The nutrition of the larva of Aëdes aegypti Linnaeus. 3. Lipid requirements

Expression of genes encoding proteins involved in ecdysteroidogenesis in the female mosquito, Aedes aegypti

“…Human plasma lipoproteins contain both cholesterol esters and free cholesterol (Gibbons et al, 1982;Oh, 1982), both of which have been shown to be metabolically usable by A. aegypti larvae (Golberg and De Meillon, 1948). Ovaries in female A. aegypti could acquire cholesterol needed for ecdysteroid biosynthesis either by a lipophorin receptormediated process or through ''aqueous diffusion'' Capurro Mde et al, 1994;Ford and Van Heusden, 1994;Van Heusden et al, 1997;van Heusden et al, 1998;Sun et al, 2000;Cheon et al, 2001;Jouni et al, 2003).…”

An Inexpensive and Effective Larval Diet forAnopheles arabiensis(Diptera: Culicidae): Eat Like a Horse, a Bird, or a Fish?

“…The presence of these components is essential to all artiÞcial diets. At least 14 amino acids are known to be essential for mosquito larval development and survival (Goldberg and De Meillon 1948, Singh and Brown 1957, Dadd 1985, Vrzal et al 2010. Because of their inability to elongate the 18C PUFAs, mosquito diets must include C18, C20, and C22 PUFAs, described as essential for larval development (Moribayashi et al 2004) and for mosquito ßight and adult survival (Dadd and Kleinjan 1979).…”

Studies on the Nutritional Relationship of Larval Aedes aegypti (Diptera: Culicidae) with Smittium culisetae (Trichomycetes)

“…Golberg and De Meillon (1948) similarly found yeast lipids to be toxic when in excess, as did Akov (1962) with cholesterol. Larvae which otherwise would never have developed beyond the fourth instar without sterols pupated with the addition of either the lipid extract or dead whole mycelium of S. culisetae to the sterol-free semidefined medium.…”

“…Larvae were capable of pupation with desmosterol as the sole sterol in the semidefined medium but mortality was considerably greater than with cholesterol Golberg and De Meillon (1948). examined, with ergosterol and cholesterol only occasionally being detected.…”