes as Possible Anti-Inflammatory or Anti-Tumor Agents

Netrin-1 Prevents Rat Primary Cortical Neurons from Apoptosis via the DCC/ERK Pathway

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