The nia gene of Fusarium oxysporum: isolation, sequence and development of a homologous transformation system

Diolez, Annick; Langin, Thierry; Gerlinger, Catherine; Brygoo, Yves; Daboussi, Marie‐Josée

doi:10.1016/0378-1119(93)90669-t

Cited by 41 publications

(23 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Detection of transposable elements Flipper and Boty: The presence of the transposable elements Flipper, a 1872-bp class II element , and Boty, a 6-kb retrotransposon (Diolez et al, 1995), previously sporulation, M2 -aerial mycelium with sporulation, M3 -mycelial masses, and M4 -thick and woolly mycelium) and sclerotial (row below -left to right: S1-sclerotia at the edge of Petri dish, S2 -sclerotia large and arranged in a circle, S3 -sclerotia large, placed irregularly, and S4 -sclerotia small and scattered) identified in the B. cinerea genome, was tested in all obtained isolates using PCR methods described by Levis et al (1997) and Ma & Michailides (2005). The primers used for detection of the Flipper element (F300: 5'-GCA CAA AAC CTA CAG AAG A-3' and F1550: 5'-ATT CGT TTC TTG GAC TGT A-3') amplify a 1250-bp product, corresponding to a major part of the Flipper element.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Botrytis cinerea in raspberry in Serbia I: Morphological and molecular characterization

Tanović

Hrustić

Mihajlović

et al. 2014

Pesticidi i fitomedicina

View full text Add to dashboard Cite

SuMMaryMorphological and molecular characterisation of 130 isolates of Botrytis cinerea, derived from raspberry fruit originating from six commercial fields in a raspberry growing region of Serbia (locations: Požega, Prilike, Arilje, Ivanjica, Šabac and Valjevo) was performed. The results showed that all isolates formed white, uniform, aerial mycelia with entire margin on PDA medium. First morphological differences among the isolates appeared after six days of incubation. Three-week old isolates were grouped into eight distinct morphological types -four mycelial and four sclerotial. Mostly, they were of sclerotial type (81.5%) and the most frequently found was an S3 type, which formed large irregularly placed sclerotia. This type was dominant in five of six investigated locations and represented 45-65% of the isolates. The least frequent was the mycelial type M3 (0.7% of the isolates) characterized by mycelial masses.The presence of Boty and/or Flipper transposons was detected in isolates originating from all investigated locations. It was discovered that the B. cinerea population in raspberry in Serbia, besides the well-described genetically isolated sympatric species transposa (43.1%) and vacuma (10.8%), contains also another two, boty (44.6%) and flipper (1.5%) species with only one transposon (either Boty or Flipper) in the genome. In addition, it was revealed that all isolates from raspberry collected in Serbia, transposa, vacuma, boty or flipper, are sensitive or weakly resistant to fenhexamid and therefore belong to the B. cinerea genetical Group II.

show abstract

Section: Molecular Characterizationmentioning

confidence: 99%

Botrytis cinerea in raspberry in Serbia I: Morphological and molecular characterization

Tanović

Hrustić

Mihajlović

et al. 2014

Pesticidi i fitomedicina

View full text Add to dashboard Cite

show abstract

“…Plasmid pNH24 was described by Diolez et al (5) and has an insert of about 4.0 kb containing the Fusarium oxysporum nitrate reductase gene and unique sites for the enzymes Xba I and Eco RI.…”

Section: Transformation Vectormentioning

confidence: 99%

“…: (+5531) 3899-2553, Fax: (+5531) 3899-2573. E-mail: mvqueiro@mail.ufv.br oryzae (15,28), Fusarium oxysporum (5,13), Gibberella fujikuroi (23,27), Penicillium chrysogenum (7,35), and P. griseoroseum (16,19), among others. In the heterologous transformation systems, the transformation frequency was lesser than 20 transformants/µg DNA.…”

Section: Introductionmentioning

confidence: 99%

Development of a transformation system for Penicillium brevicompactum based on the Fusarium oxysporum nitrate reductase gene

Varavallo¹,

Queiroz²,

Pereira³

et al. 2005

Braz. J. Microbiol.

View full text Add to dashboard Cite

Penicillium brevicompactum is a filamentous fungus that presents a potential for industrial use due its efficient pectinase production. A heterologous transformation system was developed for P. brevicompactum based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. One mutant named 4457-18X was chosen for the transformation experiments with the pNH24 vector containing de Fusarium oxysporum nitrate reductase gene. A frequency of approximately 3 transformants/µg DNA was obtained using the circular vector pNH24. This frequency was multiplied about 10 fold using the linearized vector with the Xba I restriction enzyme. Southern analysis of the transformants showed a tendency of the linearized vector to diminish the number of integrations compared to the use of the circular vector. The integration was random and stable in the analyzed transformants. The establishment of a transformation system for P. brevicompactum is fundamental for genetic manipulation of this microorganism.

show abstract

“…The vectors used were plasmid pNOM 102 (Roberts et al, 1989) containing the E. coli β-glucuronidase gene (gusA) flanked by a glyceraldehyde 3-phosphate promoter (gpd) from Aspergillus nidulans upstream and the A. nidulans trpC transcription termination signal downstream and plasmid pNH24 (Diolez et al, 1993) carrying the F. oxysporum nitrate reductase gene (nia) subcloned into plasmid pUC19. The pUC plasmid (without the F. oxysporum nia gene) was used as a negative control to test for strain stability.…”

Section: Vectorsmentioning

confidence: 99%

Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes

2004

View full text Add to dashboard Cite

A tropical endophytic isolate of the fungus Fusarium verticillioides (synonym Fusarium moniliforme) obtained from Zea mays was co-transformed with plasmid pNH24 containing the Fusarium oxysporum nitrate reductase nia gene and plasmid pNOM 102 carrying the Escherichia coli β-glucuronidase gusA gene. Transformation frequency for the nia marker was 75 transformants µg -1 vector DNA and introduction of the gusA gene by co-transformation was 57.2% as indicated by the presence of the GUS + phenotype on plates. Southern analyses confirmed the integration of both plasmids into the genome of ten GUS + transformants. All co-transformants showed mitotic stability in respect of the GUS + phenotype. To assess the potential of transformed endophytic fungi as vectors for introducing desirable characteristics into host tropical plants of biotechnological and agricultural importance we successfully infected maize roots and detected GUS + phenotype".

show abstract

The nia gene of Fusarium oxysporum: isolation, sequence and development of a homologous transformation system

Cited by 41 publications

References 27 publications

Botrytis cinerea in raspberry in Serbia I: Morphological and molecular characterization

Botrytis cinerea in raspberry in Serbia I: Morphological and molecular characterization

Development of a transformation system for Penicillium brevicompactum based on the Fusarium oxysporum nitrate reductase gene

Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes

Contact Info

Product

Resources

About