The nucleotide sequence of the ubiquitous repetitive DNA sequence B1 complementary to the most abundant class of mouse fold-back RNA

Abstract

Three copies of a highly repetitive DNA sequence B1 which is complementary to the most abundant class of mouse fold-back RNA have been cloned in pBR322 plasmid and sequenced by the method of Maxam and Gilbert. All the three have a length of about 130 base pairs and are very similar in their base sequence. The deviation from the average sequence is equal to 4% and the overall mismatch between each two is not higher than 8%. One of the recombinant clones used contained two copies of B1 oriented in the same direction. All of the B1 copies are flanked with sequences which possess nonidentical but very similar structure. They consist of a number of AmCn blocks (where m varies from 2 to 8 and n equals 1-2). These peculiar sequences in all cases are separated from B1 by non-homologous DNA stretches of 2-8 residues. In one case, a long polypurine stretch is located next to such a block. It consists of 74 residues most of which represent a reiteration of the basic sequence AAAAG. We have found two regions within the B1 sequence which are homologous to the intron-exon junctions, especially to those present in the large intron of the mouse beta-globin gene. It may indicate the involvement of the B1 sequence in pre-mRNA splicing.

Origin and evolution of SINEs in eukaryotic genomes

“…identified only in rodents (Krayev et al, 1980;Veniaminova et al, 2007), primates (Deininger et al, 1981;Zietkiewicz et al, 1998) and tree shrews (Nishihara et al, 2002;Vassetzky et al, 2003). The 7SL RNA (B300 nt) is found in all eukaryotes as the RNA component of the signal recognition particle (SRP), the ribonucleoprotein that targets secreted proteins to the endoplasmic reticulum.…”

Characterization of Two Age-induced Intracisternal A-particle-related Transcripts in the Mouse Liver

“…These are highly reiterated mobile elements of the mouse genome classically found in intronic domains, and which are closely related to the human Alu sequences (21,22). All repeats except one are in an opposite transcriptional orientation as compared to IAP-AR1.…”

Repetitive sequences in the rat genome that have cleavage sites for the restriction endonuclease Taq I.

“…Therefore, the Alu type repeated DNA of humans ), mouse B1 (Krayev et al 1980), chinese hamster (Haynes and Jelinek 1981) and non-Alu family repeats, Kpn I family (Digiovanni et al 1983) and Hinf I family (Shimizu et al 1983) in humans, were compared to the consensus sequence of four clones (Kikuchi et al 1987). However, the 0.29 kb Taq I repeat sequence of the rat was not the member of Alu family repeat, and did not show any homology to other repetitive families (data not shown).…”