The occurrence of fungi in bovine tissues in relation to portals of entry and environmental factors

Jensen, H. E.; Aalbæk, Bent; Basse, Astrid L.; Schønheyder, Henrik Carl

doi:10.1016/0021-9975(92)90030-x

Cited by 17 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, some fungal species present in silage have been linked to mycotic infection in cattle, in particular Aspergillus fumigatus (Jensen et al . ; Mansfield and Kuldau ), which is an opportunistic human pathogen, and an agent of aspergillosis in immune‐compromised individuals (Dagenais and Keller ).…”

Section: Introductionmentioning

confidence: 99%

“…The growth of filamentous fungi in silages is often associated with the production of mycotoxins (Auerbach et al 1998;Storm et al 2008), harmful to cattle, farm workers and dairy product consumers (Fink-Gremmels 2008). Furthermore, some fungal species present in silage have been linked to mycotic infection in cattle, in particular Aspergillus fumigatus (Jensen et al 1992;Mansfield and Kuldau 2007), which is an opportunistic human pathogen, and an agent of aspergillosis in immune-compromised individuals (Dagenais and Keller 2009). Knowledge about microbial successions during and after the fermentation process of silage has been restricted by the lack of suitable methods that enable differentiation among individual microbial species (Stevenson et al 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evolution of fungal populations in corn silage conserved under polyethylene or biodegradable films

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evolution of fungal populations in corn silage conserved under polyethylene or biodegradable films

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In most of these techniques, polyclonal antibodies have been applied as the primary reagents, 12,13,15,19,21,22,24,26,32,33 whereas monoclonal antibodies (MAbs) have only been utilized for a limited number of fungal species and a few studies. 6,7,16,20,23,34,40 The use of polyclonal antibodies rather than MAbs in immunodiagnostic techniques has some disadvantages, e.g., the time-consuming and labor-intensive heterologous absorption of polyclonal antibodies, which is necessary to achieve sufficient specificity.…”

mentioning

confidence: 99%

Development of Murine Monoclonal Antibodies for the Immunohistochemical Diagnosis of Systemic Bovine Aspergillosis

Jensen¹,

Aalbæk

Lind³

et al. 1996

J VET Diagn Invest

View full text Add to dashboard Cite

Abstract. Murine monoclonal antibodies (MAbs) against water-soluble somatic antigens (WSSA) and the wall fraction (WF) from Aspergillus fumigatus were produced by fusion of splenocytes from immunized BALB/c mice with mouse myeloma X63-Ag 8.653 cells. The supernatants of in vitro cultured hybridomas were initially screened for reactivity with the WSSA and the WF from A. fumigatus and WSSA of other fungi in an enzymelinked immunosorbent assay (ELISA). Supernatants reacting only with A. fumigatus antigens were subsequently screened for homologous and heterologous reactivity with immunohistochemical techniques using formalinfixed, paraffin-embedded tissues from experimentally infected mice. Because of a high immunohistochemical reactivity with homologous fungi, 4 MAbs raised against A. fumigatus WSSA and WF were selected for a further evaluation of cross-reactivity (diagnostic specificity) in immunohistochemical and immunoblotting assays. In immunohistochemical assays, all MAbs raised against WSSA cross-reacted heavily with a number of other fungal species. All 4 MAbs (MAb-WF-AF-1-4) raised against the WF reacted strongly with hyphae of Aspergillus spp.; hyphae of Scedosporium apiospermum were also strongly labeled by MAb-WF-AF-3 and-4. The 2 specifically reacting MAbs (MAb-WF-AF-1 and-2) were of the IgM biotype and were precipitating, and in immunoblotting experiments both bound to a 106-kD antigen of the WF, whereas they did not bind to WSSA of A. fumigatus. One of the 2 aspergillosis-specific MAbs (MAb-WF-AF-1) was used to screen 145 mycotic lesions of cattle. The diagnoses on bovine lesions obtained by MAb-WF-AF-1 were compared with results based on reactivity with heterologously absorbed polyclonal antibodies and, for some lesions, to culture results. In the vast majority of lesions (n = 133), the MAb-WF-AF-1 and the polyclonal anti-Aspergillus antibodies reacted in a similar pattern, i.e., positively in 41 aspergillosis lesions and negatively in 92 zygomycotic lesions. Hyphae in 3 of 12 lesions that were not stained by the polyclonal antibodies reacted with the specific MAb-WF-AF-1; i.e., aspergillosis was diagnosed. The characteristics of the 2 MAbs (MAb-WF-AF-1 and-2) raised against the WF of A. fumigatus in ELISA and immunoblotting and immunohistochemical assays justify their application for the in situ diagnosis of systemic aspergillosis of cattle.Aspergillus fuimigatus is the main cause of systemic bovine aspergillosis, a disease occuring worldwide and of great importance in cattle production. [2][3][4]14,[18][19][20][21]27,31,37 Because systemic mycoses including aspergillosis are difficult to diagnose clinically, most cases are not diagnosed until histopathologic evaluation is completed as part of a postmortem examination. 2-4,14,18-2l,27,3l,37 Attempts to culture the etiologic agents of systemic mycoses including Aspergillus spp. may give false results because of several difficulties, e.g., growth failure, overgrowth by contaminants, recovery of a fungus different from that suggested by the morpholo...

show abstract

“…However, isolation of fungi from animal tissue may be difficult because of post-mortem changes (Dion et aL, 1987). Moreover, judging whether an isolate is also the pathogen may be difficult owing to the risk of contamination and growth of dormant fungal spores (Cordes and Shortridge, 1968;Dion et al, 1987;Jensen et al, 1992;Lehman and White, 1975). Therefore, histopathological examination is essential to confirm infection (Jensen et aL, 1990a(Jensen et aL, ,1992.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, judging whether an isolate is also the pathogen may be difficult owing to the risk of contamination and growth of dormant fungal spores (Cordes and Shortridge, 1968;Dion et al, 1987;Jensen et al, 1992;Lehman and White, 1975). Therefore, histopathological examination is essential to confirm infection (Jensen et aL, 1990a(Jensen et aL, ,1992. A tentative aetiological diagnosis may be obtained from the morphological details of fungi within tissue sections but, as the appearance of hyphae in sections is altered by a number of factors, this is considered to be difficult (Cordes and Shortridge, 1968;Jensen and Schcnheyder, 1989;Jensen et aL, 1990aJensen et aL, ,c,1993Ossent, 1987).…”

Section: Introductionmentioning

confidence: 99%

Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle

Jensen

1993

Vet Res Commun

View full text Add to dashboard Cite

A novel method for diagnosing bovine aspergillosis and zygomycosis is described. Rabbit hyperimmune antisera raised against somatic antigens of Aspergillus fumigatus and Absidia corymbifera were used in crossed immunoelectrophoresis with supernatants from disintegrated tissues from acute necrohaemorrhagic mycotic lesions from cattle. The method specifically identified 4 of 5 lesions with aspergillosis and 2 of 5 lesions with zygomycosis. One lesion dually infected with aspergillosis and zygomycosis was negative. The method worked with unabsorbed sera, was specific, and required only standard electrophoretic equipment. It can therefore supplement chemical detection of fungi in tissues in the diagnosis of bovine aspergillosis and zygomycosis.

show abstract

The occurrence of fungi in bovine tissues in relation to portals of entry and environmental factors

Cited by 17 publications

References 18 publications

Evolution of fungal populations in corn silage conserved under polyethylene or biodegradable films

Evolution of fungal populations in corn silage conserved under polyethylene or biodegradable films

Development of Murine Monoclonal Antibodies for the Immunohistochemical Diagnosis of Systemic Bovine Aspergillosis

Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle

Contact Info

Product

Resources

About