The nervous system component of the mesentery of the sea cucumber Holothuria glaberrima in normal and regenerating animals

Nieves-Ríos, Christian; Alvarez-Falcón, Samuel; Malavez, Sonya; Rodriguez-Otero, Jannette; García‐Arrarás, José E.

doi:10.1007/s00441-019-03142-3

Cited by 12 publications

(8 citation statements)

References 31 publications

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“…This activity could also be associated with downregulation of neurogenic associated transcripts observed when comparing day 3 versus 1 (see Supplementary Table S8 online). Previous studies from our group have shown that an important plexus made up of nerves and fibers is present in the intestinal mesentery 30 . Following evisceration, the plexus connection to the intestinal tissues is lost, since the latter have been eliminated.…”

Section: Discussionmentioning

confidence: 78%

“…Additional comparison between day 1 and 3 of regeneration using day 1 as the reference gave interesting results concerning downregulated nervous system genes (see Supplementary Table S8 online). Previous studies from our lab have shown that the nervous system within the mesentery is disrupted early in regeneration and slightly retracts or degenerates in the area adjacent to the evisceration rupture 30 . A decrease in the expression of nervous system-associated genes concurs with a negative effect on the mesentery nervous component.…”

Section: Differential Expression Analysismentioning

confidence: 88%

“…Thus, many of the axons connecting the mesentery to the intestine would have been severed. Morphological changes induced by the evisceration have been documented including the disorganization of the fiber tracts and their retraction at the regenerating end 30 . Therefore, it can be hypothesized that significant changes in nervous system gene expression are taking place during early regeneration stages.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Quispe-Parra

Medina-Feliciano

Cruz-González

et al. 2021

Sci Rep

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Echinoderms comprise a group of animals with impressive regenerative capabilities. They can replace complex internal organs following injury or autotomy. In holothurians or sea cucumbers, cellular processes of intestinal regeneration have been extensively studied. The molecular machinery behind this faculty, however, remains to be understood. Here we assembled and annotated a de novo transcriptome using RNA-seq data consisting of regenerating and non-regenerating intestinal tissues from the sea cucumber Holothuria glaberrima. Comparisons of differential expression were made using the mesentery as a reference against 24 h and 3 days regenerating intestine, revealing a large number of differentially expressed transcripts. Gene ontology and pathway enrichment analysis showed evidence of increasing transcriptional activity. Further analysis of transcripts associated with transcription factors revealed diverse expression patterns with mechanisms involving developmental and cancer-related activity that could be related to the regenerative process. Our study demonstrates the broad and diversified gene expression profile during the early stages of the process using the mesentery as the focal point of intestinal regeneration. It also establishes the genes that are the most important candidates in the cellular processes that underlie regenerative responses.

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Section: Discussionmentioning

confidence: 78%

Section: Differential Expression Analysismentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Quispe-Parra

Medina-Feliciano

Cruz-González

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…This activity could also be associated with downregulation of neurogenic associated transcripts observed when comparing day 3 vs 1 (see Supplementary Table S8 online). Previous studies from our group have shown that an important plexus made up of nerves and fibers is present in the intestinal mesentery 30 . Following evisceration, the plexus connection to the intestinal tissues is lost, since the latter have been eliminated.…”

Section: Discussionmentioning

confidence: 78%

Transcriptomic Analysis of Early Stages of Intestinal Regeneration inHolothuria glaberrima

Quispe-Parra¹,

Medina-Feliciano

Cruz-González

et al. 2020

Preprint

Self Cite

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Echinoderms comprise a group of animals with impressive regenerative capabilities. They can replace complex internal organs following injury or autotomy. In holothurians or sea cucumbers, cellular processes of intestinal regeneration have been extensively studied. The molecular machinery behind this faculty, however, remains to be understood. Here we assembled and annotated a de novo transcriptome using RNA-seq data consisting of regenerating and non-regenerating intestinal tissues from the sea cucumber Holothuria glaberrima. Comparisons of differential expression were made using the mesentery as a reference against 24 hour and 3 days regenerating intestine, revealing a large number of differentially expressed transcripts. Gene ontology and pathway enrichment analysis showed evidence of increasing transcriptional and translational activity. Further analysis of transcripts associated with transcription factors revealed diverse expression patterns with mechanisms involving developmental and cancer-related activity that could be related to the regenerative process. Our study demonstrates the broad and diversified gene expression profile during the early stages of the process using the mesentery as the focal point of intestinal regeneration. It also establishes the genes that are the most important candidates in the cellular processes that underlie regenerative responses.

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“…Coelomic epithelium is mainly composed of peritoneocytes and myoepithelial cells [ 51 , 54 ] with adjacent outer connective tissue. Some researchers refer to the coelomic epithelium, outer connective tissue, and muscle layer together as the mesothelium [ 55 ]. Recent studies suggest that there are three main tissue layers: the mesothelium, a connective tissue layer, and the luminal epithelium [ 23 ].…”

Section: Histology and Cytology Of Intestinal Regenerationmentioning

confidence: 99%

A Review of Histocytological Events and Molecular Mechanisms Involved in Intestine Regeneration in Holothurians

Yang

Sun

2022

Biology

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Most species of the class Holothuroidea are able to regenerate most of their internal organs following a typical evisceration process, which is a unique mechanism that allows sea cucumbers to survive in adverse environments. In this review, we compare autotomy among different type of sea cucumber and summarize the histocytological events that occur during the five stages of intestinal regeneration. Multiple cytological activities, such as apoptosis and dedifferentiation, take place during wound healing and anlage formation. Many studies have focused on the molecular regulation mechanisms that underlie regeneration, and herein we describe the techniques that have been used as well as the development-related signaling pathways and key genes that are significantly expressed during intestinal regeneration. Future analyses of the underlying mechanisms responsible for intestinal regeneration should include mapping at the single-cell level. Studies of visceral regeneration in echinoderms provide a unique perspective for understanding whole-body regeneration or appendage regeneration.

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The nervous system component of the mesentery of the sea cucumber Holothuria glaberrima in normal and regenerating animals

Cited by 12 publications

References 31 publications

Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Transcriptomic Analysis of Early Stages of Intestinal Regeneration inHolothuria glaberrima

A Review of Histocytological Events and Molecular Mechanisms Involved in Intestine Regeneration in Holothurians

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