The optimal concentration of topical hydroxycamptothecin in preventing intraarticular scar adhesion

Abstract

10-Hydroxycamptothecin could reduce intraarticular adhesion by inhibiting fibroblasts proliferation after knee surgery. However, the ideal concentration of hydroxycamptothecin have not been defined. This study was tried to verify the optimal concentration of 10-hydroxycamptothecin in preventing knee intraarticular adhesion. Sixty rabbits were randomly divided into five groups. Approximately 10 mm × 10 mm of the cortical bone was removed from both sides of the femoral condyle and the underneath cancellous bone was exposed. Various concentrations of hydroxycamptothecin (0.1 mg/ml, 0.5 mg/ml, 1.0 mg/ml, 2.0 mg/ml) or saline were applied to the decorticated areas for 10 minutes. After four weeks, the degree of inraarticular adhesion was assessed by macroscopic evaluation, biochemical analysis of hydroxyproline content and histological evaluation. The results demonstrated that the extent of knee inraarticular adhesion in 1.0 mg/ml group and 2.0 mg/ml hydroxycamptothecin group were significantly lower than those of 0.5 mg/ml group, 0.1 mg/ml hydroxycamptothecin group and control group. Moreover, there was no significant difference between 1.0 mg/ml group and 2.0 mg/ml hydroxycamptothecin group. In conclusion, topical application of 1.0 mg/ml hydroxycamptothecin may be the optimal concentration in reducing intraarticular adhesion after knee surgery in rabbits.

Prospective application of stem cells to prevent post‐operative skeletal fibrosis

“…Owing to the anti‐fibrotic effect of anti‐tumor drugs, positive results have been achieved in reducing post‐operative skeletal fibrosis. Previous studies have shown that topical application of anti‐tumor drugs, including mitomycin C (MMC), 10‐hydroxycamptothecine (HCPT), colchicine and 5‐fluorouracil (5‐Fu), could prevent post‐operative skeletal fibrosis in animal models by inhibiting the inflammatory response, suppressing fibroblast proliferation and reducing collagen deposition. However, more research is needed to determine the exact effect of 5‐Fu .…”

Intra-Articular Injection of Mitomycin C Prevents Progression of Immobilization-Induced Arthrogenic Contracture in the Remobilized Rat Knee

“…Extracellular matrix proteins, such as collagen, are produced by fibroblasts. In joint injury-induced contracture, inhibiting fibroblast proliferation by anti-proliferative agents MMC or hydroxycamptothecin can attenuate fibrosis in intraarticular adhesion sites (Li et al 2013b, Liang et al 2014. Therefore, it seems likely that fibroblast proliferation plays an important role in generating joint fibrosis.…”

Intra-Articular Adhesion Reduction after Knee Surgery in Rabbits by Calcium Channel Blockers

“…The rabbit knee intra-articular adhesion model was performed according to the previous protocol [ 3 ]. Sterile conditions were prepared beforehand.…”

“…Selecting 3 rabbits randomly per group, macroscopic evaluation was performed 4 weeks post-surgery. The surgical knee was reopened with re-anesthesia, and the presence and severity of intra-articular adhesions was evaluated, with the results based on the visual scoring system ( Table 1 ) [ 1 – 3 ].…”

“…No effective treatments are widely accepted [ 3 ]. A variety of methods have been developed to prevent intra-articular adhesions in animal models and humans, including arthroscopic lysis of adhesions, intra-articular and/or mitomycin C application, continuous corticosteroid administration, and hyaluronan derivative gel [ 4 – 8 ].…”