The nucleoporin NUP160 and NUP96 regulate nucleocytoplasmic export of mRNAs and participate in ethylene signaling and response in Arabidopsis

Abstract

The two-way transport of mRNAs between the nucleus and cytoplasm are controlled by the nuclear pore complex (NPC). In higher plants, the NPC contains at least 30 nucleoporins. The Arabidopsis nucleoporins are involved in various biological processes such as pathogen interaction, nodulation, cold response, owering, and hormone signaling. However, little is known about the regulatory functions of the nucleoporin NUP160 and NUP96 in ethylene signaling pathway. In the present study, we provided data showing that the Arabidopsis nucleoporin NUP160 and NUP96 participate in ethylene signaling-related mRNAs nucleocytoplasmic transport. The Arabidopsis nucleoporin mutants (nup160, nup96-1, nup96-2) exhibited enhanced ethylene sensitivity. Nuclear qRT-PCR analysis and poly(A)-mRNA in situ hybridization showed thatthe nucleoporin mutants affected the nucleocytoplasmic transport of all the examined mRNAs, including the ethylene signaling-related mRNAs such as ETR2, ERS1, ERS2, EIN4, CTR1, EIN2, and EIN3. Transcriptome analysis of the nucleoporin mutants provided clues suggesting that the nucleoporin NUP160 and NUP96 may participate ethylene signaling via various molecular mechanisms. These observations signi cantly advance our understanding of the regulatory mechanisms of nucleoporin proteins in ethylene signaling and ethylene response.

The Time-Resolved Salt Stress Response of Dunaliella tertiolecta—A Comprehensive System Biology Perspective

“…HAT-repeatcontaining proteins are required for RNA processing, as this motif is found in proteins related to RNA metabolism, such as RNA splicing, rRNA biogenesis, and polyadenylation [57,58]. Another protein related to RNA biogenesis is the nuclear pore protein 96, which is involved in mRNA export [59]. The increased amount of these two proteins might support the RNA processing to synthesize new proteins, necessary for adapting to high salt concentrations.…”

The scaffold nucleoporins SAR1 and SAR3 are essential for proper meiotic progression in Arabidopsis thaliana

“…The outer ring complex plays essential roles in NPC assembly, microtubule polymerization at kinetochores, and DNA repair ( Walther et al, 2003 ; Nagai et al, 2008 ; Mishra et al, 2010 ). In Arabidopsis thaliana , members of this complex are involved in flowering time regulation, abiotic stress and immune responses, as well as in hormone signaling ( Gu, 2018 ; Zhang A. et al, 2020 ; Cheng et al, 2020 ; Nie et al, 2023 ). In this species, defective mutants for members of this complex, such as nup160 and nup96 , were identified in a screening for suppression of auxin resistance phenotypes.…”