The nutrients in Moringa oleifera leaf contribute to the improvement of stylo and alfalfa silage: Fermentation, nutrition and bacterial community

He, Liwen; Lv, Hongjian; Xing, Yaqi; Cheng, Wang; You, Xiao‐Zeng; Chen, Xiaoyang; Zhang, Qing

doi:10.1016/j.biortech.2020.122733

Cited by 30 publications

(27 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The specific changes were reflected by the differences in bacterial composition at the genus level among different treatments stylo silage. Some previous reports consistent with this study, additives such as Moringa oleifera powder and gallic acid shaped the bacterial community structure ( He et al, 2020a , c ). In particular, the structure of the bacterial communities greatly at the genus level.…”

Section: Discussionsupporting

confidence: 91%

“…The pH value of additive-treated silages was close to 4.2, ensuring the well preservation of stylo silage ( Edwards and McDonald, 1978 ). A previous study has shown higher pH values at 5.0 or above in stylo silage, which is maybe caused melatonin treatment more efficient than Moringa oleifera leaves or gallic acid addition ( He et al, 2020a , c ). Lactic acid in silage is the dominant product from fermentation, which is an important index to evaluate silage quality.…”

Section: Discussionmentioning

confidence: 98%

“…However, the NH 3 -N contents of stylo silage were various. One study reported the NH 3 -N in stylo and Moringa oleifera leaves mixed silage were range from 5.07 to 11.1%( He et al, 2020c ). In contrary, gallic acid reduced NH 3 -N from 1.46 to 0.29% ( He et al, 2020a ).…”

Section: Discussionmentioning

confidence: 99%

“…Specimens were heated at 65°C for 72 h, and dried materials were ground for chemical analysis. The contents of dry matter (DM), crude protein (CP), water-soluble carbohydrates (WSC), neutral detergent fiber (NDF) and acid detergent fiber (ADF) were examined using previously established methods ( He et al, 2020c ). The contents of DM, CP, WSC, NDF and ADF in stylo before ensiled were 30.10, 10.49, 1.23, 51.04, and 40.76%, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Due to the influence of climate and environmental factors, stylo production is highly seasonal, which limits its popularization and utilization and ensiling is really essential to ensure the balanced annual supply of livestock feed. Conventional additives have been reported in stylo silage, which can improve the fermentation quality and affect the microbial community of silage ( Li et al, 2017 ; He et al, 2020c ). In view of the characteristics of melatonin and its application in fruit and food preservation, we hypothesized that melatonin could regulate the fermentation quality of stylo silage by altering the microorganisms and metabolites.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Melatonin Is a Promising Silage Additive: Evidence From Microbiota and Metabolites

Zhang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

The safe and effective storage of forage are very important. As an important storage method, ensiling can keep fresh forage for a long time with less nutritional loss. Melatonin has antioxidant and bacteriostasis, usually used as a natural preservative. The influence of melatonin on silage microbial or fermentation quality has not been clarified. In the present study, we aimed to clarify whether melatonin affected stylo (Stylosanthes guianensis) silage quality via microbiota and metabolites. Melatonin addition significantly improved the silage fermentation quality, including the increased contents of lactic acid and total acid (244.18–255.81% and 63.95–78.97%, respectively), as well as the decreased in pH and butyric acid content compare with control group. Moreover, 16S rRNA sequencing indicated that melatonin addition enhanced the silage microbial diversity indices (such as increase in Shannon indices but decrease in Simpson indices), and significantly shaped the composition of silage microbiota (such as increased abundances of Pantoea, Stenotrophomonas, Sphingobacterium, and Pseudomonas, and decreased abundance of Weissella). Melatonin addition also dramatically affected the metabolites of sylo silage, such as raised malonic acid and some amino acid metabolism(glycine, threonine, methionine and ornithine), while reduced nucleic acid metabolism(2-deoxyuridine and thymine) and carbon metabolism(allose and 2-deoxy-D-glucose). Collectively, our results confirmed that the lowest melatonin addition (5 mg/kg) could improve the fermentation quality, and the potential mechanisms might be associated with the microbiota and metabolites in stylo.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Melatonin Is a Promising Silage Additive: Evidence From Microbiota and Metabolites

Zhang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

Additives improve the fermentation quality, anthocyanin content, and biological activity of purple Napier grass silage

Yin,

Wu,

Shen

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDPurple Napier grass (PNG), a widely used grass rich in anthocyanin, is commonly employed in the production of silage. However, there is currently limited research on the retention of anthocyanin with or without additives during ensiling. Therefore, this study aimed to investigate the effect of different additives (Lactiplantibacillus plantarum CCZZ1 (LP), glucose, acetic acid, and dried soybean curd residue) on fermentation quality, anthocyanin content, and microbial community structure of PNG silage.RESULTSEnsiling PNG without additives led to poor fermentation quality and rapid degradation of anthocyanin, resulting in a decline in antioxidant activity and the persistence of harmful microorganisms with high relative abundance. The use of additives, especially LP, effectively increased the relative abundance of L. plantarum, enhancing fermentation quality, the retention of anthocyanin (up to 166% increase rate) and antioxidant activity, while reducing the relative abundance of harmful microorganisms during ensiling for 30 days. Additionally, prolonged ensiling negatively affected the preservation of anthocyanin. Based on both fermentation quality and bioactivity, PNG should be ensiled for 30 days with LP inoculation.CONCLUSIONThe employment of additives, especially LP, improved the fermentation quality, anthocyanin retention, and microbial community structure in PNG silage. To optimize both fermentation quality and bioactivity, it is recommended that PNG be ensiled for 30 days with LP inoculation. © 2024 Society of Chemical Industry.

show abstract

Effects of polyphenol oxidases on proteolysis and lipolysis during ensiling of Moringa oleifera leaves with or without pyrocatechol

Wang

Pian

Chen

et al. 2021

Animal Feed Science and Technology

View full text Add to dashboard Cite

The nutrients in Moringa oleifera leaf contribute to the improvement of stylo and alfalfa silage: Fermentation, nutrition and bacterial community

Cited by 30 publications

References 29 publications

Melatonin Is a Promising Silage Additive: Evidence From Microbiota and Metabolites

Melatonin Is a Promising Silage Additive: Evidence From Microbiota and Metabolites

Additives improve the fermentation quality, anthocyanin content, and biological activity of purple Napier grass silage

Effects of polyphenol oxidases on proteolysis and lipolysis during ensiling of Moringa oleifera leaves with or without pyrocatechol

Contact Info

Product

Resources

About